Simultaneous Spectrophotometric Determination of Drotaverine Hydrochloride and Paracetamol in Tablet

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Standard gift samples of drotaverine hydrochloride and paracetamol were procured from Vishnu Chemicals Ltd., Hyderabad.Combined drotaverine hydrochloride and paracetamol tablets (Label claim 80 mg DRO and 500 mg PAR) were purchased from local market.A Shimadzu UV/Vis double beam spectrophotometer, model 1700, with matched quartz cells corresponding to 1 cm pathlength and spectral bandwidth of 2 nm was used for performing the analysis.Methanol AR grade and distilled water were used as solvents in the study.
The stock solutions (100 μg/ml) of DRO and PAR were prepared separately by dissolving accurately about 10 mg of each drug in 25 ml methanol (AR grade) in 100 ml volumetric flasks and making up the volume with distilled water.Working standard solutions of DRO and PAR were prepared separately from standard stock solution.These solutions were scanned in the spectrum mode from 400.0 to 200.0 nm.The maximum absorbance of DRO and PAR was observed at 303.5 and 243.5 nm, respectively.The linearity of DRO and PAR was found to be in the concentration ranges of 5-50 μg/ml and 5-60 μg/ ml, respectively, at their respective maximas.The coeffi cients of correlation were found to be 0.9992 for DRO and 0.9995 for PAR.
In the Q analysis method, absorbances were measured at two wavelengths, one being the isoabsorptive point and other being the wavelength of maximum absorption of one of the two components.From overlain spectra of DRO and PAR (fig.1), absorbances were measured at the selected wavelengths, 277.0 nm (isoabsorptive point) and 243.5 nm (λmax of PAR).Appropriate dilutions were made to obtain mixed standard solutions in Beer-Lamberts' range for DRO and PAR in the ratio of 2:12.5 from 4, 8 μg/ml of DRO and 25, 50 μg/ml of PAR, respectively and scanned in the spectrum mode from 400.0 to 200.0 nm.The absorbances of mixed standards were measured at selected wavelengths.The concentration of each component present in mixed standard was calculated by mathematical treatment of the following mentioned equations.For drotaverine, where C 1 is concentration of DRO, C 2 is concentration of PAR, A 1 is absorbance of sample at isoabsorptive wavelength 277.0 nm and a 1 (0.0142) , a 2 (0.0143) are absorptivities of DRO and PAR at isoabsorptive wavelength 277.0 nm, respectively.Q x is the absorptivity of DRO at 243.5 nm/absorptivity of DRO at 277.0 nm, Q y is the ratio of absorptivity of PAR at 243.5 nm/absorptivity of PAR at 277.0 nm and Q m is the ratio of absorptivity of sample solution at 243.5 nm/absorptivity of sample solution at 277.0 nm.
In the fi rst order derivative method, solutions of 10 µg/ml of DRO and PAR were prepared separately.Both the solutions were scanned in the spectrum mode from 400.0 to 200.0 nm.The absorption spectra thus obtained were derivatized from fi rst to fourth order.First order derivative (n=1) was selected for analysis of both the drugs.The zero crossing wavelengths, 303.5 nm and 243.5 nm were selected for DRO (fig.2) and PAR (fig.3), respectively.Working standard solutions of DRO and PAR were prepared separately and scanned in the spectrum mode from 400.0 to 200.0 nm.The absorption spectra obtained were derivatized to obtain fi rst order derivative spectra.The absorbances of DRO and PAR were measured at 243.5 and 303.5 nm, respectively and working calibration curves of both the drugs were plotted separately.The coeffi cients of correlation were For determination of both drugs from commercial formulations, twenty tablets were weighed and crushed to obtain fi ne powder.The powder sample equivalent to 4 mg of DRO and 25 mg of PAR was weighed and transferred to 100 ml volumetric fl ask.The drug content was dissolved in 25 ml of methanol AR grade, and was kept in ultrasonicator for 30 min.Finally, the volume was made up to the mark with distilled water.The solution was filtered through Whatmann filter paper No. 41.The fi ltrate was further diluted to obtain mixed sample solutions in Beer-Lamberts' range for each drug in the ratio of 2:12.5 from 4 and 8 μg/ml of DRO and 25 and 50 μg/ml of PAR, respectively.For the Q analysis method, the absorbances of mixed sample solutions were measured at 243.5.0 and 277.0 nm, respectively.The concentration of each drug in sample was calculated using above mentioned equations given in Q analysis method.
For the fi rst order derivative method, the absorbances of mixed sample solutions were measured at 243.5 and 303.5 nm.The concentrations of DRO and PAR present in the sample solution were determined against calibration curve in quantitation mode.The tablet analysis results obtained by proposed methods were validated by statistical evaluation [10,11] (Table 1).
To study accuracy and reproducibility of proposed methods, recovery studies were carried out at 80, 100 and 120% level of label claim.Inter-day and intra-day precision of the assay was determined by analyzing the drug sample at two different concentrations and % RSD were found to be less than 0.14 and 0.16 for DRO and PAR, respectively.LOD values were found to be 0.12 μg/ml, 0.15 μg/ml of DRO and 0.18 μg/ml, 0.20 μg/ml of PRA for method I and II, respectively.LOQ values were found to be 0.32 μg/ml, 0.40 μg/ ml of DRO and 0.51 μg/ml, 0.58 μg/ml of PAR for method I and II, respectively.
The proposed Q analysis method is also a simple and easy method.This method requires determination of absorbances of sample solution at the two selected wavelengths and few simple calculations.The first derivative spectrophotometric method requires spectral data processing and hence can be applied only on recording spectrophotometers with such facilities.This method was employed totally to eliminate the spectral interference from one of two drugs while eliminating the other drug.This was achieved by selecting the zero crossing point on the derivative spectra of one drug as the wavelength for estimation of other drug.First derivative method is simple, less time consuming, no manual calculation is required and gives better results.
Both the developed methods were found to be simple, rapid, accurate and precise for routine simultaneous estimation of both drugs in tablet dosage form.The value of standard deviation was satisfactorily low and the recovery was close to 100% indicating the reproducibility and accuracy of the methods.Key words: Amlodipine besilate, area under curve method, hydrochlorothiazide, losartan potassium, reverse phase high performance liquid chromatography, simultaneous equation method, area under curve method

Fig. 3 :
Fig. 3: First order derivative spectra of paracetamol Average of six estimations.DRO and PAR denotes drotaverine hydrochloride and paracetamol respectively.Method I and II denote Q analysis and fi rst order derivative method respectively.T1 and T2 are two different brands of tablet (T1-Dropar and T2-Drotin plus). *