Reduced inattention and hyperactivity and improved cognition after marine oil extract (PCSO-524®) supplementation in children and adolescents with clinical and subclinical symptoms of attention-deficit hyperactivity disorder (ADHD): a randomised, double-blind, placebo-controlled trial

This study investigated the effects of a marine oil extract (PCSO-524®) on inattention, hyperactivity, mood and cognition in children and adolescents. PCSO-524® is a standardised lipid extract of the New Zealand green-lipped mussel and is an inflammatory modulator that inhibits the 5′-lipoxygenase and cyclooxygenase pathways and decreases concentrations of the pro-inflammatory arachidonic acid (AA). PCSO-524® or a matched placebo was administered for 14 weeks to 144 participants (123 males/21 females; mean age 8.7 years) with high hyperactivity and inattention in a randomised, double-blind, placebo-controlled study. The primary outcome was the Conners Parent Rating Scale assessing parental reports of behavioural problems. Secondary outcomes assessed changes in cognition and mood. The results of the present study did not support the hypothesis that PCSO-524® improves parental reports of hyperactivity, inattention and impulsivity in children ages 6 to 14 years over placebo. Repeated measures ANOVA on post hoc subsample analysis indicated significant improvements in hyperactivity (p = 0.04), attention (p = 0.02), learning (p = 0.05) and probability of ADHD (p = 0.04) with a medium to large average effect size (d = 0.65) in those children who did not meet criteria for combined hyperactivity and inattention. Furthermore, significant improvements in the PCSO-524® group were indicated in a whole sample repeated measures ANCOVA on recognition memory between baseline and week 8 over placebo (p = 0.02, d = 0.56); this difference was not sustained at week 14. The results presented indicate that PCSO-524® may be beneficial in reducing levels of hyperactivity and inattention in a population of children with clinical and subclinical symptoms of ADHD.


Introduction
Developmental disorders have detrimental effects on a child's social, emotional and academic future (Wehmeier et al. 2010). Attention-deficit/hyperactivity disorder (ADHD) is the most prevalent developmental disorder in school-aged children, with ADHD's prevalence being estimated within Western cultures to be between 5 and 12% (Biederman 2005;Wolraich et al. 2005). While ADHD is diagnosed by the use of criteria that establish clinical levels of hyperactivity, impulsivity and cognitive dysfunction related to inattention and impulsivity (American Psychiatric Association 2000), subclinical levels of these symptoms may still be sufficient to cause significant personal and social distress (Gadit 2003). In fact, subclinical ADHD has demonstrated to be a significant risk factor for alcohol and substance use disorders in later years (Shankman et al. 2009;Malmberg et al. 2011).
Children who present with ADHD symptoms at the subclinical level highlight an area of child mental health that is widespread and largely overlooked. Recent research has indicated a prevalence range for this cohort anywhere from 0.8 to 23% of the population (Balázs and Keresztény 2014). Identifying children with subclinical levels of ADHD may also aid in the broader understanding of gender differences in terms of symptom expression and severity (Rielly et al. 2006) as well as aid in the approach to treatment for those children who are at risk of developing the disorder (Kobor et al. 2012). As such, there is difficulty for parents to have a clear understanding of how to approach their child's behavioural issues and subsequently find appropriate interventions.
Current pharmacological treatments for ADHD can involve the administration of amphetamine-type and methylphenidate substances, which although efficacious are for many parents an undesirable option. Furthermore, for children who display milder or subclinical levels of inattention or hyperactivity, alternative to amphetamine-type stimulant treatment is arguably preferable. As such, a growing literature concerning alternative treatments proposed for children and adolescents with similar behavioural issues to those with ADHD is developing (Sarris et al. 2011). Although many of these alternative treatments have not been subjected to rigorous scientific clinical trials, there is growing evidence for marine-based intervention efficacy in treating behavioural issues associated with ADHD (Sarris et al. 2011).
One increasingly popular treatment for symptoms of ADHD is supplementation with long-chain polyunsaturated fatty acid (LC PUFAs). Some researchers have argued that these LC PUFAs are a viable alternative to methylphenidate (MPH, e.g. Ritalin®) and other stimulant and non-stimulant pharmaceutical treatments (Richardson 2006;Sinn and Bryan 2007). Longitudinal research into prenatal development has also shown that children who have low levels of docosahexaenoic acid (DHA) in cord blood have increased levels of inattention and hyperactivity at 10 years of age (Kohlboeck et al. 2011). LC PUFAs modify membrane fluidity, neurotransmitter release, cortical connectivity and organisation, as well as decrease levels of inflammatory mediators (Hariri et al. 2012). The involvement of LC PUFAs in processes related to neuronal maturation (Grayson et al. 2014) further suggests that LC PUFA consumption can positively influence these molecular processes and that these changes are also accompanied by behavioural improvements.
A recent clinical trial involving 40 boys with ADHD and 39 healthy controls compared the effect of 16-week administration of combined eicosapentaenoic acid (EPA)/DHA against placebo on behaviour and cognition (Bos et al. 2015). Significant improvements due to the treatment were observed for both the ADHD and control groups due to the EPA/DHA treatment on parent-rated inattention scores but not on the more objectively assessed cognitive processes (Bos et al. 2015). Research findings in this area have been somewhat mixed with several positive findings for LC PUFAs on behavioural outcomes in children and adolescents, as well as several failures to replicate (Sarris et al. 2011). Nevertheless, it should be noted that two recent meta-analyses have suggested positive but small effect sizes for LC PUFAs and behavioural symptoms with children and adolescents with ADHD (Sonuga- Barke et al. 2013;Bloch and Qawasmi 2011). One particular problem in understanding whether omega-3 supplements are efficacious in children with high hyperactivity, inattention and impulsivity is the lack of clarity with regards to the mechanism of action. One promising marine-based preparation that may benefit children and adolescents with behavioural problems and which is rich in LC PUFAs is PCSO-524®, which contains a standardised lipid extract of the New Zealand green-lipped mussel (Perna canaliculus). The extract contains a unique combination of free fatty acids, sterol esters, polar lipids and carotenoids that provide a highly condensed form of marine lipids (Kalafatis 1996). Experimental studies demonstrate that the PCSO-524® extract is effective at modulating 5′-lipoxygenase (5-LOX), 12′-lipoxygenase (12-LOX) and cyclooxygenase (COX) pathways, which are responsible for the production of eicosanoids, which include leukotrienes and prostaglandins (Halpern 2000;Whitehouse et al. 1997;Whitehouse and Rainsford 2006). PCSO-524® also elicits an anti-allergic and anti-inflammatory action by controlling the 5-LOX pathway. This regulates both the inflammatory and immune responses, specifically the allergic response induced by interleukin-4 (IL-4) (Dugas 2000). The safety and efficacy of the extract PCSO-524® have been examined in adult, child and adolescent populations, with positive effects in terms of reduced asthmatic (Lello et al. 2012;Emelyanov et al. 2002;Mickleborough et al. 2013), inflammatory bowel disease (Tenikoff et al. 2005) and osteoarthritis symptoms (Zawadzki et al. 2013). With respect to the potential application for symptoms of inattention, impulsivity or hyperactivity, the anti-inflammatory actions of PCSO-524® may benefit children who have clinical or subclinical ADHD by decreasing the ratio of arachidonic acid (pro-inflammatory) to eicosapentaenoic acid (anti-inflammatory) (AA/EPA), which may lead to improvements in associated symptoms (Young et al. 2005;Sorgi et al. 2007).
In the current randomised controlled trial, we investigated the effects of PCSO-524® on the symptoms of hyperactivity, impulsivity, inattention and cognition. The primary aim of the current study was to determine if supplementation with PCSO-524® reduced parental reports of hyperactivity and inattention in a population of Australian children and adolescents aged 6 to 14 who had increased levels of hyperactivity or inattention compared with placebo. A secondary aim of the study was to investigate changes in objective computerised measures of cognition and mood as well as electrophysiological measures of brain wave ratios.

Overview
The study was a 14-week randomised, double-blind, placebo-controlled, two-arm, parallel group clinical trial for which children and adolescents were randomised to receive either three capsules (≤45 kg) or four capsules (>45 kg) of PCSO-524® or a matching placebo. This study was approved by the Swinburne University Human Research Ethics Committee (project 2010/175) and was registered with the Australian New Zealand Clinical Trials Registry (ANZCTRN12610000978066). All procedures were conducted in accordance with the Declaration of World Medical Association (2008) and good clinical practice (GCP) guidelines. For an expanded discussion on the methods for this study, see Kean et al. (2013).

Study aims and hypotheses
The primary aim of the study was to examine the effect of 14 weeks of administration of PCSO-524® on levels of hyperactivity, impulsivity and inattention in children aged 6 to 14 years compared with placebo. The primary outcome was the Conners Parent Rating Scales (CPRS), a comprehensive checklist for acquiring parental reports of the behavioural problems that presented, which was completed every 4 weeks (Conners et al. 1998). Secondary outcomes investigated cognitive changes by the use of the Test of Variables of Attention (TOVA; Greenberg and Waldman 1993) and the Computerised Mental Performance Assessment System (COMPASS; Scholey et al. 2010). Changes in mood were assessed by the use of the Brunel Mood Scale (BRUMS) for adolescents (Terry et al. 1999). These mood scales were completed by the parents independently or (more usually) with the child. The acquisition of resting-state electroencephalography (EEG) was based on previous research into the differentiation of theta/beta and theta/ alpha ratios from non-ADHD children (Mann et al. 1992) as well as between subtypes (Clarke et al. 2001). This was conducted in two states: eyes open and eyes closed. For the sake of brevity, the EEG results are reported elsewhere.

Participants and trial site
One hundred and forty-four children aged between 6 and 14 years were recruited for the study and initially allocated to either a PCSO-524® or placebo group. All cognitive and electrophysiological testing took place within the Swinburne Centre for Human Psychopharmacology at Swinburne University, in Victoria, Australia. Parents completed additional CPRS forms at home during weeks 4, 10 and 18 (4 weeks post-treatment). Families that were based interstate (distant participants) completed all CPRS, BRUMS and symptom checklists in their home environments, at scheduled time points.

Inclusion criteria
Inclusion criteria were as follows: healthy, non-smoking males and females aged between 6 and 14 years, who had Diagnostic and Statistical Manual of Mental Disorders Fourth Edition (DSM-IV) ADHD rating score of greater than 15, who were fluent in English, had parental or legal guardian consent and verbal consent from the child. The DSM-IV rating scale is a reliable and valid four-point (0 = never or rarely, 1 = sometimes, 2 = often, 3 = very often) 18-item semi-structured interview that assesses symptom severity (Faries et al. 2001). A score of 15 points or higher on the DSM-IV rating scale allowed investigators to establish that participants had elevated levels of hyperactivity, inattention or both. Criteria for ADHD subtypes (inattentive or hyperactive-impulsive), requires six or more scores in the higher range (2 = often, 3 = very often) of the scale for that subtypes (DuPaul et al. 1998).

Exclusion criteria
Exclusion criteria were as follows: primary medical diagnosis other than ADHD, oppositional defiant disorder or similar behavioural disorders; currently taking any medication (other than stimulants if a formal diagnosis of ADHD or other behavioural disorder has been made); current or history of heart disease, or high blood pressure, or diabetes; health conditions that would affect food metabolism, including the following: food allergies, kidney disease, liver disease and/or gastrointestinal diseases (e.g. irritable bowel syndrome, coeliac disease, peptic ulcers); pregnant or breast feeding; unable to participate in all scheduled visits, treatment plan, tests and other trial procedures according to the protocol; allergy to shellfish; epilepsy or photosensitivity.

Randomisation and treatment schedule
Participants were allocated randomly to coded treatment groups. All participants were assigned to treatment groups A or B through the use of a computer-generated random number, which was done by a neutral third party. Blinding was achieved by enlisting a person outside of the project to code the treatments and maintain the key to this code until data collection was completed. An emergency code break envelope was provided to the principal investigator, which was to be opened only in case of emergency. The schedule for testing is presented in Table 1. Weight plays a large role in the digestion and absorption of nutrients, so weight was used to determine how many capsules each child would be required to consume (≤45 kg = 3 capsules; >45 kg = 4 capsules).

Procedure
Parents who were interested on behalf of their children contacted the university via telephone or e-mail and underwent a telephone screen to determine the eligibility of their child. Eligible participants who lived interstate were provided with hard copies of consent forms and were enrolled in the study after signed hard copy consent forms had been returned. Eligible children attended the university and completed consent on-site on the practice day. All families underwent a practice day information session or telephone call during which they completed screening questionnaires and familiarised themselves with the study procedures and tests. Local (on-site) participants underwent a baseline session during which they completed all tests (which included an EEG) and were allocated randomly to receive one of the two treatments (PCSO-524®/placebo). They were required to take either three or four capsules daily (in the morning, with breakfast) for 14 weeks. The outcome measures, including the primary outcome, the CPRS, were completed by parents during weeks 2 (baseline), 4, 8, 10, 14 and 18 (Conners et al. 1998).
Families that lived interstate were mailed a study kit that contained the participant's randomised treatment and were contacted to complete their study forms on specific dates, after their baseline start date. These participants did not complete cognitive assessments. Remaining testing sessions followed the schedule detailed in Table 1. Participants completed a treatment compliance diary and marked each day when their child consumed their treatment. At the end of the study, participants were required to return the compliance diary as well as any remaining treatment. A follow-up telephone call was undertaken at the conclusion of the study to enquire about compliance rates for each participant. All parents completed a food frequency questionnaire on behalf of the child at the start of the study and then a subsequent 7-day food diary during week 4 to determine if the child's omega-3 intake from non-trial sources remained consistent throughout the trial. Parents also completed the CPRS and the BRUMS 4 weeks following the cessation of the active or placebo administration (week 18) to determine if there were any changes in mood or behaviour. Parents completed a symptom checklist to monitor for any adverse events. Parents were questioned about any non-specific adverse events (AEs) at each scheduled time point (weeks 4, 8, 10, 14 and 18).

Outcome measures
The a priori primary outcome was the CPRS (Conners et al. 1998). Secondary outcomes included the computerised COMPASS cognitive battery, designed to allow assessment across the major cognitive domains, i.e. attention, working memory, secondary memory and executive function (Scholey et al. 2010). The following cognitive COMPASS tasks were administered: word presentation, immediate word recall, picture presentation, simple reaction time, choice reaction time, numeric working memory, delayed word recall, delayed word recognition and delayed picture recognition. Each outcome was scored in terms of accuracy and speed of response. The TOVA was employed to objectively assess symptoms of inattention and impulsivity. The TOVA is a computer-based assessment of inattention (Greenberg and Waldman 1993). It is considered to be a gold standard test for ADHD and associated symptoms (Llorente et al. 2008). The test has two modes divided into four quarters. The target infrequent mode in quarters 1 and 2 (36 targets; 126 nontargets) is the traditional form used to measure vigilance. This is followed by the target frequent mode in quarters 3 and 4 (126 targets; 36 non-targets) that denoted the high inhibition demand mode. The BRUMS, known formerly as the Profile of Mood States-Adolescents (POMS-A), was used to assess the mood states of the children. The BRUMS contained 24 simple mood descriptors such as angry, energetic, nervous and unhappy. It was designed specifically for adolescent populations, and the validation process can be found in Terry et al. (1999).
The Global Clinical Impression (GCI) scale was used to measure illness severity, global improvement or change and therapeutic response. The GCI is rated on a seven-point scale, with the severity of illness scale using a range of responses from one (normal) through to seven (among the most severely ill patients (Busner and Targum 2007)). Parents also completed a Food Frequency Questionnaire (FFQ) that provided a detailed description of the child's food intake. During week 4 of the trial, parents completed a 7-day food diary that recorded what the child ate for breakfast, lunch and dinner, as well as any significant snacks throughout those days. This diary was compared with the FFQ at baseline to determine any changes in diet that may have occurred during the study. Resting EEG and steady-state topography (SST) were recorded in on-site participants; however, the results due to their size are not reported here.

Statistical analysis
Analysis was performed using the Statistical Package for the Social Sciences (SPSS v.20). Repeated measures analysis of variance (ANOVA) were applied to all data, and in each analysis, diagnosis and medication were included as categorical covariates. Analysis of covariance (ANCOVA) was applied to all cognitive data to control for any confounding variables that may have impacted the performance on the measures. A multiple comparison test (Bonferroni) was applied to all data, which included all exploratory subsample analyses and the effect sizes (Cohen's d) provided. Cohen's effect sizes are interpreted as 0.2 = small, 0.5 = medium and 0.8 = a large effect size. Significance level was set at a p < 0.05. Intentionto-treat analysis, which used the last observation carried forward, was applied to any missing data from endpoint analysis, which included any eligible data from participants who dropped out.

Participant characteristics
Three hundred and fifty-one participants were screened initially for the trial, which resulted in 144 participants who met inclusion criteria and who were randomised to receive either PCSO-524® or a placebo for 14 weeks. The mean age was 8.7 years (SD = 2.24) of whom 123 were male and 21 were female (see Table 2 for demographic data). In total, the data of 112 participants were included for final analysis through the use of intention-to-treat analysis, with the final observation carried forward. Following random assignment and dropouts, there was a significant demographic difference in the number of females in the PCSO-524® group compared to the placebo group (N = 17; PCSO-524® n = 14). To further explore this, a repeated measure analysis was conducted investigating the effect of treatment on male and females only. This data is reported further on.
Whole sample treatment group differences were noted in secondary outcome, the COMPASS cognitive task, which included significant improvements in working memory on three domains in favour of PCSO-524®. No further treatment group differences were found in an analysis of the entire sample on remaining primary and secondary outcomes.
As the sample population included children taking pharmaceutical medications, careful interpretation of the analysis and outcomes was needed. Each result was analysed using diagnosis and medication status as a covariate. Any statistical influence of these covariates on any outcome is noted in the relevant analysis. These participant numbers are broken down by groups including on-site testing, distant testing and gender as well as to which treatment group these participants were randomised (see Table 3 and Fig. 1).

Conners Parent Rating Scale
A baseline summary of the CPRS data was included investigating differences between demographic data (see Table 4). Comparisons between gender, medication status, diagnosis status and testing location were conducted using paired sample t test. Any significant outcome was further investigated using Wilcoxon signed-rank test for non-parametric datasets. There were significant baseline differences for the peer relations (p < 0.05), oppositional defiant disorder (p < 0.01), impaired relationships (p < 0.01) and impaired home life (p < 0.01) outcomes between the diagnosed and nondiagnosed groups as well as significant differences on the peer relations (p < 0.05) and impaired relationships (p < 0.05) between medicated and non-medicated groups. No further differences were noted in any other demographic data at baseline. Despite these demographic differences, following randomisation, there were no significant differences between treatment groups on any of the CPRS outcomes.
Analysis of the primary outcome found no significant differences between treatment groups on CPRS following 14week supplementation. To further understand potential treatment effects, we also conducted post hoc analyses with subgroups. This was done to better understand whether there was a treatment effect related to the severity of the symptoms displayed by children and adolescents. Subsample analysis indicated significant treatment effects for participants who had less-severe symptoms (high inattention, high hyperactivity or no subtype-non-combined type) than those who had more severe symptoms (combined high hyperactivity and inattention-combined type) based on attention-deficit/hyperactivity disorder IV rating scale scores (DuPaul et al. 1998).
Non-combined type (NCT) (N = 43; PCSO-524® n = 23) analysis revealed a significant improvement in children who consumed PCSO-524® on CPRS scores of hyperactivity, learning abilities and improved behaviour at home, as well as improvements on DSM scores of attention and hyperactivity. Furthermore, there was a significant reduction in ADHD probability ratings in children who consumed PSCO-524® compared with placebo; this result was associated with a main effect for diagnosis (p = 0.03) (see Table 5). No baseline differences were seen in the NCT sample. There were also no significant differences between treatment groups in terms of those with high inattention (p = 0.08) or high hyperactivity (p = 0.10) at baseline. The NCT subsample was comprised of three classifications, which included those who met criteria for high inattention, those who met criteria for high hyperactivity and those who did not meet criteria for either high inattention or high hyperactivity, denoted from here as non-subtype group. Further analysis of these groups found that those children taking PCSO-524® and displaying high inattention (N = 19; PCSO-524® n = 11) improved in parental ratings of executive function (p = 0.01; d = 0.38), aggression (p = 0.01; d = 0.70), conduct (p < 0.01; d = 1.03) and oppositional defiance (p = 0.04; d = 0.46). Improvements in aggression and oppositional defiance were associated with main effects for medication (p < 0.05) and diagnosis (p < 0.05). This may indicate the potential for PCSO-524® as an adjunct therapy in ADHD-diagnosed children displaying specific issues with attention; however, future studies are needed to establish this association. Improvements in executive function were also associated with a main effect for diagnosis (p < 0.05). There were no improvements on any domain for those children displaying symptoms of high hyperactivity (N = 10; PCSO-524® n = 4) or the non-subtype group (N = 15, PCSO-524® n = 9). Only a single baseline difference was noted in the conduct issues outcome for the non-subtype group (p < 0.05).
Group analysis of the combined type (CT) (N = 65; PCSO-524® n = 29) revealed an improvement in attention, executive function and DSM ratings of attention in the placebo group (see Table 6); however, both symptoms of executive function and DSM ratings of attention showed a significant main effect for medication status, which highlights the possible influence of pharmaceutical treatment on parental ratings of participant's behaviour. No baseline differences were noted in the CT sample.

Clinical Global Impression scale
There were no significant differences between treatment groups at baseline or during the following 14 weeks of treatment.

Computerised Mental Performance Assessment System
There were no significant differences between groups at baseline for any of the cognitive measures. Results of the reaction time cognitive data did not include fast guesses (<200 ms). Whole sample analysis (N = 85) revealed significant effects in favour of PCSO-524® between baseline and week 8. ANCOVA analysis was conducted using diagnosed and medication status as well as baseline scores as covariates. The main outcomes were improved memory accuracy scores of participants in the PCSO-524® group when they recalled target (p = 0.05, d = 0.48) and non-target (p = 0.02, d = 0.56) pictures correctly; this is complemented by significant overall picture recognition accuracy (p = 0.02, d = 0.56) (see Fig. 2ac). There were no main effects for diagnosis or medication on any of the whole sample outcomes.

COMPASS subsample analysis
Baseline differences (p < 0.05) were noted between treatment groups on three subscores for the word recognition task in the diagnosed (Ds) (n = 28; PCSO-524® n = 14) subsample only. A single significant difference was noted on the delayed word recall task in the non-diagnosed (NDs) (n = 57; PCSO-524® n = 31) subsample (p < 0.05). No other baseline differences were noted in either subsample. There were no main effects for medication in the diagnosed subsample on any COMPASS outcome.
In the NDs subsample, significant improvements were seen on the same working memory scores as in the whole sample analysis. ANCOVA analysis of the subsample revealed that those in the PCSO-524® group had improved picture recognition (p = 0.03, d = 0.67), correct target picture recognition (p = 0.05, d = 0.58) and correct non-target picture recognition (p = 0.02, d = 0.69). In the Ds subsample, a significant improvement in numeric working memory (Fig. 3) was demonstrated in those taking PCSO-524® between baseline and week 8 (p = 0.02, d = 0.92).

Test of Variables of Attention
The TOVA is a highly demanding task that requires significant sustained attention during a long period of time. Analysis was conducted using a 2 × 2 repeated measures ANOVA (quarter × time) on each mode (target-infrequent [first half] mode or target-frequent [second half]) mode to evaluate the individual influences of each quarter on the data. Any quarter that had excessive omission errors (>90%) was considered invalid. Participants whose data was deemed invalid were removed from the final analysis. Seventy-seven participant datasets were included in the final analysis. There were no significant differences between groups at baseline. No statistically significant differences were found between the treatment groups in whole sample analysis.

Brunel Mood Scales (BRUMS)
The data of 108 participants were used for analysis. There were no significant differences between treatment groups at baseline. Whole sample analysis revealed significant changes in feelings of fatigue and confusion. Fatigue increased in the PCSO-524® group until week 8 (p = 0.02, d = 0.49), when it levelled off at week 14 (p = 0.01, d = 0.53), whereas there was a consistent decrease in fatigue in those children in the placebo group. Both treatment groups had reduced feelings of confusion during the 14 weeks; however, the placebo group did so more significantly at week 8 (p = 0.02, d = 0.46) and at week 14 (p = 0.01, d = 0.55).

BRUMS subsample analysis
In the Ds subsample, there was a significant decrease in feelings of confusion in the placebo group at week 8 (p = 0.00, d = 1.14) and at week 14 (

Sex differences
To evaluate the difference between gender on behavioural outcomes, a repeated measure ANOVA was conducted on males in the study. An analysis on females was not conducted due to the low number of females at the conclusion of the study coupled with the difference in numbers between groups. A repeated measures ANOVA using Bonferroni multiple comparison analysis was conducted on the male-only cohort. There were no significant differences between treatment groups on any CPRS domains following a whole sample analysis (  ADHD probability scores were associated with main effects for diagnosis and medication status.

Adverse effects
There was a significant difference between groups in 'feeling colder' at baseline; however, this difference dissipated at the end of the study. No other differences in symptoms were noted at baseline or during the course of the study (see Table 9).

Post-treatment follow-up
Week 18 final behaviour (CPRS) and mood (BRUMS) follow-up compliance completion was low (53%). Paired sample t test indicated a significant worsening in executive function for the placebo group in the CT condition (p = 0.05; d = −0.34). There was a significant increase in tension (p = 0.05, d = 0.36) throughout the whole sample in the PCSO-524® group, following cessation of treatment. In subsample analysis, those children who had been diagnosed with ADHD and who had finished taking PCSO-524® showed increased tension (p = 0.05, d = 0.51) and symptoms of depression (p = 0.02, d = 0.58). These results should be interpreted with caution due to the low completion rate.

Dropouts, withdrawals and compliance
There were 24 participant dropouts. The majority of dropouts occurred due to family issues (n = 9 placebo; PCSO-524® n = 4) that left the participant unable to complete testing procedures (see Fig. 1 for a study recruitment flowchart). A common issue with younger participants was an inability to swallow the capsules (placebo n = 7; PCSO-524® n = 4). Some participants began ADHD medication or made adjustments to their medication regimen (placebo n = 3; PCSO-524® n = 2) and were excluded from further testing. Two participants withdrew from the study due to symptoms; one participant complained of an increase in noise sensitivity (placebo), and one parent reported an increase in the child's Correct Non-Target Picture RecogniƟon Fig. 2 Graphs showing accuracy percentage on picture recognition accuracy, accuracy for correct target picture recognition and accuracy for correct non-target picture recognition hyperactivity while at home (PCSO-524®). Two further participants did not comply with treatment and testing protocols and were withdrawn from the analysis (PCSO-524® n = 2). Ten interstate participants were lost to follow-up and failed to return any data (PCSO-524® n = 5). Compliance rates were determined via a pill count, the compliance diary and a follow-up telephone call. The mean recorded compliance rates with the single daily intake were 96.67%. There were no significant differences between compliance, diagnosis and ADHD medication between the treatment groups.

Discussion
The results of this study did not support the hypothesis that PCSO-524® would improve parent-rated levels of hyperactivity, inattention and impulsivity in children with high levels of behavioural problems aged 6 to 14 years over placebo. Despite this, several positive findings in exploratory subgroup analyses revealed specific benefits of PCSO-524® in ameliorating the symptoms of hyperactivity and inattention in children who did not meet criteria for parental ratings of combined hyperactivity and inattention. In addition, those children consuming PCSO-524® demonstrated significant memory improvements on cognitive tasks against placebo, regardless of symptom severity. The present study utilised parents' ratings to understand how the symptoms present in each child affected their behaviour at home and at school, regardless of their clinical diagnosis. This type of exploratory subsample analysis allows researchers to understand the benefits of unique treatments such as PCSO-524® and has been utilised previously in rigorous intervention trials in child and adolescent populations (Manor et al. 2012). Significant improvements in hyperactivity, inattention and learning in the NCT group highlight the possibility of mineral deficiencies in the subsample population under study. This is consistent with previous research indicating parental ratings of children's behaviour correlates with mineral deficiencies (Sinn et al. 2008) as well as prevalence rates of ADHD (Visser et al. 2013). This also highlights the possibility for future dose range investigations of this extract (PCSO-524®) in order to determine whether children classified as combined type require a larger dose of the intervention or if unrelated factors lead to the lack of improvement in behavioural ratings. The Conners Rating Scales are among the most validated test instruments used in research on ADHD and subclinical levels of ADHD (Archer and Newsom 2000). Hurtig et al. (2007) investigated the presence and severity of ADHD symptoms in a cohort of Finnish children and adolescents in whom ADHD had been diagnosed. Findings indicated that those children who had a combined subtype diagnosis had significantly worse problems of inattention, compared with children who had the inattentive subtype alone. This may indicate as to why the NCT children in the current trial benefited more from the treatment intervention than those who had a CT outcome. Furthermore, children within the NCT subsample who displayed levels of high inattention only demonstrated greater benefit due to the intervention than those  . 3 Effects of PCSO-524® (Lyprinol/Omega-XL) on the numeric working memory outcomes. Graphs depict mean COMPASS reaction time scores with SD at baseline, week 8 and week 14 compared to placebo. Significant differences (*p < 0.05) are indicated; Cohen's d effect sizes are provided children classified as high hyperactivity only or non-subtype. This outcome supports a previous study by Johnson et al. (2009) that demonstrated that responders to a combination of lipids were more likely to be male and have a diagnosis of ADHD inattentive subtype. Gillies et al. (2012) concluded that combined omega-3 and omega-6 PUFA supplements may be more likely to improve ADHD-associated symptoms than omega-3 supplementation alone. Previous biochemical reports have highlighted that the potent anti-inflammatory properties of P. canaliculus may be due to the synergistic action of multiple PUFAs, rather than the omega-3 (DHA) content by itself (Treschow et al. 2007).
The significant improvements in whole sample COMPASS scores on measures of picture recognition indicate that PCSO-  In clinical and subclinical domains, externalised symptoms such as hyperactivity and inattention require accurate measures by which research can detect changes reliably. Continuous performance tests (CPTs) offer crucial insight into issues with inattention and impulsivity. Greenberg and Waldman (1993) established the normative data for the TOVA (formerly the Minnesota Computer Assessment; Greenberg 1987) in a 1993 publication that used data collected from 775 children aged 6 to 16. In the present study, the results from the TOVA testing assessed the accuracy and speed at which participants recovered after they made an error, which was denoted as post commission. In a consistent trend, children without ADHD who consumed PCSO-524® demonstrated an improved ability to respond faster to targets consistently following an incorrect response. A previous study by Vaisman et al. (2008) suggested that interventions with greater EPA/DHA ratios, even in subgram amounts, could impact the visual sustained attention performance in paediatric populations. Despite promising subsample outcomes, whole sample analysis of the TOVA results did not demonstrate significant improvements in either treatment group.
Neurochemical explanations for improvements in hyperactivity, impulsivity and inattention remain difficult to discern. In a study by Ma et al. (2011), researchers established that children who were classified to have ADHD hyperactive/impulsive type had significantly lower cortisol levels than the ADHD combined type and ADHD inattentive-type children. As PCSO-524® is an effective mediator for the 5-LOX and 12-LOX pathways, its presence in this study may have improved levels of cortisol via its effect on cytokines. Cytokines play a key role in the hypothalamic-pituitary-adrenal (HPA) axis and have been shown to be involved in cognitive processes, stress and depression (Wilson et al. 2002). Lower levels of omega-3 (DHA) within the adult brain have been shown to dysregulate the functions of the HPA axis (Vaisman et al. 2008). Lipid profile studies have demonstrated a higher AA/EPA ratio in those children with ADHD (Stonehouse et al. 2013). Improvements in this AA/EPA status has correlated with a decrease in ADHD-associated symptoms (Stonehouse et al. 2013). Germano et al. (2007) evaluated the effects of LC PUFAs in ADHD children against normal controls highlighting a significant correlation between improved clinical symptoms of hyperactivity and inattention with reduced disparity in the AA/EPA ratio. Despite the lower level of omega-3s in PCSO-524®, previous evidence of its ability to block pro-inflammatory pathways highlights a possible mechanism of action via returning the AA/EPA ratio or inflammatory/antiinflammatory ratio to a more balanced state (Halpern 2000;Lello et al. 2012;Gillies et al. 2012). LC PUFAs improve blood lipid profiles, cardiovascular health, cell membrane fluidity and cell signalling cascades, so the introduction of any amount of LC PUFAs into a cell system that has low levels of it may improve behaviours which are affected by its absence. The most prevalent fatty acid within the brain is DHA, which constitutes 45 to 65% of fatty acids in nervous tissues (Ma et al. 2011). There were no significant differences in compliance, omega-3 intake through food or reporting of symptoms between treatment groups, and these results may indicate an increased bioavailability of omega-3s in the brain following PCSO-524® supplementation.
Limitations of the current study include the broad spectrum of participants. Despite the large sample size, the variety in participant demographics, diagnostic status and locations may hinder the extrapolation of the results. Although it does not directly impact the outcome of the data collected, the small number of participants' families who failed to provide medication information diminishes any inferences that could be made regarding medicationspecific adjunct treatment. The significant number of the males in the study may highlight a well-known bias in the wider population; males with ADHD tend to exhibit more externalising behaviours such as hyperactivity, inattention and impulsivity, whereas females with ADHD tend to exhibit more social issues and internalising comorbidities (anxiety) (Gershon and Gershon 2002;Carlson et al. 1997). This bias may impact the reasons parents decide to join the study as well as explain the greater number of males over females recruited. The outcome of the maleonly analysis indicates that PCSO-524® may be beneficial for young males with issues of hyperactivity or inattention at the subclinical level. Further investigations need to be done to verify this outcome.

Conclusions
The primary outcome of the current study was not supported by the results. Replicated randomised trials with dose variations and lipid profiling are needed to understand the neurological and behavioural benefits of PCSO-524® in this population. Despite this, exploratory post hoc analysis of the primary outcome of the current study indicates promise for the use of PCSO-524® in the treatment of symptoms of inattention, hyperactivity and impulsivity in children and adolescents with or without ADHD displaying less severe behavioural symptoms. PCSO-524® also indicated an ability to improve working memory in children and adolescents with increased levels of hyperactivity, inattention and impulsivity. Post hoc analysis of those children with a diagnosis of ADHD also demonstrated improved inhibition and reduced error making compared to placebo. The use of PCSO-524® as an adjunctive treatment should be subjected to additional clinical trials (i.e. together with stimulant medication). Further large-scale RCTs should be conducted administering PCSO-524® to children and adolescents with subclinical levels of hyperactivity inattention and impulsivity. Future neuroimaging trials may assist in elucidating the mechanism of action.

Clinical significance
PCSO-524®, a marine-based LC PUFA, may be a safe alternative to standard pharmaceutical treatments for children and adolescents with ADHD who have less severe levels of hyperactivity, inattention and impulsivity.