Induced pluripotent stem cell intervention rescues ventricular wall motion disparity, achieving biological cardiac resynchronization post-infarction

Dyssynchronous myocardial motion aggravates cardiac pump function. Cardiac resynchronization using pacing devices is a standard-of-care in the management of heart failure. Post-infarction, however, scar tissue formation impedes the efficacy of device-based therapy. The present study tests a regenerative approach aimed at targeting the origin of abnormal motion to prevent dyssynchronous organ failure. Induced pluripotent stem (iPS) cells harbour a reparative potential, and were here bioengineered from somatic fibroblasts reprogrammed with the stemness factors OCT3/4, SOX2, KLF4, and c-MYC. In a murine infarction model, within 30 min of coronary ligation, iPS cells were delivered to mapped infarcted areas. Focal deformation and dysfunction underlying progressive heart failure was resolved prospectively using speckle-tracking imaging. Tracked at high temporal and spatial resolution, regional iPS cell transplantation restored, within 10 days post-infarction, the contractility of targeted infarcted foci and nullified conduction delay in adjacent non-infarcted regions. Local iPS cell therapy, but not delivery of parental fibroblasts or vehicle, prevented or normalized abnormal strain patterns correcting the decrease in peak strain, disparity of time-to-peak strain, and pathological systolic stretch. Focal benefit of iPS cell intervention translated into improved left ventricular conduction and contractility, reduced scar, and reversal of structural remodelling, protecting from organ decompensation. Thus, in ischaemic cardiomyopathy, targeted iPS cell transplantation synchronized failing ventricles, offering a regenerative strategy to achieve biological resynchronization.


Introduction
Cardiac pump function relies on coordinated myocardial motion secured through ordered electromechanical activation (Bers & Harris, 2011). Development of cardiac dyssynchrony accelerates decompensation of heart function, and is commonly associated with progressive organ failure (Kass, 2009). In the setting of myocardial infarction, the discrepancy in myocardial viability between infarcted and non-infarcted areas generates an environment conducive to electrical and mechanical dyssynchrony (Nucifora et al. 2010). Florid dyssynchrony has a detrimental impact on ventricular ejection volume, diastolic filling and valve function, precipitating pump failure and leading to poor outcome (Shin et al. 2010). Introduction of cardiac resynchronization therapy (CRT) has recently offered a major advance in managing end-stage cardiomyopathic disease. Device-based CRT corrects conduction delays, yet fails to address the origin of contractile deficit (Auricchio & Prinzen, 2011). As a result, the non-viable myocardium remains insufficiently resynchronized by pacing, and dyssynchrony continues uncorrected (Daubert et al. 2012). Indeed, a third of patients that received CRT regimens have not responded optimally (Abraham et al. 2009;Adelstein et al. 2011). Strategies that would afford tissue repair and ensure synchronization of dysfunctional myocardium are thus warranted.
Regenerative interventions are increasingly considered in the management of ischaemic cardiomyopathy (Bartunek et al. 2010;Wollert & Drexler, 2010;Penn et al. 2011). Multiple candidate cell types have been isolated from cardiac and non-cardiac sources (Janssens, 2010). In this regard, nuclear reprogramming provides an advanced platform to reset cell fate and bioengineer pluripotent stem cells from somatic tissue sources (Yamanaka, 2012). Derived induced pluripotent stem (iPS) cells harbour the potential to form functional cardiac tissue, and to reconstruct heart muscle (Nelson et al. 2010;Mauritz et al. 2011). To date, however, the impact of iPS cell therapy on cardiac dyssynchrony has not been tested. In the present proof-of-concept study, performed using a murine infarction model, targeted iPS cell transplantation into infarcted myocardial regions restored local wall motion and prevented chronic remodelling achieving cardiac resynchronization.

Ethical considerations
All protocols were carried out under the National Institutes of Health guidelines with approval obtained from the Institutional Animal Care and Use Committee, and the Biosafety Committee at Mayo Clinic. All procedures on living animals were conducted under general inhalation anaesthesia. Animals demonstrating signs of organ failure were removed from the study for humane considerations and sacrificed with carbon dioxide. Following recommendations of the American Veterinary Medical Association and the Institutional Animal Care and Use Committee, all animals were sacrificed with carbon dioxide at the end of the study.

Bioengineered pluripotent stem cells
Fibroblasts, i.e. mouse embryonic fibroblasts from a DR-4 strain which is a mixed background of 129/Sv, BALB/c and C57BL/6 strains, served as the somatic tissue source and were transduced with human cDNA encoding the reprogramming factors OCT3/4, SOX2, KLF4 and c-MYC packaged in a lentivirus (Fig. 1A-D;Nelson et al. 2009). Cells were labelled with HIV vectors carrying LacZ (pLenti6/UbC/V5-GW/LacZ, Invitrogen, Grand Island, NY, USA) or luciferase (pSIN-Luc). Pluripotent authenticity and multilineage proficiency were validated in individual iPS cell clones by established in vitro (stemness markers expression, metabolic fingerprinting, and embryoid body differentiation), in vivo (teratoma formation) and in utero (diploid aggregation and contribution to organogenesis) criteria Folmes et al. 2011). Ultrastructure was examined by transmission electron microscopy (JEOL 1200 EXII, Jeol Ltd, Tokyo, Japan).

Targeted cell delivery into infarcted regions
Under 1-2% isoflurane anaesthesia, permanent ligation of the left anterior descending coronary artery was performed on male, 8-to 12-week-old C57BL/6 or athymic nude mice (Harlan Laboratories, Indianapolis, IN, USA) as described Behfar et al. 2010). Pain prophylaxis was implemented by an acetaminophen regimen (100-300 mg kg −1 in drinking water) 2 days prior to and 5 days after surgery. Post-ligation, infarcted mice (n = 56, 17 C57BL/6, 39 athymic nude) were randomized into vehicle-treated (25 athymic nude), fibroblast-treated (n = 16, 9 C57BL/6, 7 athymic nude), and iPS cell-treated (n = 15, 8 C57BL/6, 7 athymic nude) cohorts. Fibroblasts or derived iPS cells (200,000 cells per heart in 15 μl propagation media) were delivered into mapped peri-infarcted anterior walls of the left ventricle (LV) by epicardial injection (40,000 cells per site × 5 sites per heart) within 30 min following coronary ligation ( Fig. 1E and F). Cell dose was selected based on previous studies demonstrating that an intracardiac delivery of 200,000 pluripotent stem cells per heart was safe and sufficient to mediate repair (Yamada et al. 2008;Nelson et al. 2009). Immunocompetent hosts were free from uncontrolled growth up to 60 weeks following iPS cell delivery. In contrast, immunodeficient recipients developed teratoma within 4 weeks as previously reported ), which compromised speckle-tracking and haemodynamics surveillance (Fig. 2). Cohorts demonstrating aneurysmal formation with ejection fraction <25% immediately after infarction were excluded as typical ischaemic cardiac dyssynchrony is characterized by chronic disease progression and dyssynchronous wall motion defined as delayed and reduce peak contraction. Accordingly, functional, structural and electrical endpoints following cell therapy were acquired in an investigator-blinded fashion in immunocompetent hosts. Safety evaluation included daily observation, check of vital signs, electrocardiography, and cell tracking. Systemic histological evaluation upon autopsy was performed at Ultrastructure of fibroblasts (A) and derived induced pluripotent stem cells (iPS; B) in transmission electron microscopy demonstrating morphological changes from flat fusiform fibroblasts to smaller round iPS with a high nucleus/cytoplasm ratios. Alkaline phosphatase staining (C) and gene expression profiling (D) confirmed pluripotent reprogramming. E, numbers of animals per group are indicated in parentheses. F, long-axis radial strain maps deconvoluting infarction-induced regional deformation with superimposed sites of targeted stem cell injection. Upper panel, 2-dimensional view; lower panel, peak strain in 3-dimensional view; X, cell injection sites; R-R, R-R interval; 1, basal anterior-septum (Ant.sept.); 2, mid-Ant.sept.; 3, apical Ant.sept.; 4, apical posterior wall (Post.); 5, mid-Post.; 6, basal Post. J Physiol 591.17 1 month post-infarction (1 or 2 randomly selected animals in each cohorts), and at the end of the study.

Speckle-based deformation mapping and analysis
Regional and global cardiac dynamics, including contractility and synchrony, were deconvoluted by speckle-tracking echocardiography (VisualSonics). Cardiac cycles were acquired digitally from the parasternal long-axis and mid-ventricular short-axis views for assessment of radial, circumferential and longitudinal systolic strain/velocity, and time-to-peak systolic strain/velocity (Bauer et al. 2011;. The LV endocardium was mapped using 48 sampling points that divided the chamber into six segments. In long-axis, the basal anterior-septum, mid-anterior-septum, apical anterior-septum, basal posterior wall, mid-posterior wall and apical posterior segments were defined. In mid-ventricular short-axis, the anterior, anterior-septum, inferior-septum, inferior, posterior, and anterior-lateral segments were further delineated. Validation criteria included stable and continuous endocardial tracking throughout cardiac cycles with a heart rate >380 beats min −1 and a frame rate >180 s −1 (average heart rate of 478 ± 14 beats min −1 and frame rate of 219 ± 11 s −1 ). Ninety-five per cent of sampling points were successfully tracked under pre-established criteria, and further analysed for peak systolic strain/velocity and time-to-peak strain/velocity. Strain (ε) was defined as change in length during myocardial contraction and relaxation, and expressed as a percentage: ε = (L 1 -L 0 )/L 0 = L/L 0 , where L 0 is original length, L 1 is final length, and L is change in length (Sachdev et al. 2011). Tissue contraction patterns were expressed as negative strain values for longitudinal and circumferential motion, and positive values for radial strain. In each segment, peak systolic strain (%) and time-to-peak systolic strain (ms) were analysed. Patterns of abnormal ventricular contractility were classified according to systolic strain magnitude Figure 2. Immunocompetent status defines cell growth outcome Immunocompetent infarcted hearts were free from uncontrolled growth following iPS cell implantation as documented in vivo (echocardiography; A and B) and on autopsy (A and C) during the 60-week-long follow-up, in contrast to teratoma formation observed in immunodeficient hosts. In A: M, mass; LV, left ventricle; S, suture for coronary ligation. In B, data represent means ± SEM (n = 8 immunocompetent hearts: n = 7 immunodeficient hosts); * P < 0.05 versus immunocompetent.
(peak) and timing (initiation and peak of shortening). Specifically, dyssynchrony was defined as a pattern of reduced systolic strain magnitude, early opposite deflection and delayed time-to-peak systole; akinesis as minimal or no contractility with peak systole between −5% and 5%; and dyskinesis as ventricular systolic motion occurring opposite to contraction with peak systolic strain <−5% in radial and >5% in circumferential/longitudinal strain (Carasso et al. 2009). Intra-ventricular disparity was quantified by standard deviation of time-to-peak strain/velocity across segments. Stretch was defined as motion occurring in opposite direction to contraction. Systolic total stretch consisted of stretching preceded or not by shortening (De Boeck et al. 2009). Stretch-to-shortening ratio (%) was calculated as [(systolic total stretch)/(systolic total shortening)] × 100.

Statistical analysis
Data are presented as means ± SEM. Paired group analysis was performed using Student's t test or non-parametric Mann-Whitney U test. Two-way repeated-measures ANOVA was employed for comparison between groups over time (JMP 9, SAS Institute, Cary, NC, USA). Kaplan-Meier analysis with log-rank testing was applied for survival analysis. P < 0.05 was predetermined as significant.

Targeting ischaemic dyssynchrony by cell therapy
Advanced echocardiography, applied in vivo to murine hearts beating at physiological rates (387-560 beats min −1 ), enabled tracking of tissue speckle patterns (Fig. 3). Speckle tracking is less dependent on the angle of the ultrasound beam and passive wall motion of the heart, complementing more traditional 2-dimensional (2-D) and M-mode echocardiography (Anderson et al. 2008). Pre-infarction, systolic radial strain was positive and continuous throughout 48 sampling points of the LV endocardium, indicating homogeneous tissue contraction (Fig. 3A). Ligation of the left anterior descending coronary artery precipitated contractile deficit originating from the infarcted anterior-septum and apical segments (day 1; Fig. 3B), which progressively advanced into broader deformation and aberrant intra-ventricular disparity (days 10 and 30; Fig. 3C and D). Despite initial similarity in strain patterns between infarcted hearts with or without cell therapy ( Fig. 3B and F), local delivery of iPS cells into mapped infarcted areas (Fig. 1F) gradually salvaged wall motion (Fig. 3G), re-introducing normokinesis within 30 days post-infarction ( Fig. 3E-H). Peak systolic strain was >30% in all segments of iPS cell-treated ventricles (Fig. 3H), in contrast to the <5% level that persisted in half of the segments within untreated ventricles (Fig. 3D). Untreated anterior and apical segments demonstrated loss of contractility with basal and mid-posterior walls manifesting intra-ventricular conduction delay within 30 days post-infarction (Fig. 4A). Regional iPS cell intervention restored contractility throughout the mapped segments (Fig. 4B). Subtraction maps, constructed as deviations of strain values from days 1 to 30 post-infarction, pinpointed iPS cell-dependent contractile recovery within the infarcted anterior-septum and prevention of malfunction in the non-infarcted posterior wall ( Fig. 4C and D). Thus, the infarcted region is an epicentre of cardiac dyssynchrony and represents a responsive target to iPS cell-based intervention.

Cell-mediated reversal of initial dysfunction translates into sustained benefit
Over the 3 months follow-up, iPS cell-based intervention produced a stable benefit on tissue contractility across ventricular segments documented in 2-D, 3-D, and oblique views of radial strain (Fig. 5). In contrast, transplantation of fibroblasts demonstrated persistent dysfunction in infarcted zones and paradoxical hypercontractility in non-infarcted areas (Fig. 5A-C). Within the primary infarcted area, namely the mid-anterior-septum region, peak radial strain from the parasternal long-axis was 16 ± 2% in fibroblast-treated hearts (n = 6), yet it improved to 41 ± 4% in iPS cell-treated counterparts (n = 6, P < 0.01; Fig. 6A). Regional improvement achieved by iPS cell therapy translated into recovery of LV radial strain, which averaged 30 ± 4% across all segments in the iPS cell-treated group (n = 6), significantly higher than 17 ± 2% measured in the fibroblast-treated cohort (n = 6, P < 0.05; Fig. 6B).

J Physiol 591.17
The superior outcome of iPS cell therapy, over fibroblast treatment, was validated by complementary spatial deconvolution. Specifically, longitudinal ( Fig. 6C and D), short-axis radial ( Fig. 6E and F), and circumferential ( Fig. 6G and H) strains all demonstrated significantly improved contractility within infarcted regions leading to functional restitution across the ventricle in iPS cell-treated, but not fibroblast-treated, hearts. Of note, low strain values were equivalent between fibroblast-treated and -untreated cohorts (Fig. 6A-H), indicating absence of detrimental effects following fibroblast treatment. Thus, iPS cell transplantation rescues myocardial contractility throughout the long-term follow-up period, offering sustained benefit.

Cell therapy synchronizes wall motions between infarcted and non-infarcted areas
The difference in myocardial kinesis between infarcted and non-infarcted areas provoked, in the absence of iPS cell therapy, a pronounced disparity of contractile timing (Fig. 6I). Time-to-peak strain was markedly prolonged within segments transitioning from the non-contractile anterior-lateral wall to the contractile posterior wall with fibroblast treatment of infarcted hearts, in contrast to an even time-to-peak distribution afforded by iPS cell treatment (Fig. 6J). Similarly in long-axis, iPS cell transplantation, but not fibroblast therapy, eliminated the delayed contraction and restored a uniform wall motion across LV segments (Fig. 7). Abnormal strain patterns (aberrant magnitude, timing, and direction of ventricular wall motion), dysfunctional stretch (degree of misdirected contraction), and increase in intra-ventricular delay of time-to-peak strain (heterogeneous contraction timing), which characterized myocardial infarction in the absence of stem cell therapy (Fig. 7D-F), were prevented or normalized in response to iPS cell treatment (Fig. 7G-J). Moreover, the stretch-to-shortening ratio significantly decreased (15 ± 4% in fibroblast, n = 6; 2 ± 1% in iPS, n = 6, P < 0.05; Fig. 7K ), while standard deviation of time-to-peak systolic strain stabilized following iPS cell   therapy (16 ± 3 ms in fibroblast, n = 6; 9 ± 1 ms in iPS, n = 6, P < 0.05; Fig. 7L). Vector velocity analysis validated the iPS cell-mediated rescue of myocardial contractility and synchrony (Fig. 8). Recovery of contractility matched cell engraftment following iPS cell delivery ( Fig. 8A-C), and was sustained throughout follow-up (Fig. 8D-F). Thus, by reducing the functional heterogeneity in infarcted and non-infarcted areas, iPS cell therapy prevents mechanical dyssynchrony post-infarction.

Discussion
Therapies that incorporate device-based resynchronization in the management of heart failure are integral to post-infarction care. Indications for device-based resynchronization pacing are chronic, advanced stages of disease diagnosed through global cardiac and systemic evaluation. Yet, practice guidelines do not specify the underlying pathophysiological properties of the failing myocardium amenable to resynchronization (Daubert et al. 2012). Despite observed benefit, current practice with pacing regimens indicates a significant portion of non-responders among treated individuals. In particular, scar formation post-infarction is a recognized risk for unfavourable response to device-based cardiac pacing, which critically relies on viable tissue (Mele et al. 2009;Riedlbauchová et al. 2009;Khan et al. 2012). Early interventions that would focus on the origin of disease are thus needed to complement the existing armamentarium for adequate cardiac resynchronization. Accordingly, this study utilized speckle-tracking echocardiography to pinpoint the epicentre of dyssynchrony and used bioengineered pluripotent stem cells for tissue repair. Delivery of iPS cells in the acute phase of infarction selectively eliminated dysfunctional substrates, and achieved long-term resynchronization at the whole organ level. Stem cell-based resynchrony thus emerges as a biotherapeutic strategy to address the primary defects in myocardial pathodynamics underlying cardiac dyssynchrony post-infarction.

Mechanical dyssynchrony post-infarction resolved in vivo by deformation deconvolution
Even following successful reperfusion mechanical dyssynchrony commonly develops within 48 h post-infarction, and is a predictor of adverse ventricular remodelling (Mollema et al. 2007;Nucifora et al. 2010). Heterogeneous wall motion imposes increased wall stress and triggers neuro-hormonal activation, resulting in pathological remodelling with pump failure and poor outcome (Auricchio & Prinzen, 2008). By fingerprinting tissue speckle patterns, speckle-based strain/velocity analysis offers an in vivo non-invasive insight into contractile dynamics (Popović et al. 2007). Speckle-based imaging of tissue deformation pinpoints the failing region, and resolves the collapse in cardiac force generation ). Accordingly, here, high-resolution speckle-tracking echocardiography unmasked the initiation of the disease process. Specifically, the present study identified the transition from the initial focal insult to global dyssynchrony within infarcted murine ventricles. At 3 months, the assessed coronary ligation model displayed dyssynchrony and discoordination of wall motion, QRS widening, low ejection fraction, and chamber dilatation with wall thinning, recapitulating clinical cardiomyopathy traits (Daubert et al. 2012). In this way, the murine infarction model used herein combined with advanced imaging provided a relevant experimental foundation to track the onset, progression, and mechanisms of cardiac dyssyncrhony post-infarction.

iPS cell-based resynchronization underlies benefit post-infarction
Due to a limited capacity for self-renewal, the heart is vulnerable to ischaemic stress and susceptible to organ failure. Discovery of stem cell populations that exhibit the potential to differentiate into specialized tissue types has provided the foundation for cell-based regenerative medicine, boosting innate mechanisms of repair (Bartunek et al. 2010;Janssens, 2010). iPS cells harbour a recognized capacity to generate functional cardiac-like beating syncytia expressing contractile proteins and ion channel sets responsive to excitation inputs in vitro (Zhang et al. 2009;Moretti et al. 2010;Nelson et al. 2010). Moreover, in vivo, iPS cell treatment achieves multilineage tissue reconstruction post-injury Mauritz et al. 2011;Singla et al. 2011). While a number of cell properties may contribute to survival post-transplantation, iPS cells rely on oxygen-independent glycolytic metabolism, providing survival advantage in a low-oxygen environment (Folmes et al. 2013). Success of cell therapy for myocardial infarction has been evaluated by cell fate mapping and rescue of heart failure (Wollert & Drexler, 2010). In this study, in vivo imaging and histological assessment validated iPS cell engraftment and differentiation, as Figure 9. iPS cell therapy improves ventricular conduction and performance post-infarction iPS cell implantation, but not parental fibroblasts (Fib), eliminated cardiac dyssynchrony traits, i.e. wide QRS complex and ventricular dilatation with reduced contractility (A). B-D, benefits of iPS cell treatment over untreated cohorts were significant in this randomized and investigator-blinded study. In A: Ant.sept., anterior-septum; LV, left ventricle; Post., posterior wall; green solid/dotted arrow, LV diastolic/systolic dimension; yellow solid/dotted arrow, LV diastolic/systolic wall thickness. In B-D: d, day; m, months; data represent means ± SEM (n = 25 in vehicle (Veh), n = 9 in Fib, n = 8 in iPS); dotted lines, pre-infarction (Pre) 95% confidence interval (CI); + P < 0.05 versus Pre; * P < 0.05 versus Fib. J Physiol 591.17 well as endogenous cell cycle activation within the diseased host heart, and demonstrated reduced fibrosis post-infarction. Moreover, iPS cell treatment restored ejection fraction and prevented ventricular dilatation. Cardiac regional properties are a valuable readout in assessing cell therapies (Menasche, 2011;Petersen et al. 2011). Here, iPS cells were locally delivered, within 30 min post-infarction, into hypokinetic or akinetic areas defined by speckle-based strain mapping. Prospective speckle-tracking echocardiography revealed that iPS cell implantation selectively rescued contractility and dyssynchrony in the infarcted regions, leading to global cardiac resynchronization with reverse remodelling. Multiparametric speckle-based analysis demonstrated that recovery of the infarcted anterior-septum preceded rescue of the cardiomyopathic phenotype, suggesting repair of the dyssynchrony substrate as a mechanism for long-term benefit of the iPS cell intervention. While histological assessments identified cell fate within the host environment, in vivo wall motion analysis through speckle-tracking echocardiography demonstrated reestablishment of myo-cardial mechanical properties. In this regard, correction of cardiac wall motion offers an integrated readout of myocardial function achieved by tissue repair through differentiation into cardiomyocytes, vasculature and/or paracrine effects, cell fusion, and the involvement of an innate regenerative response. With no evidence of uncontrolled cell growth or mortality at follow-up in the setting of an allogeneic model, the present study provides initial preclinical demonstration of safe and feasible iPS cell-based resynchronization in the failing infarcted heart. Translation of this proof-of-concept study will require optimization of the stem cell source, methodology of nuclear reprogramming, purging of pluripotent stem cells and securing lineage specific derivatives to eliminate risk of uncontrolled growth, followed by optimization of dose and delivery methods, and tissue implantation. In addition to the differentiation capacity of stem cells, cell-host interaction, immune tolerance and inflammation may all affect cell survival/growth post-transplantation (Nussbaum et al. 2007). Potential applications of stem cell-based resynchronization include non-responders to current optimal therapies, and prophylactic early intervention Histological assessment demonstrated that iPS cells reduced scar formation (A-C), differentiated and activated cell cycle within the host environment (D-F), and ultimately averted ventricular dilatation, preventing akinesis and wall thinning post-infarction (G-I). Stem cell intervention translated into reverse cardiac remodelling in vivo (J and K). Fib, fibroblasts; LV, left ventricle; RV, right ventricle; data represent means ± SEM (n = 25 animals in vehicle (Veh), n = 9 animals in Fib, n = 8 animals in iPS); dotted lines, pre-infarction (Pre) 95% confidence interval (CI); + P < 0.05 versus Pre; * P < 0.05 versus Fib. In D and E: DAPI, 4 ,6 -diamidino-2-phenylindole; β-gal, β-galactosidase. In J: white arrow head, suture (S) for coronary ligation; white arrow, LV dimension; yellow arrow, wall thickness of LV free wall; scale bars, 5 mm. In K: d, day; m, months.
for high-risk groups in heart failure. The concept of biological cell-based resynchronization, as opposed to traditional device-based resynchronization, is supported by recent clinical and preclinical evidence (Chang et al. 2008;Herbots et al. 2009;van Ramshorst et al. 2009;Bonios et al. 2011;Pokushalov et al. 2011). Due to complexity in the regulation of myocardial mechanics, as well as to the study design focusing on prospective in vivo imaging, the relationship of injury, regeneration and resynchronization processes is yet to be fully delineated. Further studies regarding the mechanisms of how to control cardiac wall motion and reconnect cellular and organ physiology will contribute to the establishment of individualized therapeutic protocols in cardiac dyssynchrony post-infarction Gorcsan & Prinzen, 2012;Smith et al. 2012).

Conclusions
Wall motion analysis provides physiological insights into cardiac force generation in vivo. Here, high-fidelity speckle-tracking imaging applied to a murine infarction model deconvoluted the evolution of mechanical dyssynchrony and assessed long-term responsiveness to a targeted regenerative intervention. Delivered locally within post-ischaemic dyssynchronous regions, iPS cell therapy synchronized the infarcted tissue and prevented development of refractory ischaemic cardiomyopathy, avoiding organ failure. Biological resynchronization attained through bioengineered stem cell transplantation introduces thereby a novel strategy to preserve cardiac dynamics through tissue repair strategies.