Solid State Characterization of Domperidone : Hydroxypropyl-β-Cyclodextrin Inclusion Complex

The purpose of the present study was to prepare inclusion complex of domperidone with hydroxylpropyl-βcyclodextrin in order improved the solubility and hence to increase dissolution of domperidone. An effect of concentration of hydroxylpropyl-β-cyclodextrin on the aqueous solubility of domperidone was determined by phasesolubility method. The aqueous solubility of domperidone increased as a function of hydroxylpropyl-β-cyclodextrin concentration, showing AL type diagram. Solid domperidone/hydroxylpropyl-β-cyclodextrin complex was prepared in ratio 1:1 by ultrasonication and kneading method. Solid state inclusion complex was characterized by FTIR, powder X-ray diffraction and differential-scanning calorimetry techniques. FTIR studies showed intactness of drug in complex whereas powder diffraction studies showed that hydroxylpropyl-β-cyclodextrin complex was amorphous. Solubility studies showed that complexation increased domperidone solubility as compared to pure drug in 0.1M hydrochloric acid and distilled water. Drug content confi rms that ultrasonication is one of the effi cient methods to prepare inclusion complex. Dissolution data of inclusion complexes also indicated that there is 1.4 folds increase in dissolution as compared to pure drug and was observed in case of inclusion complexes prepared by ultrasonication.

Domperidone (DPD) is a widely used antiemetic, poorly water soluble drug, erratically absorbed in stomach with several dissolution related problems leading to poor bioavailability [1] .Complexation has been frequently used to increase the aqueous solubility and dissolution rate of water insoluble and slightly soluble drugs in an effort to increase oral bioavailability.However, in certain instances, this approach can also be used to increase drug stability (13-cis-retinoic [2] , disoxaril [3] ), control drug release rate (nicardipine [4] , vinpocentine [5] , fentanyl [6] ), improve organoleptic properties and maximize the gastrointestinal tolerance by reducing drug irritation after oral administration.Amongst different β-cyclodextrins (β-CDs), hydroxylpropylβ-cyclodextrin (HP-β-CD) was selected considering its greater water solubility of ~ 45% w/v over β-cyclodextrin (~ 2% w/v) [7] .
In our work, inclusion complexes have been prepared of HP-β-CD and DPD in solid state.Complex formation was confi rmed using a variety of analytical techniques as well as dissolution and solubility characteristics.The aim of this work was to evaluate the effect of the different preparation methods on the physicochemical properties of DPD in the binary mixtures.Knowledge of the interaction between the DPD and the cyclodextrin derivative may provide useful information on the development of pharmaceutical forms for oral administration.Domperidone (IPCA Laboratories, Ratlam, India), and HP-β-CD (Roquette Fereres, France) were received as gift sample.All other chemicals used in the study were of analytical grade and used as received.
Phase solubility studies were performed according to the method reported by Higuchi and Connors [8,9] .An excess amount of DPD was added to 10 ml of distilled water containing increasing concentration HPβ-CD solution (0.01-0.1 M) in 10 ml screw capped bottles.The contents were stirred for 72 h at 37 ° on a rotary fl ask shaker.After equilibrium, the samples were filtered through Whatman filter paper No. 42 and absorbances were recorded at 284 nm.
The apparent stability constant was calculated from the initial straight portion of the phase solubility diagram using the Eqn.K1:1=slope/ So(1-slope)×M-1, where, 'So' is solubility of drug without cyclodextrin, M is molar concentration, K is apparent stability constant and slope is calculated from regression Eqn.
Inclusion complex was prepared according to method described by Liu, et al [8] .DPD and the HP-β-CD (USHP1) were weighed (1:1 molar ratio) and transferred to beaker containing equivolume mixture alcohol-water, sufficient solvent was added to maintained paste like consistency.The resulting paste was then sonicated for 6 h.Throughout the ultrasonication, a paste like consistency was maintained using alcohol-water.Then it was dried in an oven at 50° for 24 h.The dried complexes were passed through sieve No. 100.The prepared complexes were stored in glass vials and used for further studies [10] .
DPD and HP-β-CD (KNHP1) were weighed (1:1 molar ratio) and transferred to a mortar and kneaded for 45 min, using equivolume alcohol-water mixture, suffi cient solvent was added to maintain a paste like consistency.The resulting paste was then dried in the oven at 50 ° for 24 h.The dried complexes were ground in a mortar for 2 min and passed through sieve No. 100.The prepared complexes were stored in glass vials and used for further studies [11] .Physical mixtures [12] were prepared by simple blending of DPD and HP-β-CD in a 1:1 molar ratio uniformly in a mortar.
The DSC study was carried out with Mettler DSC 30S, Mettler Toledo India Pvt. Ltd., Switzerland, using crucible Al 40 µl, at of 10 °/min heating rate, under nitrogen environment.The temperature range used was 0-400 °.Powder XRD was carried out with X-ray powder diffraction system, PANlytical Spectris Pvt. Ltd., Singapore using copper target, a voltage of 40 Kv and a current of 30 mA.The scanning was done over 2θ range of 5° to 60°.The FT-IR spectra of pure drug, pure M-β-CD, physical mixtures and inclusion complex were taken by preparing KBr pellets using pressure 6-8 tons, die size 13mm and scan between 4000-500 cm -1 .
The percent drug content of each inclusion complex was determined using powder equivalent to 10 mg DPD and was dissolved in 20 ml 0.1M HCl using the mechanical shaker for 20 min.and to the solution obtained 0.1M HCl was added, volume was made up to 50 ml.The solution was then fi ltered through Whatman filter paper No.42 and required dilutions were being made and absorbance was taken at 284.20 nm.
Inclusion complexes equivalent to 10 mg of DPD was taken and to this 10 ml of the respective medium was being added in 100 ml stoppered volumetric flasks and shaken for 24 h at room temperature (25 ) on a mechanical shaker.After 24 h, samples were fi ltered through Whatman fi lter paper No.42 and aliquots were suitably diluted for estimating solubility [13] .
Dissolution studies on pure drug and inclusion complexes (equivalent to 10 mg of drug) were performed using USP II (Rotating paddle type) at 100 rpm.Hydrochloric acid at pH 1.2 (0.1 M 900 ml) maintained at 37±0.5 ° was used as dissolution media.During dissolution study 10 ml aliquot was withdrawn at different time intervals of 5, 10, 15 up to 60 min.and same was replaced with equal volume of fresh medium.The withdrawn samples were fi ltered through Whatman fi lter paper No. 42 and absorbances were measured at 284.20 nm.Cumulative percent drug dissolved was found out at each time interval and graph was plotted between cumulative % drug dissolved and time in min.
The inclusion complex (USM1) was selected as optimized inclusion complex and stability study was carried out at [13] 25±2 ° at 75±5% RH and 40±2 ° at 75±5% RH, for a period of 3 months.The inclusion complexes were placed in amber coloured bottles and put at above specifi ed conditions for 3 months.After every month inclusion complexes were analyzed for drug content.
From the phase solubility study it was observed that HP-β-CD showed A L type phase solubility curve indicated improved solubility.Solubility of DPD increased in a linear fashion with increased concentration of HP-β-CD (R 2 = 0.990) and showed A L type phase solubility curve indicating that soluble complexes were formed and no precipitation was observed (fig.1).This fact is well supported by Challa et al [14] .The stability constant (Ks) of the 1:1 complex of DPD with H-β-CD was calculated from slope of straight line in A L type solubility diagram and was found to be following 87.33 M -1 .K S values obtained is less but adequate for the formation of inclusion complexes which may contribute improving the bioavailability of poorly water soluble drugs.
The FT-IR of pure DPD is characterized by N-H stretching: 3122 cm -1 , C=O stretching: 1714.60 cm -1 , indicating the presence of -CONH group.
The X-Ray diffraction pattern of DPD exhibited sharp, highly intense and less diffused peaks indicating the crystalline nature of drug.The X-Ray diffraction pattern of physical mixture of DPD with HP-β-CD was simply a superimposition of each component with peaks of both DPD and carriers however with lower intensity.The kneaded and sonicated inclusion complexes showed less intense and highly diffused peaks of drug which was very poor in reflections which testified to a reduced ordering of crystal lattice indicating formation of amorphous solid state (fig.3).The extent of crystallinity influences the dissolution of a drug.An amorphous, less crystalline and metastable form as compared to pure drug dissolves at a faster rate because of high internal energy and greater molecular motion which enhance the thermodynamic property as compared to crystalline materials [14] .In the prepared inclusion complexes there was a reduction in crystallinity of the drug as compared to pure sample which refl ects that the drug is dispersed in the polymer and hence increases in the solubility as compared to pure drug.The thermal curve of In all the inclusion the drug melting endotherm broaden and shifted to lower temperature passing from physical mixture (T peak =248.7°,∆H=39.3J/g) to kneaded 1:1 (242.9°,∆H=35.5 J/g) to ultrasonication 1:1 (T peak =240.9°,∆H=14.9J/g).In all the inclusion complexes, the drug endothermal effect further broadens and was almost hidden by the dehydration band of the carrier and it fi nally disappeared in the ultrasonication 1:1 (fi g. 4).This last phenomenon is attributable to both, inclusion complexes formation and/or drug amorphization Mura et al [15] .
Drug content of all inclusion complexes were in the range of 78.94-88.43%.This indicates the proper loading of drug in inclusion complexes and effectiveness of kneading method and ultrasonication.The solubility of all inclusion complexes was studied in distilled water and 0.1M HCl.The data indicated (Table 1) that solubility increased in all cases but highest increase was found in inclusion complexes prepared by ultrasonication.
The mean dissolution curve of DPD from various binary systems with CD's is present in fig. 5.It is evident at a glance that all system with CD's exhibited better dissolution properties than pure drug alone.Statistically significant differences in term of dissolution were found in all the DPD with HP-β-CD refl ecting stronger interaction.The greater ability of HP-β-CD in DPD amorphization could explain the better dissolution properties of the drug.As for the influence of the preparation method, the greatest improvement of drug dissolution was obtained with ultrasonication product, followed in order by kneading and finally by physical mixture.The increased dissolution rate (physical mixture) is attributable both to improvement in drug wettability and to formation of readily soluble complexes in dissolution medium.Further improvement obtained with kneading and ultrasonication could be explain by both the more intimate contact between drug and carrier and the decrease of drug crystallinity, as well as a phenomenon of at least partial drug inclusion complexation.On the contrary, the influence of the preparation method was clearly more marked in case of product with HP-β-CD, where kneaded and ultrasonication product showed an increase in dissolution effi ciency of 90 or 110%, in comparison to corresponding physical mixture.The best performance of these product seemed to confirm that drug inclusion complexation occurred substantially only in such systems, thus allowing to obtain the highest dissolution improvement.
Dissolution data of inclusion complexes also indicated that there is increase in dissolution as compared to pure drug and maximum increase was observed in case of inclusion complexes prepared by ultrasonication.Results of stability study indicated that the inclusion complex (USHP1) was stable and there was no signifi cant changes observed in the drug content (P>0.05).

Walode,
et al.: HPTLC Determination and Content Uniformity Test for Rosiglitazone A simple, rapid, precise and economical high performance thin layer chromatographic method has been developed and validated for determination of rosiglitazone in its tablet dosage form using caffeine as an internal standard.It was performed on silica gel 60 GF 254 thin layer chromatographic plates as a stationary phase using mobile phase me thanol:toluene:chloroform:triethylamine (1:8:0.5:0.5 v/v/v/v) and the detection was carried out in the absorbance mode at 264 nm showing R f value 0.31 for rosiglitazone and 0.52 for caffeine.The linear regression data curve shows good linear relationship in the concentration range 1.0-7.0µg/µl.The content uniformity test was carried out as per USP specifi cation of the content uniformity test of 85-115%.The percent drug estimated of rosiglitazone from two different marketed formulations were found to be in the range 99.83-100.21.The recovery of drugs was carried out by standard addition method were found to be 100.21±1.06 and 100.04±0.30by height and area