Cancer Stem Cells: Cellular Plasticity, Niche, and its Clinical Relevance

Cancer handles an estimated 7.6 million deaths worldwide per annum. A recent theory focuses on the role Cancer Stem Cells (CSCs) in driving tumorigenesis and disease progression. This theory hypothesizes that a population of the tumor cell with similar functional and phenotypic characteristics as normal tissue stem cells are responsible for formation and advancement of many human cancers. The CSCs subpopulation can differentiate into non-CSC tumor cells and promote phenotypic and functional heterogeneity within the tumor. The presence of CSCs has been reported in a number of human cancers including blood, breast, brain, colon, lung, pancreas prostate and liver. Although the origin of CSCs remains a mystery, recent reports suggest that the phenotypic characteristics of CSCs may be plastic and are influenced by the microenvironment specific for the individual tumor. Such factors unique to each tumor preserve the dynamic balance between CSCs to non-CSCs cell fate, as well as maintain the proper equilibrium. Alternating such equilibrium via dedifferentiation can result in aggressiveness, as CSCs are considered to be more resistant to the conventional cancer treatments of chemotherapy and radiation. Understanding how the tumoral microenvironment affects the plasticity driven CSC niche will be critical for developing a more effective treatment for cancer by eliminating its aggressive and recurring nature that now is believed to be perpetuated by CSCs.


Introduction
Multicellular organisms require a poised homeostatic equilibrium between cellular proliferation and differentiation for development and growth of the individual. Disruption of this equilibrium causes the devastating consequences of malignancy. A recently proposed hierarchical model of tumorigenesis postulates that the uncontrolled expansion of many cancers is predominantly driven by a rare subset of cells within the tumor population known as Cancer Stem Cells (CSCs) [1,2]. Although similar to somatic stem cells, CSCs have an This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. enhanced potential to self-renew, differentiate into non-stem cancer cells and promote intratumoral heterogeneity in order to sustain uncontrolled tumor growth. It has also been reported that CSCs have an innate ability to resist conventional multi-modality therapy and considered to be partly responsible for the high rate of disease recurrence and clinical relapse observed in many cancers [3,4]. Recent evidence suggests that therapeutic stress may also promote cellular plasticity, which mediates the conversion of normal cancer cells to a CSC-like state [5,6]. These newly converted stem-like cells possess enhanced tumor formation abilities and are more infiltrative than non-stem cancer cells in the animal model, adding to the attenuated therapeutic efficacy seen in clinical settings. The data argues against the unidirectional flow of cellular hierarchy, rather suggesting a bidirectional flow whose activation may be influenced by various factors within the tumor-specific microenvironment or "niche" [7,8]. The dynamic equilibrium between CSCs and their lineage-committed nonstem counterparts is partly regulated by the rate of differentiation and the balance between asymmetric and symmetric cell division in the CSC compartment. Because the heterogeneous tumor population contains a small number of CSCs amid the larger number of non-stem differentiated tumor cells, it is essential to understand the regulation of such equilibrium. Any shift in the equilibrium state will critically influence the clinical outcomes and lead to a more CSC-rich tumor, which will be more aggressive and produce poorer prognoses in patients [9,10]. By elucidating the various mechanisms for the maintenance of this equilibrium state and the relationship between Cancer Stem Cells and their niche, one can improve the current standard of care as well as develop targeted strategies that will enhance the therapeutic efficacies of anti-cancer therapies. In this recent review, we will summarize the recent finding of the mechanisms of the intratumoral cell fate equilibrium and the consequence of its regulation in disease progression, as well as discussing the potential development of therapeutic modalities that target CSCs.

The CSC Hypothesis and its Role in Disease Progression and Tumorigenesis
Whereas normal tissues display an ordered developmental structure underlying cellular heterogeneity, allowing various cell types to maintain the generation of stable differentiated progeny cells through epigenetic regulation, malignant tissues possess disorganized cellular programming that gives rise to heterogeneous cancer cell populations [11]. In attempt to explain the development of heterogeneous tumors, two competing theories have been proposed: the clonal evolution theory and the cancer stem cell theory. In 1976, Peter Nowell first proposed the clonal evolution model and introduced the idea that cancer was driven by the accumulation of somatic cell mutations [12,13]. This theory postulates that a single clone survives an oncogenic mutation that leads to a more aggressive phenotype, and upon proliferation, its daughter cells acquire additional somatic mutations that further promote survival fitness and aggressive behavior. The aggressive daughter cells continue to divide and eventually outnumber the non-aggressive populations because of their high fitness. In time, the clones acquire additional mutations, creating genetically diverse clonal populations. These clones evolve through what resembles Darwinian selection of survival of the fittest, in which only those clonal populations that can survive the accumulation of many mutations will comprise the final heterogeneous tumor [14]. According to the clonal evolution model, each cell within the tumor is considered to possess an equal potential to promote tumorigenesis, leading to the devastating outcomes of human malignancy. This concept is the foundation of a majority of the currently available cancer therapies, which are designed to target and eliminate all the cancer cells within a tumor with higher proliferative capacity to achieve cures. However, in many human malignancies, especially solid cancers, 'cures' remain relatively rare commodities and cancer-related death rates remain very high [15].
The more recently proposed hierarchical model of tumorigenesis claims that only a rare subset of cells, known as Cancer Stem Cells (CSCs), is responsible for the uninhibited tumorigenic capacity of malignant cancers. Although similar to normal stem cells, CSCs have an enhanced potential to self-renew, differentiate into non-stem cancer cells, and initiate new tumor formation by giving rise to heterogeneous cell populations [4,16,17]. In contrast to the clonal evolution model, multipotent characteristics of CSCs are considered to be responsible for intratumoral heterogeneity through their aberrant differentiation capacity [18]. The cells with the most tumorigenic potential exist at the top of a hierarchical organization that once closely resembled the ordered of developmental structure for normal tissues but has since become disordered [19]. These cells have also been shown to resist primary multi-modality therapies in some cancers, which contribute to the dismal prognoses in patients, with inevitable disease relapses [20,21]. According to the CSC model, the only way to prevent disease relapse and achieve durable therapeutic response is to eliminate the CSC population. While differentiated cancer cells do not have the ability to self-renew indefinitely and cannot produce cells of different origins, the enriched properties of CSCs allow the formation of diverse, heterogeneous tumor populations [22,23].
Melanoma is widely considered to strongly support the CSC model [24]. Initially, melanomas form flat lesions that can be removed by gross resection [25]. However, melanomas quickly progress to contain heterogeneous subpopulations that express different genes [26]. This property makes metastatic melanomas extremely difficult to treat because of their ability to mimic vasculature, a property that suggests the presence of nondifferentiated cells in the tumor population [27]. Furthermore, malignant melanomas that exhibit higher expression of stem cell markers correlate with poor prognoses [28] and resistance to the cytotoxic agent doxorubicin [29]. These therapies eradicate most of the tumor growth, but populations of resistant cells remains, which give rise to novel tumors that are chemoresistant and immunoevasive [30]. Because melanomas lack homogeneous clones with similar genomic profiles and instead exhibit a hierarchical structure of mature and progenitor cells, melanoma supports the CSC model. On the other hand, some malignant tumors in other tissues seemingly contradict the CSC theory. Retinoblastoma can occur after only two mutations [31], a property that supports the clonal evolution theory. Examination of the karyotypes of retinoblastoma cells showed that non-disjunction in chromosome 13 was to blame for this malignancy, further supporting the idea that sequential mutations in identical cells drive tumor development and progression [32]. As these cases exemplify, it is highly unlikely that one model of tumorigenesis is completely correct, but rather it is more likely that tumor development exhibits characteristics from both [33,34]. The two models can be used in conjunction to explain more effectively the basis of tumor heterogeneity, disease progression and recurrence.
Human cancers frequently display substantial heterogeneity with many phenotypic features such as cellular morphology, gene expression (including cell surface markers, growth factors, and hormonal receptors), metabolism, and angiogenic, immunogenic, and metastatic potentials [35]. The CSC model proposes that while every cell in the cancer population is genetically equal, individual subsets within the tumor possess internal clonal heterogeneity [34]. This model puts the most tumorigenic cells at the top of its proposed hierarchy. These cells can go through the asymmetric cellular division, which results in one differentiated less-tumorigenic or non-tumorigenic cancer cells, as well as inducing self-renewal of CSC daughter cells. It is these small CSC subpopulations that are believed to drive the initiation and expansion of the entire tumor, while non-CSCs, which constitute the majority of the tumor, contribute much less to tumorigenesis and growth, instead influencing the overall traits of established tumors ( Figure 1) [34].
One key factor that drives the regulation of tumor growth and heterogeneity is genetic instability, which results from an increased rate of cell proliferation in addition to mutations and epigenetic alterations [11]. If the cell cycle checkpoint fails to repair an error (i.e. additional or insufficient number of chromosomes or mutations) during replication, the cell will progress to the next step in the cycle, wreaking further havoc on genomic stability [36]. Both normal cells and cancer cells show spontaneous mutations and are usually targeted by DNA checkpoint and repair mechanisms to prevent the accumulation of aberrant cells. However, some mutations alter gene function in the DNA repair pathway and push cells to respond to the internal and external pressures from Darwinian selection [11]. The cells that thrive despite these genetic changes can evade DNA checkpoints and immunosurveillance to survive longer and eventually give rise to descendant cells hosting the same or added genomic abnormalities.

Cellular Plasticity
The proper development of a multicellular organism depends on the balanced equilibrium between differentiated cells committed to tissue lineages and cells with stem-like characteristics. The concept that CSCs and their more differentiated progeny exist in a dynamic equilibrium state has been recently proposed by many labs, including our own. These reports not only showed that CSCs can differentiate to possess a committed fate, but that non-stem-like cells can also acquire CSC-like state [6,9,19,37,38], which results in a dynamic relationship between the two populations. To maintain this intrinsic homeostatic state, a stable balance between the rates of CSC self-renewal, differentiation, and asymmetric division and the rates of interconversion between non-CSCs to CSCs must be maintained within the individual tumor [39]. One critical aspect of normal stem cell function and identity is asymmetric division, in which the cell creates descendants that retain the parental stemness characteristics as well as give rise to progeny cells that are committed to differentiation into multiple lineages [40]. The symmetrical division is also a fundamental feature of the tissue generation of normal adult stem cells in which two daughter cells preserve their stemness. These tightly controlled processes of cellular division are required to maintain dynamic balance and diversity among cell types and contribute to the global size of the tissue or organism (Figure 2). Any disruption of this process throws off the equilibrium ratio of number of stem cells to non-stem cells. The dedifferentiation of non-CSCs into CSCs has been attributed to cellular reprogramming during oncogenic transformations and initiates subsequent development of aggressive cancers [3,6,9,40,41]. These unprecedented rates of plasticity allow normal cancer cells to acquire stem-like states and in this way increase the intratumoral CSC frequency.
Normal developmental processes allow for the differentiation of progenitor cells, and because of cellular plasticity, these cells can adopt new and differentiated fates. Cellular plasticity is defined as the ability of adult tissue cells to undergo a dedifferentiation or differentiation process to adopt new phenotypic and functional identities [34,42,43]. During normal development, the change from stem cell to lineage-committed cell is a gradual process with phases that cause the cell to lose developmental potential until it reaches its final committed, differentiated state. At this point, we can see that stem cells exist in a dynamic equilibrium with their differentiated counterparts in a stable balance that is tightly regulated by various signaling pathways associated with microenvironments and external stimuli. When this balance is disturbed by transcriptional, epigenetic, or environmental changes, non-CSCs can undergo a dedifferentiation process to acquire stem-like characteristics and be reprogrammed towards a more aggressive tumorigenic fate [43]. Given the possible significance of this equilibrium process, next we will discuss various factors that can influence the intratumoral cell fate state.

The CSC Niche and Microenvironment in Disease Progression
Niches are pockets of distinct microenvironment with specific functional characteristics that form the habitat of certain cells with specific fates. Such microenvironmental pockets are regulated by a variety of factors and cell types, including immune cells, cancer-associated fibroblasts, extracellular matrix components, hypoxia, and pH [8,[43][44][45]. Cells found in the CSC niche are capable of maintaining or even acquiring stem-like characteristics to promote the fitness of each tumor [18,46,47]. A critical unanswered question is whether any cell that encounters this niche is capable of initiating the cellular reprogramming process to acquire a stem-like state or if only the cells with stem or progenitor cell state/lineage are capable of such reprogramming. In light of recent discoveries about cellular reprogramming both in the developmental as well as pathological context, it is conceivable that any cell in the presence of appropriate signals is capable of initiating the cellular reprogramming process to acquire the stem-like state. The example of fate reprogramming is also reported in the normal developmental setting, as certain progenitor populations demonstrate the ability to dedifferentiate to acquire stem-like characteristics. In Drosophila testes, a cluster of stromal cells known as spematogonial can dedifferentiate into germline stem cells to replace stem cell populations in the aging tissue [48]. This dedifferentiation process is also observed in the mouse testes [49]. In the hematopoietic system, PAX5 has been shown to be crucial in the differentiation process of lymphoid progenitor cells to mature B-cells [50]. A report showed that the deletion of PAX5 led to the dedifferentiation of a mature B-cell into a T-cell [51]. Thus, the elimination of cellular identity through a strong master regulator such as PAX5 can achieve reprogramming. The process of fate reprograming is also supported by landmark study published by the Yamanaka's lab in 2006, where they showed that reprogramming committed cells back to an induced pluripotent state required the stimulation of four transcription factors: Sox2, Oct4, Klf4, and c-Myc [15]. Authors showed that cells resting in a quiescent developmental state could be reprogrammed to become pluripotent progenitor cells by introduction of these factors. These four factors are involved in a poorly understood network comprised of other transcription factors, histone modification enzymes, and polycomb group complexes that collectively capable of reprograming of differentiated cells [52].
In the pathological condition, recent evidence widely supports the theory describing spontaneous and therapy-induced reprogramming of differentiated cancer cells to cancer stem-like cells. Iliopoulos et al. showed that IL6 was one of the key factors that mediated the conversion of non-CSCs to CSCs in breast cancer and prostate cancer [9]. They used a chemotherapy-induced model of oncogenesis to indicate that both non-CSCs and CSCs exist in a dynamic equilibrium in which, over many generations, the proportion of these two cell subpopulations remain continuous. A similar shift in the cell fate equilibrium is also present in Glioblastoma Multiforme (GBM), as differentiated glioma cells converted to glioma stemlike cells after exposure to clinically relevant doses of primary chemotherapy [6]. It is shown that this chemotherapy-induced cellular plasticity can enhance the Glioma Stem Cells (GSC) subpopulation in recurrent tumors and may be responsible for a more aggressive tumor phenotype [3,21]. During disease progression, the tumor-specific niche creates a dynamic equilibrium between the CSCs and their fate committed counterparts [2,9,53]. Any shift in this equilibrium state is regulated by the intratumoral microenvironment and can potentially influence the clinical outcomes of tumors since CSCs possess the intrinsic ability to resist conventional therapies (Figure 3). For the majority of solid cancers, the molecular mechanism of how this equilibrium is maintained with respect to a tumor-specific CSC niche continues to be poorly understood.
One proposed explanation for the maintenance of the CSC population is the Epithelial-to-Mesenchymal Transition (EMT) [54]. While this process is a key step in metastatic progression, it is also essential in development [55]. Recently, embryonic transcription factors have been shown to drive classic cancer traits, including apoptosis resistance [56] invasiveness [57] and motility [58]. The basal-like mammary cells in breast cancer have the ability to dedifferentiate spontaneously into a stem-like state post oncogenic transformation via EMT [10]. In GBM, CD95 has been shown to assist in the maintenance of EMT programming and to provide stem cell properties to glioma cells [59,60]. Overexpression of the transcription factor Twist, which is known to promote EMT, resulted in increased invasive potential and therapeutic resistance in two PDX glioma lines [61]. Notably, in GBM Twist did not induce the E-cadherin to N-cadherin switch that is observed in EMT in other tissues [61], suggesting that this factor promotes maintenance of stem cells in the brain rather than driving classical EMT.
In addition to tumor population hijacking properties that induce EMT in development to maintain CSC populations, the tumor microenvironment also promote the maintenance of CSCs. Because of their inability to access oxygen-containing vasculatures, tumors are more hypoxic than the surrounding healthy tissues [18]. It has been reported that hypoxia and intratumoral pH can promote a stemness niche by enriching an environment that supports the self-renewal capacity of stem cells through activation of various stemness associated genes and initiates dedifferentiation of non-CSCs [43,44,62,63]. Cellular response to hypoxia is commonly regulated by hypoxia inducible factors (HIFs), which are key transcriptional factors that are upregulated upon exposure to low-oxygenated conditions [64]. It has long been known that anti-cancer therapy can change the tumor microenvironment and induce therapeutic resistance in cells residing within this region [7,8]. We reported that anti-cancer therapy can induce these hypoxia-like responses to dedifferentiate non-CSCs to their stem-like state, and newly converted CSCs overexpressed both HIF1α and HIF2α [6]. Anti-glioma chemotherapy significantly enhances the number of intratumoral hypoxic foci in an orthotopic xenograft model and shifts the stemness equilibrium towards a more stem-like state. Thus, it is conceivable that hypoxia induced CSC niche may shift the intratumoral balance between CSCs and non-CSCs towards a more stem-like state, which in turn contributes to therapeutic resistance. The precise mechanism of hypoxia-induced CSC niche and its contribution to promoting therapeutic resistance still requires further investigation.
Hypoxic conditions also stimulate angiogenesis by inducing the release of angiogenic factors, including vascular endothelial growth factor (VEGF). Although HIF1α is often considered the master regulator of angiogenesis in hypoxia, HIF2α drives angiogenesis by regulating VEGF. VEGF has become one of the most well-known endothelial cytokines modulated by hypoxia, and its contribution to angiogenesis and tumor malignancy has been studied in various tumor models [65][66][67]. Angiogenesis and the production of a vascular network are essential for tumor and disease progression, and Stockmann et al. showed that VEGF was crucial for the formation of this vasculature. The perivascular niche that promotes maintenance of CSCs has been well documented. In the PDGFR-induced glioma model, nitric oxide-mediated expression of Nestin, Notch, and NO leads to stem-like characteristics in glioma cells, which enhance tumorigenic capacities in vivo [68]. The perivascular niche also induced VEGF expression, and a recent study has demonstrated that autocrine signaling of VEGF-VEGFR2 can promote GSC viability and tumor growth [69]. In a mouse model of skin cancer, efficient blocking of VEGFR2-neuropilin signaling can effectively deplete the CSC population [70]. Collectively, these studies point towards a notion that targeting the perivascular niche of CSCs by blocking VEGF-VEGFR2 signaling might be an effective CSC targeting strategy. Inhibition of VEGF function by using Bevacizumab, a humanized monoclonal antibody against VEGFA, has received accelerated approval to target tumor angiogenesis in glioma patients [71]. However, despite improving the quality of the patients' lives, nearly all patients with GBM progress, and Bevacizumab has been shown to enhance the dissemination characteristic in GBM [72]. Schnegg et al. recently demonstrated that VEGF-A inhibitors promote HIF1α-mediated expansion of the CSC population in melanoma, elegantly highlighting the role of therapy adaptive resistance mechanisms driven by the therapeutic stress induced selection pressure [73].
Angiogenesis can also be a result of a microenvironment with low pH, which has also been reported to contribute to the maintenance of the CSC niche [44]. For many cancers, extracellular pH levels are significantly more acidic than in normal tissues and are indirectly correlated to tumor size [44,74,75]. A shift to an acidic pH within the intratumoral microenvironment increases the expression of cancer stem cell markers and promotes the equilibrium to move towards stemness. CSCs exposed to an enriched therapy-induced stem cell niche will further increase cell proliferation, angiogenesis, immunosuppression, and chemoresistance [44,[74][75][76][77], which contribute to the poor prognoses of many cancers. Such plasticity-mediated adaptability may be critical for cancer cells to overcome targeted anticancer therapies and promote therapeutic resistance. Elucidating the molecular mechanisms that govern cellular plasticity will allow the development of effective targeting strategies to eliminate newly developed CSCs.

Clinical Implications of Cancer Stem Cells
One of the most difficult endeavors in the cancer stem cell field is to understand their contribution in the clinical setting. It is known that CSCs appear to be more resistant to conventional therapies such as radiation and chemotherapy than normal cancer cells because of their quiescence, or dormancy [78,79]. Tumor quiescence is a state of remission in which cancer cells are resting and undetectable for a period. This resting phase is commonly seen in patients who have endured constant multi-modal therapies such as radiation and chemotherapy [48,80,81], which contributes to the poor outcomes in the clinical setting ( Figure 3). Here, we summarize the published results in attempt to understand the contribution of CSCs in the clinical setting and postulate how to exploit some unique CSC characteristics to develop novel anti-cancer therapies.

Therapy-resistant CSCs in Disease Relapse
Disease relapse and tumor metastases are some of the major causes behind the unfortunate survival rates in cancer patients who, after a certain point during their course of treatment, fail to respond to conventional therapy. Enhanced therapeutic resistance has been attributed to CSCs [80,[82][83][84][85][86][87], which subsequently leads to increased tumor growth, invasion, and relapse. A common contributor to therapeutic resistance is enhanced DNA damage response. Radiation, in addition to many current chemotherapy drugs (such as Cisplatin, Temozolomide, Methotrexate, and Doxorubicin), induces cell death by disrupting and damaging DNA. Because DNA is the genetic makeup of every cell in the human body, the inability to repair this damage is fatal to the cells [88,89]. The most lethal effect that these previously mentioned forms of therapy have on DNA is produced by double strand breaks. Double strand breaks are typically repaired through either homologous recombination or non-homologous end joining [85,90,91]. For a more detailed summary on homologous and nonhomologous DNA double strand break repair, refer to Cojoc et al. [85].
One particularly interesting DNA repair gene that has been associated with cancer in the context of therapeutic resistance is Rad51 [49,92,93]. During double strand break repair, a template strand will replace the missing base pairs by invading the paired strands of homologous DNA. Rad51 catalyzes the search for and the invasion of the homologous DNA strand as well as the repair initiation and the annealing of the double strand break [94]. Recent data has shown that Rad51 is overexpressed in CSCs and that this increase in expression may be responsible for the therapeutic resistance observed post-radiation and after primary chemotherapy [49,[95][96][97][98]. Furthermore, the inhibition of Rad51 promoted the resensitization of these previously resistant cells to anti-cancer therapy [99][100][101], which suggests that enhanced DNA repair activity in CSCs promotes their ability to overcome any double strand breaks during therapy.

Targeting the CSC Niche for Anti-cancer Therapy
Conventional chemotherapy, which includes any combination consisting of surgery, radiation, and chemotherapy, is currently the main form of treatment for cancer patients. However, there are a number of cancers that quickly become drug resistant and cause disease relapse or tumor metastases. Because CSCs have been shown to have a higher potential to resist conventional therapy and also possesses inherent self-renewal properties, it is crucial to develop strategies that will target these aggressive populations. The intratumoral microenvironment retains the ability to enrich and initiate stemness in cancer cells, so by preventing cellular plasticity through stem niche factors, the CSC phenotype can also be diminished. As previously mentioned, one tumor microenvironmental factor that has been intensively studied is hypoxia. Targeting hypoxia can manipulate the CSC niche, and both HIF1α and HIF2α have been shown to be promising therapeutic targets in glioma [18,102,103]. Anti-angiogenic therapy can also diminish the tumor vasculature as well as inhibit the self-renewing capacities of CSCs [46,104]. Inhibiting angiogenic factors will lead to the starvation of tumors, further inhibiting tumor growth and proliferation of CSCs. However, a pre-clinical study by Schnegg et al. indicated a possible cellular plasticity mediated enrichment of CSCs post anti-VEGF therapy and thus may require further optimization of such therapeutic approaches [73].
Also, to targeting the stem cell niche, increased expression of Rad51 in cells with CSC-like phenotypes has been associated with chemoresistance [49,95]. The enhanced ability of CSCs to repair double-strand breaks in their DNA allows them to overcome damage made postradiation and/or chemotherapy. Recent publications show that inhibiting Rad51 expression, specifically in the CSC subpopulation, causes once-resistant tumors to become re-sensitized to therapy [99][100][101]. There has also been an increase in the search for new drugs that can directly target and kill CSCs by inducing toxicity, inhibiting self-renewal, or sensitizing them to future therapy [64,[105][106][107][108]. However, being able to target CSCs has been an incredibly difficult hurdle to overcome due to the inherent plastic nature of tumor cells to become CSC-like. A promising strategy includes the differentiation of these CSCs to adopt a more benign, lineage-committed fate so they will have a more therapy-sensitive, less aggressive and invasive phenotype. By constantly inducing the differentiation of CSCs, they can be targeted without the challenge of cells reverting to their stem-like state. It is known that bone morphogenetic proteins (BMPs) initiate differentiation of stem cells and can result in decreases in proliferation, tumor growth and initiation [109][110][111][112][113][114][115]. BMP administration has been combined with currently existing chemotherapy [116][117][118][119][120][121], and by doing so, the dynamic conversion of non-CSCs to cells possessing a therapy resistance CSC-like phenotype can be targeted to enhance therapeutic sensitivity as well as efficacy (Table 1).

Conclusion
A recent model of tumorigenesis states that a small subset of cells within the cancer population, known as Cancer Stem Cells, is responsible for the initiation and expansion of tumors. Cells with this stem-like phenotype possess a higher potential for self-renewal and tumor formation and have also been shown to be more resistant to conventional therapies. CSCs exist in a dynamic equilibrium with non-CSCs, and any shift in this balance will potentiate the negative effects of cancer. Although it is known that CSCs can differentiate to have lineage-committed fates, it has now been postulated that these differentiated cells can dedifferentiated and adopt CSC-like phenotypes. Conventional therapies can further enhance the frequency of plasticity as the tumor microenvironment continues to change and pressure the equilibrium to shift, favoring the survival of CSCs. To prevent the aggressive, invasive, and resistant nature of the CSCs, it is crucial to better understand both CSC biology and their niche within tumors. It is not clear if the current stem cell model can account for such reversible intraconvertibility. If the frequency of such conversion is low, it may possible to distinguish the newly converted CSCs and incorporate their biological effects in the current model. However, in light of recent reports that demonstrate that such conversion is significantly accelerated post oncogenic transformation and during anti-cancer therapies, the current CSC model may be unfit for this condition. New models may be required to describe the plastic behavior of human cancer [5,6,10]. Elucidating the mechanisms that drive cellular plasticity and exploring how intratumoral microenvironmental changes affect plastic behavior of cancer cells can further enhance clinical efficacy of current therapies to benefit the many patients and families affected by human malignancies. Hierarchy leading to heterogeneity. The CSC theory claims that CSCs arise from normal stem cells within the population and that only these CSCs drive tumorgenesis. These CSCS can either self renew or divide into differentiated progeny. Since the cells in a tumor come from different CSCs, the tumor is heterogenic. Lee   Asymmetric division of CSCs. (A) Asymmetric cellular division yields one undifferentiated CSC and one differentiated cell. Under normal conditions, this is the second most common division, but during TMZ treatment this type of division occurs least. (B) Symmetric cell renewal produces two daughter CSCs that are identical to the mother CSC. Under normal conditions, this is the least observed division, but becomes the most prevalent when the population is treated with TMZ. This largely contributes to the resistance and reoccurrence of tumors. (C) Symmetric differentiation yields two differentiated progeny. Under normal conditions, this division occurs most frequently. However, during TMZ treatment, this division is seen second most often.  CSC, microenvironment and intratumoral equilibrium. When a tumor is exposed to hypoxia, low pH, chemotherapy or radiation, a microenvironment that favors CSCs is created. Because of this, some mature cells in the tumor dedifferentiate and stemness in the present CSCs is maintained. This plasticity of the tumor leads to drug resistance and disease reoccurrence.  Table 1 Recent findings depict the clinical relevance of CSCs and suggest novel treatment modalities to shrink the CSC population and reduce the inavsive and therapeutic resistant properties of tumors.

Finding Significance Reference
CSCs are senescent Dormancy allows CSCs to resist conventional cancer treatments. [78,79] CSCs overexpress Rad51 Rad51 assists in DNA repair following double strand breaks. Overexpressing Rad51 allows CSCs to repair damage caused by chemotherapy and radiation. [49,[95][96][97][98] BMPs induce differentiation in stem cells Treating CSCs with BMPs may initiate differentiation and thus decrease proliferation and tumor growth.