Ether-Type Moieties in the Lipid Part of Glycoinositolphospholipids of Acanthamoeba rhysodes

Ether lipids were identified among components liberated with HF and nitrous acid deamination from Acanthamoeba rhysodes whole cells and its membrane glycoinositolphospholipids (GIPL). Liberated ether glycerols were converted to various derivatives that served characterization thereof. These included TMS and isopropylidene derivatives, oxidation with sodium periodate to aldehyde followed by reduction with NaBH4 to alcohol, and reaction of the alcohol with acetic anhydrite to form acetate derivatives. Periodate sensitivity demonstrated that the alkyl side chains were linked to the sn-1 position of glycerol. Combined information from TLC, GC–MS analysis, MALDI-TOF spectrometry, and chemical degradation experiments indicated the presence of ether-linked saturated normal and branched hydrocarbons with a length of C20–23 in the phospholipid fraction, C20–24 in free GPI, and C21–23 in the LPG polymer. The distribution of particular classes of alkylglycerols was similar for phospholipid and GPI fractions, and amounted to 2.62 % (±0.04–0.28) 1-O-eicosanyl-sn-glycerol, 16.66 % (±0.32–1.1) 1-O-uncosanyl-sn-glycerol, 9.18 % (±0.33–1.37) anteiso-1-O-docosanyl-sn-glycerol, 47.56 % (±0.32–2.14) 1-O-docosanyl-sn-glycerol, 20.56 % (±0.58–1.67) anteiso-1-O-tricosanyl-sn-glycerol, and 2.34 % (±0.12–0.63) 1-O-tricosanyl-sn-glycerol. For LPG preparation, the most abundant were anteiso-1-O-tricosanyl-sn-glycerol (57.26 %) and 1-O-docosanyl-sn-glycerol (30.12 %). The data from TLC and GC–MS analysis showed that ether lipids from phospholipids probably represent the lyso-alkylglycerol type, while those derived from GIPL are alkylacylglycerol moieties.


TLC Thin layer chromatography TMS Trimethylsilyl group
Introduction 1-O-Alkyl-sn-glycerols usually form the backbone of complex ether-linked glycerolipids, including biologically active lipids e.g. PAF (platelet-activating factor) and plasmalogens [1]. They were also identified in parasite Leishmania lipophosphoglycan and glycoinositol phospholipids as 1-alkyl-2-acylglycerol and lyso-alkyl phosphatidylinositol backbones. These ether-type lipid moieties were unusual since they contained predominantly 18:0, 22:0, 24:0, or 26:0 alkyl chains. An increase in the content of 24:0 and 26:0 alkyls was observed with elongation of the carbohydrate chain and appeared almost exclusively in lipophosphoglycan [2,3]. The existence of long alkyl chains in ether lipids is a rare feature. In general, alkyl chains contain an even number of carbons ranging from C14 to C22 (both saturated or monounsaturated), and saturated or monounsaturated O-alkyl chains of 16 and 18 carbon atoms are the most prevalent. Odd-numbered polyunsaturated and branched chains are only minor components [1]. It has been demonstrated that synthetic alkylacylglycerols corresponding to lipid parts of Leishmania donovani GPI [4], those derived from products of Leishmania lipophosphoglycan hydrolysis as 1-O-alkyl-2-lyso-glycerol [5] as well as the major surface molecules (glycoinositolphospholipids and lipophosphoglycan) isolated from the parasite cells and bearing ether lipids, are antagonists of protein kinase C (PKC) in vitro [4,5]. Taken together, it was proposed that ether lipid moieties and not the carbohydrate domain could modulate the signalling pathways. Short-chain alkylglycerols have also been shown to increase permeability of tight junctions in the blood-brain barrier (BBB) [6] and facilitate transport of some drugs [7]. Successful treatment of many brain disorders seems to be impossible because of very limited penetrations of drugs across the BBB, but the demonstrated property of alkylglycerols suggests that they possess several potent pharmacological activities.
Glycoinositolphospholipids (GIPL) are heteropolymers in which the sugar portion is coupled to the lipid moiety via an intermediate inositol phosphate. In turn, glycosylphosphatidylinositol (GPI) anchors are a class of GIPL which carry a conservative core structure ''Mana1-2Mana1-6Mana1-4GlcNH 2 a1-6myo-inositol-1-PO 4 -lipid''. The lipid moiety in the GPI structure can vary in the nature and the core glycan can have side-chain modifications such as ethanolamine phosphate, mannose, galactose, or sialic acid [8]. The chemical composition of the aliphatic residues is dependent on the organism and the stage in its life cycle. In a majority of eukaryotes, GPI are responsible for binding with a wide group of plasma membrane proteins. In protozoan organisms, they exist in the membrane as free or carry a carbohydrate moiety, e.g. in the lipophosphoglycan of Leishmania and lipophosphonoglycan (LPG) of Acanthamoeba. In Leishmania they are based on a type-2 GPI core, Mana1-3Mana1-4GlcNH 2 a1-6 PtdIns [9].
Acanthamoeba spp. belongs to parasitic protozoa responsible mainly for granulomatous amoebic encephalitis (GAE) and acanthamoeba keratitis (AK). The lipid content and isolation, purification, and partial characterization of LPG from plasma membranes of Acanthamoeba species have been described previously. Basic chemical analyses have contributed to establishing the composition of 77 % of LPG by weight containing: 26 % neutral sugars (Glc, Man, Gal, Xyl), 3.3 % amino sugars (GalN, GlcN), 8 % inositol, 10 % aminophosphonates (AEP, 1-OH AEP), 3.2 % acid-hydrolyzable phosphate, 14 % fatty acids, and 13 % long chain fatty acids [10][11][12]. In those reports, the described lipid backbone of LPG was the ceramide-type. In the present study, we pre-analyzed lipids liberated with HF from whole cells of Acanthamoeba rhysodes but focused on these derived from isolated GIPL (LPG and free GPI). Among lipids released from whole cells, we identified lysoalkylglycerols with saturated normal and branched hydrocarbons with a length of C 20-23 as the side chain, which probably came from phospholipids. In the GPI fraction, alkyls with a length of C 21-24 occurred, and the distribution of the particular classes was similar to that obtained from phospholipids. In contrast, only C 21-23 AKG were identified in the LPG preparation. The most abundant among the ether lipids discovered in A. rhysodes were 1-O-docosanylsn-glycerol in phospholipid and GPI fractions and anteiso-1-O-tricosanyl-sn-glycerol in LPG, respectively. To our knowledge, this is the first report of this kind of ether lipids in Acanthamoeba.

Microorganisms and Culture Conditions
Acanthamoeba rhysodes (Hartmannella rhysodes) Changstrain was obtained from the culture collection of Poznań University, Poland. The endocytobiont free amoebae were grown axenically in 300-mL Erlenmeyer flasks containing 100 mL of PYG (peptone yeast glucose) medium, pH 6.6. The chemical composition of the PYG medium was essentially the same as that described by Band [13] and consisted of 15 g proteose peptone 3 (Difco), 5 g yeast extract (Difco), 10 g glucose, 120 mg NaCl, 3 mg MgCl 2 6H 2 O, 3 mg CaCl 2 , 3 mg FeSO 4 , 142 mg Na 2 HPO 4 , and 136 mg KH 2 PO 4 in 1 L. The culture was incubated on a rotary shaker with an acentric rotation of 3 cm (120 rev/ min) at 28°C. Amoebae from the early stationary phase of growth were harvested by repeated centrifugation at 3009g for 10 min with washing 0.15 M KCl. Samples of whole cells submitted to hydrolysis (4 M HCl/100°C/4 h) and n-hexane soluble products as TMS derivatives were analyzed in GC-MS.
Extraction and Purification of Glycoinositolphospholipids Glycoinositolphospholipids were purified as described earlier [14]. The saline-washed cells were suspended in two volumes of fresh 0.15 M KCl, and disrupted in a Potter disintegrator in ice. The homogenate was ultracentrifuged for 3 h at 123,500g (Beckman rotor Type 50.2 Ti) [11]. The sediment (a crude membrane pellet) was treated with phenol-water [15] at 65°C three times. The combined aqueous phases of the hot phenol-water extraction were dialyzed against tap water and lyophilized. The deposited material was then extracted with 20 volumes of mixture chloroform/MeOH (2:1, by vol.) according to the method of Korn et al. [11] to remove traces of phospholipids, washed with acetone, washed twice with water, and lyophilized. The delipidated material was extracted three times with 9 % n-butanol for 3 h, centrifuged, and the supernatants obtained were combined and rotary evaporated. Then, the GIPL obtained were subjected to n-butanol/water (1:1, by vol.) partition [16]. The water phase was washed twice with a fresh portion of butanol. During that process, LPG (the aqueous phase) was separated from residual free GPI (butanol phase) (Scheme 1). Both phases were dried and analyzed. The efficiency of the purification protocol for LPG was assessed by SDS-polyacrylamide gel electrophoresis and GC-MS analysis of fatty acids [14].
Releasing the Glycerol Lipids with HF Briefly, (1) 30 mg of dry cells, (2) 20 mg of GIPL were treated with 1 mL (250 lL for GIPL) of ice-cold HF for 48 h at 0-4°C with mixing. The hydrofluoric acid was removed by nitrogen flushing. The pellets obtained were subjected to extractions: chloroform/water (1:1 by vol.; 1 mL each) for lipids liberated from whole amoeba cells, and with subsequent organic solvents: chloroform, hexane, diethyl ether (1 mL each), followed by partition with the water/butanol system 1:1 by vol.; 1 mL each for lipids liberated from GIPL. Organic phases for particular samples were combined and dried with a flush of nitrogen and subjected for further analyses.

Characterization of Ether Lipids Liberated with HF
Conversion of small portions of lipids liberated with HF from whole cells and separated in TLC using the solvent system chloroform/MeOH (1:1, by vol., solvent 1) [12] was conducted to obtain (A) TMS and (B) isopropylidene derivatives [17,18]. To obtain the isopropylidene derivative, the sample of lipids was dissolved in 1 mL of acetone, followed by addition of 5 lL of concentrated HClO 4 , and incubated at room temperature for 15 min. Then, 1 M ammonium hydroxide (40 lL) was added for neutralization. The sample was brought to dryness, which was followed by partitioning with 2 mL each of chloroform and water. The chloroformsoluble products were washed twice with water, dried over sodium sulfate, and concentrated under a flux of nitrogen. The authentic standards (both 25 lg): butyl alcohol (Aldrich) and N-tetracosanoyl-phytosphingosine (Matreya) were also converted to an isopropylidene derivative. As isopropylidene derivatives, the sample and standards were subjected to TLC in the solvent system hexane/diethyl ether/ acetic acid, 80:20:1, by vol., (solvent 2) [19], and GC-MS (except the ceramide derivative). Additionally, the TMS derivatives were analyzed by GC-MS.
The samples of HF-released lipids were subjected to solvolysis with 3 M HCl in MeOH for 18 h at 80°C and 1 M KOH in MeOH for 18 h at 100°C [17]. The products were partitioned with chloroform/water in a final ratio of 1:1. Organic solvent extracts were dried under a stream of nitrogen and the lipophilic products obtained were converted to TMS derivatives, which was followed by GC-MS analyses. For ether cleavage, the sample of ether lipids liberated with HF was treated with HI according to the Orlandi and Turco [17] method. An aliquot was dissolved in 1 mL of hydroiodic acid (57 %) and treated for 18 h at 120°C under a nitrogen atmosphere. Then, it was extracted three times with 2 mL of diethyl ether. The combined ether extracts were washed successively with water and saturated potassium bicarbonate to remove excess hydroiodic acid. Free iodine was removed by extraction with a 50 % solution of sodium thiosulfate. The ether extract (of alkyl iodides) was cleaned in column chromatography on silica gel with n-hexane elution [20], then dried, dissolved in chloroform, and analyzed by GC-MS.
HF released lipids were also treated with sodium periodate followed by reduction and acetylation according to Orlandi and Turco [17]. The sample (30 lg) was dissolved in 0.5 mL of a chloroform/MeOH/water (16:16:5, by vol.) mixture adjusted to pH 9.5 with NH 4 OH, to which 0.5 mL of 0.057 M sodium periodate was added in the same solution. After incubation in the dark at room temperature for 16 h, 1 mL of MeOH/water (1:1, by vol.) and 1.5 mL of chloroform were added to the reaction mixture, vortexed, and centrifuged. The organic phase was taken to dryness under a stream of nitrogen, redissolved in 0.5 mL of MeOH, and reduced with 0.5 mL of 2.64 M NaBH 4 in 0.04 M NaOH for 1 h at 45°C. The reaction mixture was then partitioned after addition of 0.03 mL of 1 M HCl, 0.52 mL of H 2 O, 0.3 mL of MeOH, and 1.6 mL of chloroform. The organic solvent phase was washed twice with 0.5 mL H 2 O, taken to dryness under a stream of nitrogen, and acetylated with acetic anhydrite/pyridine (1:1, by vol.) for 0.5 h at 100°C. After suspension in n-hexane, the alkylglycerol acetates obtained were analyzed by GC-MS. The same treatment was applied to 25 lg of the authentic standard of butyl alcohol (Aldrich).

Analysis of Ether Lipids Liberated from of Glycoinositolphospholipids
Samples of (1) GIPL, (2) purified LPG, and (3) GIP were hydrolyzed according to the method of Watanabe et al. [21]. Briefly, 5 mg of the appropriate sample was treated with 1 mL of the MeOH/water/HCl mixture (11:2.6:1, by vol.) at 80°C overnight, and the fatty acid methyl esters (FAME) combined with ether lipids were extracted into n-hexane, and derivatized with TMS reagent prior to GC-MS analysis. 25 lg of the authentic standard of butyl alcohol was converted to TMS derivatives as well and analyzed in GC-MS.
Lipids liberated from GIPL with HF subjected to: (A) conversion to isopropylidene derivatives and TLC in solvent 2, (B) acid hydrolysis followed by conversion to isopropylidene and TLC chromatography in solvent 2, (C) conversion to TMS derivatives and GC-MS analysis, (D) acid hydrolysis, hexane/water extraction, and conversion of organic soluble products to TMS derivatives and GC-MS analysis.
The sample of LPG was subjected to deamination according to Caroff et al. [22] with a minor modification. Briefly, about 25 mg of the preparation was suspended in 1.25 mL of water and equal volumes of 33 % acetic acid and 5 % sodium nitrite. After 2.5 h of incubation at 37°C, a fresh portion (0.2 mL) of acetic acid and sodium nitrite was added, and the incubation continued for another hour. The lipids liberated during deamination were extracted with n-butanol (7 mL). The aqueous phase was rinsed twice with organic solvent and combined butanol fractions were dried under reduced pressure. After desalting on Dowex-50 W H ? , the water-soluble deaminated products were freeze-dried, reduced (NaBH 4 ), desalted again on a Dowex-50 W H ? column, and co-distilled with MeOH. Aliquots of the material were subjected to hydrolysis (2 M TFA/120°C/2 h), reduction, and peracetylation. The butanolic fraction of lipids liberated with deamination was submitted to acid hydrolysis (4 M HCl/100°C/4 h) followed by extraction with n-hexane, and conversion to TMS derivatives. The aqueous acid hydrolysate was dried and converted to an O-trimethylsilyl derivative with BSTFA/ pyridine (1:1, by vol.) for an hour at 20-22°C. All the samples were analyzed by GC-MS.

Analytical Methods
TLC was performed using Silica gel 60 F 254 (Merck) plates with the solvents 1 and 2. Chromatograms were visualized with iodine vapour for the tested samples and with 10 % H 2 SO 4 in 70 % methanol for the authentic standards. The initial concentration of the samples and standards were 25 mg/mL. Individual lipid classes liberated with HF were separated by preparative TLC using solvent 1. The bands obtained were scraped and eluted from the silicic acid with chloroform/MeOH/water (25:15:2.5, by vol.), filtered through Whatman No. 1, suspended in chloroform, and kept in 4°C for further analysis [12]. Aliquots from separated fractions (samples A-D) were subjected for further analyses by GC-MS. Sample D was also analyzed by MALDI-TOFMS. Isopropylidene derivatives were separated in TLC with solvent 2 and eluted from silicic acid with the same mixture prior to analysis.
The LPG preparation (5 lg) was separated in 12.5 % SDS-tricine polyacrylamide electrophoresis gel [23] and the bands were visualized by silver staining after oxidation with periodate according to the method of Tsai and Frasch [24].
MALDI-TOF was performed with the Zenker et al. [25] method using acetonitrile as a solvent to obtain a 50 % (v/ v) 2,5-dihydrobenzoic acid (DHB) matrix on a Voyager-Elite (PE Biosystems) instrument fitted with a VSL-337 ND nitrogen laser (337 nm) and operated in the linear mode, at an accelerating potential of 20 kV, with positive or negative detection. The spectra obtained were the averages of 150 scans. Then, 12.5 lg of the sample (from spot D) was mixed with an equal volume of the DHB matrix solution, and spotted and dried on a MALDI-TOF sample plate.
The TMS and isopropylidene alkylglycerol derivatives, alkyl iodides, and alkylacetylglycols derived during periodate oxidation were analyzed by GC-MS in the EI mode. The analysis was performed on an Agilent 7890A-5975C instrument equipped with a capillary column (HP-5MS, 30 m 9 0.25 mm), applying a temperature gradient of 150°C (5 min) to 310°C at 5°C min -1 .

Analysis of Acid Hydrolysis Products from Whole Amoeba Cells: Ether Lipids
Among the acid hydrolysis products liberated from whole amoeba cells and extracted with hexane, ether lipids were also identified by GC-MS. The analysis of the chromatographic data indicated the presence of seven species of alkylglycerols with ether-linked saturated normal and branched hydrocarbons with a length of C 20-23 (n20:  Table 1). No peak for 2-O-alkyl-glycerol was identified. Assignment of the monoalkylglycerols followed the MS fragmentation patterns published by Bertello et al. [27], Myher et al. [28], and Orlandi and Turco [17].

Analysis of Products Liberated with HF from Whole Amoeba Cells
The residues liberated with HF from whole amoeba cells were submitted to chloroform:water extraction. Organic solvent-soluble products were separated by TLC carried out on silica gel plates, and developed with solvent 1 [12]. Four bands (A, B, C, D) with an R F of 0.0, 0.21, 0.37, and 0.85 respectively, were detected by iodine vapor (Fig. 1). The material from appropriate bands was scraped and eluted from silicic acid, and subjected to GC-MS analysis after conversion into TMS derivatives. The ether lipids were identified only in the material derived from spot D with R F 0.85 and their chromatographic profile was almost identical to that obtained for the material subjected to acid hydrolysis. The only minor difference was the lack of the peak for branched 1-O-heneicosyl-sn-glycerol comprising around 1 % of the whole ether lipids pool. The chromatographic analysis also indicated that, beside ether lipids, the examined material from spot D contained monoacylglycerols and free fatty acids (16:0, 18:0, 18:1), which co-migrated with them on the TLC plate. It is known that, in terms of chromatography, solubility, and certain other physical properties, glyceryl ethers behave in exactly the same way as acylglycerols [19].
The present study confirms the prediction of Johnson and Holman [30] that unsaturated monoacylglycerols show the highest parent ion intensities. Unsaturated ethers containing double bond(s) in the chain also exhibit a more pronounced molecular ion, which was not detected for the saturated ones. Also other ions typical for TMS ethers of 1-monoacylglycerols (55, 57, 67, 69, 73, 103, 129, 147 To separate the constituents of spot D, they were converted to 1-radyl-2,3-O-isopropylidene derivatives. The 1-alkyl-2,3-O-isopropylidene derivatives were separated from 1-acyl-2,3-O-isopropylidene by TLC using solvent system B. Three spots with an R F 0.27, 0.42, and 0.59 were visualized with iodine vapour (Fig. 2a). The individual spots were scraped and directly analyzed by GC-MS. The base peak in the spectra of the isopropylidene derivatives of lyso-glyceryl ethers at 101 m/z [31] (Fig. 2b)    To confirm that alkylglycerols identified on the basis of GC-MS structural analysis (Fig. 3a) belong to ether-type lipids, they were subjected to alkaline methanolysis and acid methanolysis. In both conditions of the degradation procedures, some HF-released lipids were insensitive to solvolysis (Fig. 3b, c). Based on these results, it can be deduced that they do not belong to the sphingosine-, plasmalogen-, or acylglycerol-type of lipids because they are not stable under such treatment [17]. The only class of lipids exhibiting stability under such hydrolytic conditions is glycerol ethers in which alkyl side chains are linked to the glycerol backbone through stable ether linkages. The peaks marked as 7, 11, 12, 13, 14, and 15 corresponding to alkylgycerols, presented in Table 1, were observed in all ionograms. However, some peaks (1, 3, 6) corresponding to TMS ethers of saturated monoacylglycerols (2,3-dihydroxypropyl myristate, 2,3-dixydroxypropyl palmitate, 2,3dixydroxypropyl stearate) were still identified after alkaline methanolysis (Fig. 3c). The monoglyceride content among the alkaline solvolysis products was probably caused by equilibrium reactions of transesterification [32][33][34], since peaks corresponding exclusively to monoalkylglycerols were observed after acid methanolysis (Fig. 3b). Besides, peaks corresponding to methyl esters of fatty acids (saturated C14-19:0, and unsaturated 16:1, 18:1, 20:2, 20:3, 20:4, 30:2, 30:3) appeared in the chromatogram of methanolysis products. This kind of fatty acids were identified previously [35] in the acylglycerol-type of lipids in Acanthamoeba castellanii, which might confirm that they originated from monoacylglycerols in our sample. This result was well correlated with disappearance of all peaks corresponding to monacylglycerols (both 1-and 2-isomers).

Ether Cleavage
The presence of ether-bound alkyl side chains in the HFliberated lipids (spot D) was confirmed by cleavage thereof with hydroiodic acid [17]. The resulting alkyl iodides were directly analyzed by GC-MS. Although it is known that GC-MS analysis operating in the EI mode shows limits of detection of this kind of compound [36] by the lack of molecular ions, the loss of iodide [M ? H -I] ? as the ions at 282, 296, 310, 324 m/z for alkyl chains with particular lengths were observed in the spectra of the derivative products (Fig. 4). This kind of fragmentation process is the most important for the heavier alkyl halides (including iodine) and involves simply losing the halogen to form an alkyl carbocation (ipso-cleavage) [37]. According to the spectra of alkyl iodides, eicosyl-, heneicosyl-, docosyl-and tricosyl iodides, respectively, were identified. The HF treatment of whole A. rhysodes cells liberated seven classes of monoalkylglycerols (Table 1). To determine definitively whether the alkyl substituents in ether lipids were at the C2 or C3 position of the glycerol backbone, lipids from spot D were treated with periodate, followed by reduction and acetylation to obtain appropriate radyl-glycol acetates. The resulting products were analyzed by GC-MS. The chromatography profile analysis of glycols (Fig. 5a) and TMS derivatives of AKG (Fig. 3b) showed close similarities, except for the retention times for the individual particles changed by the mass reduction in the case of glycerol residue subjected to oxidative cleavage. No molecular ions of radyl-glycols were formed in the EI mode, but ions with loss of ketene [M-42] ? were identified for almost all glycols derived from normal and branched 1-O-alkylglycerols treated with periodate (Fig. 5b). Exceptionally, no peak for normal and branched glycols derived from tricosanyl glycerols were observed in the chromatogram of ether lipids. This may have been caused by the small amount of the sample, higher masses of the derivative products, and poor efficiency of oxidation.
Since all the monoalkylglycerols showed similar spectra as the TMS ethers with the characteristics of the 1,2-diol ion at m/z 205, it can be concluded that tricosanyl glycerol had a side chain in the sn-1 position as well. Therefore, the spectra obtained confirmed that all the alkyl moieties were substituted at the sn-1 position of the glycerol backbone.

Isolation of Glycoinositolphospholipids (GIPL) from Amoeba Membrane
The crude membrane obtained according to the method of Korn et al. [11] was subjected to extraction with hot phenol. The material from the aqueous phase [15] was delipidated with a chloroform-methanol solvent and washed with acetone [11]. All the procedures were applied to remove proteins and neutral and polar lipids from A. rhysodes plasma membrane. Subsequently, extraction with 9 % n-butanol was applied to obtain glycoinositolphospholipids and partition with the n-butanol-water mixture to separate LPG (water fraction) from residual free GPI (butanol fraction) [16] (Scheme 1). When analyzed by SDS polyacrylamide gel electrophoresis, the LPG obtained during the purification steps yielded two silver stained bands of high electrophoretic mobility, typical for this polymer. The chromatographic analysis of the fatty acids released by acid hydrolysis from LPG showed exclusively long chain saturated normal and branched FA (C [16][17][18][19][20][21][22][23][24][25][26][27][28] ) and normal and branched a-hydroxy (C 20-28 ) characteristic for LPG of Acanthamoeba [14].
The GIPL sample was exposed to dephosphorylation with HF to liberate the lipid part. Subsequently, exhaustive extraction of the liberated products was carried out with different solvents. Lipid moieties obtained from GIPL were converted to isopropylidene derivatives and separated in TLC using solvent system B. Two spots were observed on the plate with R F 0.59 and 0.27 corresponding to fatty acids and MAG, respectively. Additionally, no spot with R F 0.42 corresponding to monoalkylglycerols was revealed (Fig. 7). The spot for AKG, except for FA and MAG, appeared when the products liberated by HF from GIPL were subjected to acid hydrolysis prior to conversion to isopropylidene derivatives and TLC (Fig. 7). The same results were obtained when lipids liberated from GIPL through dephosphorylation were silylated and directly analyzed in GC-MS. No peak related to the TMS derivative of AKG was observed in the chromatogram. Besides, peaks corresponding to AKG were recorded when the same material was hydrolyzed and treated with TMS. This may suggest that ether lipids in the lipid part of GIPL are alkylacylglycerols, but not the lyso-alkyl type.
To confirm that the AKG identified are the lipid part of LPG, the sample of LPG was subjected to nitric acid deamination. Among the products liberated during the process, four classes of C 21-23 AKG with a chromatographic profile similar to that obtained after acid methanolysis (Fig. 6b) and phytosphingosines t25:0-27:0 (normal and branched) reported earlier as being a part of the ceramide moiety of LPG were identified [14]. Additionally, butanol soluble products liberated through deamination contained inositol, whereas 2,5-anhydromannitol derived from unsubstituted glucosamine was identified among the water-soluble ones [38]. These results show that the ether glycerol lipid moiety is bound with GlcN through phosphoinositol, which proves that it is a part of the LPG polymer.

Discussion
Glycerol based ether lipids are normally minor constituents of most cell membranes in mammals; in contrast, they are major constituents in cell membranes of archaean [39] and some protozoan parasites [16]. The most prevalent ether backbones found in nature consist of 1-O-alkyl or 1-O-alk-1 0 -enyl. Until recently, they have been considered to be little more than a biological novelty. However, findings of elevated levels of ether lipids in cancer tissues, followed by the discovery of distinctive ether lipids, such as plateletactivating factor (PAF), with important biological activities have greatly stimulated the interest in these compounds. Both synthetic and naturally occurring alkylglycerols themself possess several potent pharmacological activities.
In vitroand in vivo studies of ether lipids have shown that they mediate immune response. 1-O-Dodecylglycerol in trace amounts (0.05 lg/mL) was able to induce markedly elevated ingestion activity of macrophages. In turn, higher amounts thereof were toxic. It is a potent gram-positive antibacterial agent effectively acting at a relatively low concentration (4 lg/mL) against Streptococcus faecium ATCC 9790 through stimulation of peptidoglycan hydrolase (autolysin) as well [1]. A synergistic effect of 1-O-dodecylglycerol with penicillin G was shown in experiments with the same and other species of bacteria [40]. A similar event was observed with amphotericin B (antifungal agents) [41]. As a part of diacyl glyceryl ethers, AKG was also shown to strongly inhibit the growth of three prostate cancer cells (Du-145, PC-3, PCa-2b) [1]. On the other hand, ether lipid levels in other types of cancer cells (e.g. breast cancer) are elevated. Their specific function remains unclear but inactivation of the alkylglyceronephosphate synthase (a key enzyme in the biosynthesis of glycerol-ether lipids) led to significant impairments in cancer pathogenicity and its overexpression increased cancer cell motility, survival, and tumor growth [42]. Ether lipids are highly abundant in nervous tissues and play a major role as cellular antioxidants. Changed levels are associated with neurological dysfunctions and degeneration [43]. Other functions of ether lipids are not well understood, but they have been implicated in maintaining physiochemical properties of cell membranes, such as fluidity, fusion events, and lipid raft domains. More examples of the action of glyceryl-ether lipids (such as reduction of injuries, increasing cell permeability, BBB opening) can be found in the very comprehensive review by Iannitti and Palmieri [44]. Ether linked hydrocarbons in parasitic protozoa membranes can occur as constituents of phospholipids and GPI anchored glycoconjugates and glycoproteins. As such, they are involved in many aspects of host-parasite interactions, such as adhesion and invasion of host cells, modulation and evasion from host immune response, and pathogenesis [16].
Cosmopolitan amoebae belonging to the genus Acanthamoeba are the causative agents of some diseases (AK, GAE). Some strains, both virulent and avirulent, can also come into close interactions with pathogenic bacteria, viruses, yeasts, or protists. It is known that amoeba cells are covered with a GPI-anchored polysaccharide called LPG. In the past, 77 % of its constituents were identified but the rest stayed unexplored [12]. It was also established that LPG has a polymorphic nature since two types of LPG differing in the length and composition of oligosaccharide attached to the lipid portion were identified. The lipid part of LPG was established to be a ceramide type with phytosphingosine moieties t24-28:0 substituted with normal and 2-hydroxy long chain fatty acids [12,14]. Current results presented in this report have demonstrated that ether-type alkylacylglycerol moieties exist in LPG as well. They were established to be C [21][22][23] in length and the most abundant were those substituted with the anteiso-C 23 (57.26 %) and nC 22 (30.12 %) alkyl chain. Since electrophoretic analysis of LPG still showed only two bands, with migration presented earlier as typical for that kind of a polymer [12], it seems very plausible that different types of lipid moieties are attached to the same oligosaccharide parts. Lederkremer et al. [45] demonstrated that the GIPL lipid domain structure, in epimastigotes of T. cruzi strain CL collected at the exponential phase of growth, is developmentally regulated, and alkylacylglycerol (1-Ohexadecyl-2-O-palmitoylglycerol) or ceramide (sphinganine-containing) were found linked to the same glycan. In turn, Saccharomyces cerevisiae glycoproteins are anchored by either a glycerolipid or a C 18 -phytosphingosine-containing ceramide. A suggestion has been made that the base sensitive lipid moiety is exchanged by ceramide during glycoprotein maturation [46].
The chromatographic profile of AKG derived from free GPI of A. rhysodes showed seven peaks identified as monoalkylglycerols with attached hydrocarbons with a length of C 20-24 (Fig. 6c) and the predominant was that with an attached C 22 hydrocarbon (47.56 %). They were also identified as AAG moieties. The distribution of particular ether lipids for LPG and GPI was distinct. In LPG preparations, the most abundant AKG were those with the longest branched hydrocarbon chain (C 23 ), while in GPI those with a shorter normal one (C 22 ). Similar results were obtained for Leishmania major glycolipids which generally contained C 18 , C 22 , C 24 , and C 26 alkyl chains, although there was an increase in the proportion of C 24 and C 26 hydrocarbons with elongation of the carbohydrate chain [3]. In Acanthamoeba, the LPG polymer carries longer than GPI oligosaccharide moieties; therefore, the same rule as in Leishmania may apply. GPI moieties in protozoa cells can exist as free structures in the membrane or serve as protein anchors [45,47]. An important feature of the free GPI/ GIPL structures developed by Leishmania and Trypanosoma cruzi, in contrast to those of Plasmodium falciparum and Trypanosoma brucei, is the preponderance of alkylacylglycerols (or lyso-alkylglycerols) and ceramides, as opposed to diacylglycerols, within the lipid domain [47]. The differences in the chemical structure have their implications in the possible function. In preliminary studies, it was established that GPI/GIPL containing a ceramide or a saturated lipid chain in the alkylacyl-PtdIns and lysoalkyl-PtdIns moieties severely inhibit macrophages, while that derived from P. falciparum and T. brucei are potent macrophage activating molecules [4,5,47,48]. The b Fig. 6 Ionograms for the ion at m/z 205 of TMS ethers of monoalkylglycerols liberated in acid methanolysis according to Watanabe et al. [21]. a From GIPL, b LPG, c GPI of A. rhysodes. The number of marked peaks denotes TMS derivatives of ether lipids: 1 C 20 ; 2, 3 C 21 ; 4, 5 C 22 ; 6, 7 C 23 ; 8 C 24 Fig. 7 TLC analysis of lipids liberated from GIPL: left panel. Line 1 With HF; line 2 After methanolysis according to Watanabe et al. [21]. Lipids separated as isopropylidene derivatives in solvent 2 and visualized with iodine vapour. Right panel. Isopropylidene derivative of authentic standard phytoceramide-t18:0(24:0) visualized with sulfuric acid. FFA Free fatty acids, AKG monoalkylglycerols, MAG monoacylglycerols, CER phytoceramides mechanism of mediating immune responses can rely on inhibition of PKC action through ether lipids or ceramide moieties in contrast to the lack of an effect served by dialkyl glycerols [5,49,50]. Thus, LPG of Acanthamoeba containing both the ceramide and alkylacylglycerol type of the lipid portion may be able to subvert the immune system and control the infection of the brain. When whole cells of A. rhysodes were subjected to dephosphorylation with HF, seven classes of lyso-alkylglycerols (C 20-23 ) were released. Since, parasitic protozoa possess, among others, ether-linked hydrocarbons as components of phospholipids [51], and because during our investigations ether lipids were liberated from GIPL of A. rhysodes established exclusively as the alkylacylglycerol type, we deduced that monoalkylglycerols originated from the phospholipid fraction. Hydrolizates of the phospholipid fraction (data not presented) showed a similar chromatographic profile as the dephosphorylated material. However, exact determination of ether phospholipids will be the subject of our future studies. It is also known that parasitic protozoa secrete exosomes, which are proposed to act as messengers to prime host-cells preparing the host for the incoming parasite. Vesicles may arise from plasma membrane budding, which liberates membrane fragments that ultimately form vesicles by fusion of their extremities [52]. The mammalian brain enzymes selective for the plasmanyl and plasmenyl type of phospholipids can be engaged in that process because they participate in creating another type of membrane vesicle-synaptosomes [53]. Taken together with the findings that microvesicles are shed by T. cruzi and engulfed by the host cells to prepare them for the incoming trypanosome, it suggests a potential role of the phospholipid-containing ether moiety from Acanthamoeba in preparing endothelial cells in the BBB to traversing by amoebae. These all are only hypothetical functions of ether lipids that need to be confirmed in future studies.
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