Genetic variations of β-MYH7 in hypertrophic cardiomyopathy and dilated cardiomyopathy

CONTEXT: Hypertrophic cardiomyopathy (HCM) is known to be manifested by mutations in 12 sarcomeric genes and dilated cardiomyopathy (DCM) is known to manifest due to cytoskeletal mutations. Studies have revealed that sarcomeric mutations can also lead to DCM. Therefore, in the present study, we have made an attempt to compare and analyze the genetic variations of beta-myosin heavy chain gene (β-MYH7), which are interestingly found to be common in both HCM and DCM. The underlying pathophysiological mechanism leading to two different phenotypes has been discussed in this study. Till date, about 186 and 73 different mutations have been reported in HCM and DCM, respectively, with respect to this gene. AIM: The screening of β-MYH7 gene in both HCM and DCM has revealed some common genetic variations. The aim of the present study is to understand the pathophysiological mechanism underlying the manifestation of two different phenotypes. MATERIALS AND METHODS: 100 controls, 95 HCM and 97 DCM samples were collected. Genomic DNA was extracted following rapid nonenzymatic method as described by Lahiri and Nurnberger (1991), and the extracted DNA was later subjected to polymerase chain reaction (PCR) based single stranded conformation polymorphism (SSCP) analysis to identify single nucleotide polymorphism (SNP)s/mutations associated with the diseased phenotypes. RESULTS AND CONCLUSION: Similar variations were observed in β-MYH7 exons 7, 12, 19 and 20 in both HCM and DCM. This could be attributed to impaired energy compromise, or to dose effect of the mutant protein, or to even environmental factors/modifier gene effects wherein an HCM could progress to a DCM phenotype affecting both right and left ventricles, leading to heart failure.


Introduction
Cardiomyopathy is a disease of the heart muscle, associated with cardiac dysfunction.It is classified as hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy (DCM), restrictive cardiomyopathy (RCM) and arrhythmogenic right ventricular dysplasia (ARVD/C).HCM is characterized by hypertrophy of the left ventricle with the predominant involvement of the interventricular septum (diastolic dysfunction).
Its prevalence is found to be 1 in 500. [1,2]DCM is characterized by cardiac dilatation, predominantly of the left ventricle, and weakening of the heart muscle beyond repair, resulting in reduced contractile function and inefficient pumping of the heart (systolic dysfunction).It accounts for 70-80% of all cases of cardiomyopathy.
Till date, about 186 and 73 different mutations have been identified in HCM and DCM, respectively, with respect to β-MYH7 gene.Since most of the SNPs/ mutations have been reported in this gene, the present study includes the screening of this gene for genetic variations in both the diseases to evaluate the underlying pathophysiological mechanisms leading to either hypertrophy or dilatation. [3,4]mmon genetic variations in exons 7, 12, 19 and 20 of this gene were found in both the HCM and DCM, which emphasizes the role of genetic modifiers and environmental factors along with impaired energy DOI: 10.4103/0971-6866.69348compromise and dosage gene effects of the protein leading to hypertrophy or dilatation eventually resulting in heart failure.Genomic DNA was extracted by rapid nonenzymatic method as described by Lahiri and Nurnburger. [5]DNA was amplified based on the primer sequences available on Siedman's database on the website http://genetics.med.harvard.edu/~seidman/cg3/genes/MYH7_exons.html.

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Polymerase chain reaction (PCR) was carried out in 0.2 ml tubes containing 100 ng of genomic DNA, 50 pmol each of forward and reverse primers, 0.5-1 U of Taq DNA polymerase enzyme, 200 μM of dNTPs, 1× PCR buffer and water to make up the volume to 25 μl.Initial denaturation was carried out at 95°C for 3 min, followed by a denaturation step at 95°C for 30 s.The annealing temperature varied from 54°C to 65°C based on the exon for 30 s and an extension at 72°C for 1 min.
A final extension step of 72°C was for 2 min at the end of the reaction.
The amplified DNA samples were subjected to SSCP analysis on nondenaturing/polyacryalamide gels.
da et al. 2009 (communicated)].The list of SNPs/ mutations which are found to be common in both the diseased types is given in Table 1. [6]tational screening of MYH7 gene in HCM revealed five SNPs in exons 7, 12, 19 and 20 of which three were heterozygous and two were homozygous, [Figure 1, 2, 4] whereas the same SNPs were found to be homozygous [Figure 2] in DCM [Figure 3] sample, revealing the dose effect of the protein with the gross anatomical variations in the ventricles leading to heart failure in DCM cases.

Discussion
Myosin is the most conserved and a major contractile protein, which helps in force generation in the myocardium.
Since the maximum number of mutations or SNPs are found to be in MYH7, we made an attempt to screen this gene in both HCM and DCM.] Phenotypic plasticity Genotype-phenotype correlations can be best explained by phenotypic plasticity where mutations/ variations in the same gene and even the same mutation/ variation in a different background cause disparate phenotypes. [10]In the case of cardiomyopathies, such phenotypic plasticity was best illustrated by Dr.
Seidman's group, who reported that mutations in the MYH7 and TNNT2, encoding beta-myosin heavy chain and cardiac troponin T, respectively, could cause either HCM or DCM, the opposite ends of the spectrum of phenotypic responses of the heart to injury, stress or mutations. [11]terations in myofibrillar Ca 2+ Same variations leading to different diseased phenotypes can also be explained by the energy compromise and alterations in myofibrillar Ca 2+ sensitivity pathways.Impaired myofibrillar Ca 2+ sensitivity and force transmission of cytoskeletal protein variations could result in ventricular dilatation in DCM.On the other hand, sensitization to Ca 2+ is likely to promote systolic function in HCM but impaires diastolic function due to an increase in cytosolic free Ca 2+ . [12,13]A study on Indians by Taranjit Singh et al. [14] with a similar approach, in which MYH7 mutations were found in both HCM and DCM, further supports our data and reconfirms the fact indicating that there is a wide genetic and phenotypic heterogeneity of HCM and DCM in our population similar to that reported for other ethnic populations such as Caucasians and 7][18][19][20] In addition to the above discussed reasons, environmental factors or modifier gene effect could also be playing a significant role in diverse phenotypes.
Therefore, our results have reinforced the fact that the progression of hypertrophy to dilatation could finally lead to heart failure.

Genotype beta-phenotype correlation
Gross abnormalities in β-MYH7 might have a little pathogenic potential in the heterozygous state and minor The clinical utility of such findings can be correlated to poor prognosis and sudden cardiac deaths in DCM compared to HCM, based on the dosage effect.A report of Reena et al. [6] on genetic testing of β-MYH7 in a large kindred helped in the prediction of asymptomatic, presymptomatic and symptomatic individuals based on the mutation R870H and identified the genotype phenotype correlation being established. [6]SNPs/ mutations have been detected in other sarcomeric genes in patients not harboring β-MYH7, revealing genetic heterogeneity of the condition.

Conclusion
In the present study, common genetic variations were found in MYH7 gene in both HCM and DCM samples This could be attributed to impaired energy compromise, or to dose effect of the mutant protein, or to even environmental factors/modifier gene effects responsible for an HCM to progress to a DCM phenotype in which both right and left ventricles are affected culminating into heart failure.
a r d i o m y o p a t h i e s ( H C M a n d D C M ) w e r e diagnosed by physical examination, echocardiogram, electrocardiogram and magnetic resonance imaging.Primary DCMs were diagnosed through coronary angiogram, wherein one can rule out the possibility of an underlying myocardial infarction/coronary artery disease, valvular disease and/or other systemic disorders.Blood was collected from 100 healthy blood donors with no history of cardiac disorders and these samples were treated as controls.Blood samples from 97 DCM cases comprising 20 juvenile, 38 primary and 39 secondary cases, and 95 HCM samples were also collected from cardiology units of CARE Hospitals, Mahavir hospitals and Niloufer hospitals, Hyderabad.Informed written consent was obtained from patients in accordance with the study protocol along with the institutional ethics committee's approval to carry out the above study.

Figure 1 :Figure 4 :Figure 2 :Figure 3 :
Figure 1: SSCP analysis of exon 19 in HCM abnormalities may have a causative role proving dominant negative effect of the mutated protein.Polymorphisms that are associated with the disease condition could be present in the regulatory region of the gene and potentially affect the expression of the genes and hence may be relevant to disease susceptibility.Heterozygous condition is associated with mild hypertrophy (with or without obstruction), relatively good prognosis, agedependent penetrance and longer life span, whereas the homozygous state is associated with an increased risk of the disease at an early age.The heterozygous SNPs are regulatory in nature acting as susceptiblity alleles.Early onset of the disease is predicted in homozygous state with predominant left ventricle and left auricle dilatations with Ejection Fraction (EF) (>40%) along with global hypokinesia in DCM.Hypertrophy in homozygous condition may progress to DCM condition and eventually leads to heart failure.