Porous Carriers for Controlled/Modulated Drug Delivery

Considerable research efforts have been directed in recent years towards the development of porous carriers as controlled drug delivery matrices because of possessing several features such as stable uniform porous structure, high surface area, tunable pore size and well-defined surface properties. Owing to wide range of useful properties porous carriers have been used in pharmaceuticals for many purposes including development of floating drug delivery systems, sustained drug delivery systems. Various types of pores like open, closed, transport and blind pores in the porous solid allow them to adsorb drugs and release them in a more reproducible and predictable manner. Pharmaceutically exploited porous adsorbents includes, silica (mesoporous), ethylene vinyl acetate (macroporous), polypropylene foam powder (microporous), titanium dioxide (nanoporous). When porous polymeric drug delivery system is placed in contact with appropriate dissolution medium, release of drug to medium must be preceded by the drug dissolution in the water filled pores or from surface and by diffusion through the water filled channels. The porous carriers are used to improve the oral bioavailability of poorly water soluble drugs, to increase the dissolution of relatively insoluble powders and conversion of crystalline state to amorphous state.


November -December 2009
The goal of any drug delivery system is to provide the therapeutic amounts of drug to the proper site in the body to achieve promptly and to maintain the desired drug concentration [1] .Delivery of drugs by means of controlled release technology began in the 1970s and has continued to expand rapidly [2] .Various drug delivery systems, such as liposomes, micelles, emulsions, polymeric micro/nanoparticles have been showing great promise in controlled and targeted drug delivery.Among these systems porous materials are emerging as a new category of host/guest systems [3][4][5][6] .
Greater attention has been focused on the development of porous materials as controlled drug delivery matrices because of possessing several alternatives features such as stable uniform porous structure, high surface area, tunable pore sizes with narrow distribution and well defined surface properties [7,8] .Owing to wide range of useful properties, porous carriers have been used in pharmaceuticals for many purposes including development of novel drug delivery systems such as floating drug delivery system, sustained drug delivery system and improvement of solubility of poorly soluble drugs [9][10][11][12][13][14] .These materials possess vast amounts of nanopores that allow the inclusion of drugs [1] .These features allow them to adsorb drugs and release them in a more reproducible and predictable manner.The use of mesoporous, microporous and nanoporous carriers used for drug delivery is a part of growing research [15,16] .
Liquid penetration into and its subsequent flow through such porous materials depends on both the molecular and the bulk property of the liquid and the geometric and surface property of the porous medium.The required displacement depends upon the pore size, surface tension of liquid and the contact between surface and liquid [17] .
When a porous hydrophobic polymeric drug delivery system is placed in contact with the appropriate dissolution medium, release of drug to medium must be preceded by the drug dissolution in the water fi lled pores or from surface and by diffusion through the water filled channels [18] .Drug release from the porous carrier may be complete within 10 min or be incomplete after several hours or days.Solvent polarity and surface properties play an important role in the adsorption and release from the porous carriers [19,20] .
Adsorption is the interphase accumulation of concentration of substances at a surface or interface.Adsorption is the phenomenon by which the molecules of gas, vapour and liquid spontaneously concentrate at a contacting surface without undergoing chemical reaction, thereby forming a surface or interfacial layer.Molecules that adsorbed on the solid surfaces are referred as adsorbate and the surface to which they are adsorbed referred as substrate and adsorbent.

Advantages of adsorption for drug delivery:
Adsorption and entrapment of drug molecules in carrier granules leads to an enhancement of physicochemical stability of the drug.The presence of larger number of hydroxyl groups that form inter-and intra-molecular hydrogen bonded structure were identifi ed as a factor for enhancing dissolution.Adsorption is readily adoptable for thermolabile drugs and carriers.Improved dissolution rates are attributed to decreased drug particle size with a consequent increase in the surface area and to increase in the thermodynamic activity of drug in dispersed state.

Porosity:
The word pore comes from the Greek word 'ποροσ', which means passage.This indicates the role of a pore acting as a passage between the external and the internal surfaces of a solid, allowing material, to pass into, through or out of the solid.Fig. 1 represents the physical picture of porous solid and various types of pores that may occur in a solid.It is probable that pores are irregular in shape and may also be interconnected.An open pore is one which is connected to the external surface of a solid and allows the passage of an adsorbate through the solid in contrast to the closed pore that is a void within the solid which is not connected to the external surface and hence is isolated.Third type of pore is the transport pore that connect different parts of the external surface of the solid to the inner micro porosity and fi nally the blind pores that are connected to the transport pores but do not lead to any other pore or surface.Porosity is the collective term for these pores and their distribution in the structure of the solid.Based on the pore size the porosity is classified as microporosity, mesoporosity and macroporosity as given in Table 1.

Microporosity:
The micropores are formed as the result of imperfect stacking of constituent molecules and packing arrangements of the bulk material, producing a lack of crystallite alignment, and small pseudo-graphitic crystallites.Their shape has been shown, by TEM studies to be either slit-like or convoluted in shape.Adsorption into micropores is completely reversible.The class of micropores may be subdivided into three separate groups; ultramicropores, micropores and supermicropores.Ultramicroporosity (diameter < 0.5 nm) is usually responsible for activated diffusion and the pore diameter is comparable to that of the adsorbate molecule.Microporosity (diameter~0.5-1.4 nm) fills quickly, within the first few minutes of adsorption and overlap of the pore wall potentials is the pore filling mechanism for adsorption in micropores.Supermicroporosity (size-1.4-2.0 nm) promotes co-operative pore fi lling wherein monolayer formation occurs and the pore diameter is effectively reduced enhancing the adsorption potential of the pore  hence increasing adsorption and completing the pore fi lling at low relative pressure.
The micropores provide sites of maximum adsorption potential for an adsorbed molecule/atom and within the pore.Due to the close proximity of the walls of micropores an interaction of the polyionic potentials, the result of overlapping of the dispersion fi elds may occur resulting in a relatively deep potential energy well and enhanced adsorption at a given pressure.Hence, diffusion into the ultramicropores has a signifi cant activation energy associated with it.The pore fi lling process may be divided into three steps, first is the monolayer formation, second the pore fi lling by co-operative effects and third is completion of the pore fi lling process.

M esoporosity:
Mesopores are the result of major defects in the structure of a solid and serve as passages, providing a transport system, to the micropores.These are the pores which give rise to the phenomenon of capillary condensation.The mesopores fill by multilayer formation.The pore diameters, greater than 2 nm but less than 50 nm according to IUPAC defi nition, are so large that at low relative pressures monolayer coverage occurs followed by further layers and the adsorbed fi lm acts as a nucleus upon which capillary condensation may take place.

Macroporosity:
The micropores are considered important in the process of adsorption whereas the meso-and macropores primarily act as transport pores.Major lattice structure defects, such as racks, fi ssures and etching channels, within a solid lead to the formation of macropores which may be treated as an open surface.It is possible to observe macroporosity by optical microscope and scanning electron microscopy as they are of the order 50 nm and greater.There is no actual upper limit to the diameter of the pores but it is usually 1-2 mm [21] .

METHODS OF DRUG LOADING
Coating the drug-loaded core particles with a polymer fi lm is one of the most widely investigated technologies to make multi-particulate dosage forms.
A large variety of materials like Nonpareil ® sugar beads and Celphere ® crystalline cellulose, have been applied as core particles for this technology.However, the drug-loading ratio is relatively low, since the loading area is limited to the outer surface of core particles.To overcome these pharmaceutical difficulties, there has been increasing interest in the use of porous materials as the drug-loading core by making use of their high surface area [24] .Drug-loaded particles appear particularly suited for controlled release and drug targeting.These systems are expected to enhance the bioavailability of poorly absorbed drugs, entailing a lowering of the therapeutic dose.

Simple mixing:
In this method, adsorbent is placed in the drug solution and stirred for suitable time using magnetic stirrer.The solution is then allowed to stand for 1 h, separated and dried over 24 h at 60º.This method is used for the variety of drugs like ibuprofen, dexamethasone, griseofulvin, ranitidine and furosemide [14] .

Solvent evaporation:
Adsorbent is closely sieved in the range of 250-350 µm to nullify the effect due to variation in particle size.Drug was loaded in solvent followed by the constant addition of the adsorbent, kept to evaporate under ambient conditions [7] .

Loading under high pressure:
Drug were mixed with the adsorbent in suffi cient ratio and put into the high pressure adsorption equipment (fi g. 2) for a period over 24 h.After being washed with a deionised water to get a rid of unentrapped drug, the powder was dried in vaccum oven at 65º for 5 h.This method is used for the loading of Brilliant Blue [25] .

Vaccum process:
Adsorbent is placed in the drug solution and the mixer evacuated for suitable time after which the vaccum was released.The adsorbent and drug solution were then allowed to stand for 1 h.Following this, solids separated using filter paper and dried for 24 h at 60º.Various drugs like diltiazem hydrochloride, benzoic acid, sodium benzoate are used for loading on the adsorbent [14] .In another method, drug and adsorbent are mixed in the suitable volatile solvent for 6 h and evaporated the obtained mixer under reduced pressure.The obtained powder was dried in vaccum for 3 h.This method is used for the loading of hydrophobic drugs like phytonadione [26] .

Stirring in drug solution or suspension:
Adsorbent is stirred in drug solution or suspension followed by drying in simple tray drier.Instead of using excess medium requisite minimum drug solution or suspension were loaded.Therefore vaccum process is not essential and high yield can be achieved.This method can be applied to a variety of drugs like theophylline [24] .

Layer-by-layer adsorption (LbL):
The technique was usually performed in the aqueous solution at room temperature and thus it was suitable to encapsulate polypeptide and proteins drugs of poor stability.A LbL assembly of two oppositely charged polyelectrolytes at solid surfaces was developed as the alternating adsorption of these polyelectrolytes on a charged substrate due to their electrostatic attraction and the complex formation resulting in the defined macromolecular layer on the surfaces [1] .

Improvement in dissolution:
Mesoporous materials offer a potential means to increase the dissolution of poorly soluble drug via effects on surface area or crystallinity.At pore sizes only a few times larger than the drug molecule, the formation of crystalline material is restricted by the confi ned space of the pores, thus retaining the drug in its noncrystalline, amorphous form.The amorphous form is known to exhibit higher dissolution rates than the crystalline phase [27] .

Chemical and pharmaceutical purifi cation:
Adsorption in carbonaceous adsorbents is suitable for the decolourisation and purifi cation of a wide range of organic and inorganic compounds including: amines, hydrochloric and other mineral acids, amino acids, glycols, hydrocarbons.Catalysts can be poisoned or fouled by low concentration organic compounds, sulfur or mercury species.Carbonaceous materials, non impregnated and impregnated types, are typically used as "guard beds" to protect the catalysts (from fouling and/or deactivation) and the equipment (from corrosion) in streams such as natural gas, acetylene, ethanepropane and ethylene oxide.

Food purifi cation and decolorization:
Adsorption in carbonaceous adsorbents is widely used for the decolourisation of natural and synthetic sweeteners, decolorization of cane sugar syrup, decolorization of vitamines and purification of glycerin.

Improvement in bioavailability:
Low density porous carriers such as porous silicon dioxide (Sylysia), polypropylene foam powder (Accurel), porous calcium silicate (Florite), magnesium aluminometa silicate (Neusilin), and porous ceramic with open or closed pore structure that provide large surface area are used for the improvement of dissolution and bioavailability of poorly soluble drugs like meloxicam, aspirin, indomethcin [28] .

Solubility improvement:
Florite RE (FLR) is a porous calcium silicate that possesses many interparticle and intraparticle pores, particularly of sizes 12 and 0.15 μm, respectively, on its surface.FLR is easily dispersible in all aqueous fluids and has been used to adsorb oily and other drugs, as a compressive agent in pharmaceuticals, and to improve solubility [9] .

Improvement of surface affi nity:
Otsuka et al. [26] investigated the surface-modifi cation of silica gel with the silane coupling to improve the surface affinity to an oily medicine, phytonadione.However, a rapid release during the whole process, especially initial burst release has been observed in some cases when inorganic porous particles were used as the drug host.

Sustained/controlled release:
Several porous minerals have been used including synthetic zeolite, silica xerogel materials, porous hollow silica nanoparticle, porous hydroxyapatite, porous silica-calcium phosphate composite, porous calcium carbonate microparticle and other porous ceramics.These materials possess vast amounts of nanopores that allow the inclusion of drugs in them and useful in making sustained/controlled release formulations [29][30][31][32][33][34] .Accurel MP 1000 is characterized by open porous network with pore size predominantly in the micro and mesoporous range.This microporous adsorbent has been evaluated for the development of floating drug delivery system and stem development [35] .A single unit fl oating drug delivery system consisting of low density microparticles were developed using porous carrier material (foam powder), drug and polymer, to increase the gastric residence time of drug delivery systems [36][37][38][39] .The fl oating behaviour of the low density drug delivery system could successfully be combined with accurate control of drug release.
Ohta et al. [24] investigated the use of Silica gel as core particles to design a simple preparation for controlled delivery system with high drug content.Drug loading was carried out by immersing the silica gel in a pre-heated drug solution or suspension.The drug-loaded silica gel was coated with hydroxypropylmethylcellulose (HPMC) and an aqueous dispersion of ethylcellulose (Aquacoat ® ) to control the drug release.Layer by Layer (LbL) selfassembly technique has been a powerful tool where polyelectrolyte multilayer films were elaborated on various particles through alternating deposition of oppositely charged polyelectrolytes mainly due to the electrostatic attraction.LbL adsorption on the porous carriers showed potential applications in controlled release, cosmetic for the delivery of the proteins like bovine serum albumin, glucose oxidase, urease and superoxide dismutase), as the depositing species to prepare bioactive core-shell particles.Ito et al. [28] developed the self emulsifying drug delivery system by using three kinds of adsorbents microporous calcium silicate (Florite™ RE), magnesium aluminometa silicate (Neusilin™ US 2 ) and silicon dioxide (Sylysia™ 320) with low molecular weight heparin (LMWH).The results suggest that adsorbent system is useful as an oral solid delivery system of poorly absorbable drugs such as LMWH.Table 2 compiles the work done on drug release modulation by use of porous carriers.

Others applications:
Porous ceramics are bodies comprised of a threedimensional array of hollow polygons, known as cells.If the individual cells are interconnected the porous ceramic is termed open-cell, while if they are isolated from each other, the porous ceramic is termed closed-cell.They can also be partly open or partly closed.These properties have found many applications including catalyst supports, filters for molten metals and hot gases, refractory linings for furnaces, and porous implants in the area of biomaterials [40] .Crystalline microporous materials generally have a narrow pore size distribution.This makes it possible for a microporous material to selectively allow some molecules to enter its pores and reject some other molecules that are either too large or have a shape that does not match with the shape of the pore.A number of applications involving microporous materials utilize such size and shape selectivity.

CONCLUSIONS
Today the porous carriers have a major role to play in the pharmaceutical industry.Their inner structure consists of unidirectional channel like pores that forms a hexagonal pattern.The presence of porous structure like microporous, mesoporous and macroporous structure was found to be essential in providing the sustained drug delivery systems, fl oating drug delivery systems and improvement of poorly Alendronate [41] Hydroxyapatite (HA) Simple Mixing Alendronate modifi ed nanoparticles had a strong and specifi c adsorption to HA.The amount of nanoparticle adsorbed on to HA tend to be smaller when the content of alendronate was decreased and the large block length of monomethoxy PEG was found to reduce the potency of alendronate.

Simple Mixing
The in vitro release study shows that surface modifi cation greatly decreased the Ibuprofen release rate i.e. 63% in 6 h and the release was complete in 1 h from pure silica SBA-15.The surface modifi ed with MgO created affi nity with acidic Ibuprofen molecules and retarded the release rate from the mesoporous matrix.

Solvent Evaporation
The drug release was investigated in phosphate buffer pH 7.2.All batches shows excellent in vitro fl oating property.Effect of solvent properties shows a positive infl uence on drug adsorption and release.Release profi le of some batches could be considered as gastro retentive drug delivery system.
Simple mixing New oral solid dosage form of low molecular weight heparin has been prepared by using surfactant and adsorbents.This study has been done to improve the intestinal absorption of low molecular weight Heparin.Results shows that adsorbent system is used as an oral solid delivery system of poorly absorbable drugs such as low molecular weight Heparin.
Loratadine [44] Porous Polystyrene Beads (PPB) Solvent Evaporation Porous Polystyrene Beads are potential carrier for solidifi cation of SES, with high SES to PPB ratios required to obtain solid form.Geometrical features such as bead size and pore architecture of PPB was found to govern the loading effi ciency and in vitro release from SES loaded PPB.

Layer-by-Layer adsorption
This method can offer high drug loading capacity and high encapsulation effi ciency.Also the release behaviour can be controlled since the cell thickness and its permeability, was readily adjusted on the nanometer scale by polyelectrolyte adsorption cycle.
The Insulin loaded nanoparticles appears to be especially promising for the parenteral administration on insulin in diabetic patients.

Microporous Bioactive Glass (MBG) Immersion
The amount of drug loading was greatly infl uenced by the well ordered mesoporous structure and the amount of drug loading of MBG was three times more than that of conventional 58S.So the well ordered mesoporous bioactive glasses might be used as a bioactive drug release system for preparation of bone materials.
Ibuprofen [1] Porous Calcium Carbonate Vacuum Loading In this work, polymer/inorganic hybrid core-shell microcapsules were fabricated for controlled release of poorly water-soluble drugs.The porous inorganic particles are useful to load drugs in amorphous state and the polyelectrolyte multilayer fi lms coated on the particle assuage the initial burst release.November -December 2009 Dexamethasone [22] Nanoporous Titanium Dioxide (TiO 2 )

Immersion
This novel drug-loading scheme on a biocompatible surface, will benefi t patients who require the deployment of drug-eluting implants.Anticoagulants, analgesics and antibiotics for drug delivery during the time of maximal pain or risk for patients undergoing orthopaedic procedures.Theophylline [24] Silica Gel Immersion in drug solution and suspension Evaluation of the drug-loading process indicates that drug deposition in the pores occurs during the loading process and the drug-loading effi cacy is strongly related to the drug solubility.An HPMC undercoating effectively suppresses the drug release, as it smoothes the drug-loaded core surface and aids in the formation of a continuous Aquacoat ® coating fi lm.
Antipyrine, Ibuprofen, Griseofulvin, Ranitidine and Furosemide [27] Mesoporous Silicon Simple Mixing When the dissolution rate of the free/unloaded drug was high, the microparticles caused a delayed release.However, with poorly dissolving drugs, the loading into the mesoporous microparticles clearly improved dissolution.In addition, pH dependency of the dissolution was reduced when the drug substance was loaded into the microparticles.

Simple Mixing and Solvent Evaporation
For their surface areas and ordered mesoporous structure, micelle templated silica materials constitute a potentially interesting drug carrier for non-water soluble drug carrier.The association of nano-structured mineral to the molecular state of the drug presents a great interest for pharmaceutical application as it allows a control of the kinetic delivery of the lipophillic drugs.
Fenofi brate [47] Poly (ethylene glycol)-block-poly (€-caprolactone) Direct Dissolution, Solvent Evaporation and Dialysis PEG-b-PCL in acetonitrile assembles with fenofi brate in to drug loaded polymeric micelles with the addition of water and the subsequent removal of negative ACN-Water azeotrope.
Haemoglobin [48] Mesoporous Ceramics Adsorption This study demonstrates that the haemoglobin adsorbed aquasomes can carry the oxygen satisfactorily and it also establishes the superiority of haemoglobin aquasomal formulation as artifi cial blood substitute.
Brilliant Blue (BB) [2] Porous Hollow Silica Loading under high pressure After being loaded into the inner core and on the surfaces of the nanoparticles, BB was released slowly into a bulk solution for about 1140 min as compared to only 10 min for the normal SiO 2 nanoparticles, thus exhibited a typical sustained release pattern without any burst effect.It showed that PHSN have a promising future in controlled drug delivery applications.

Hot melt Granulation
A competitive balance between hydrogen bonding of the drugs with Neusilin and Ostwald ripening determines drug dissolution from solid-dispersion granules upon storage.
Verapamil Hydrochloride [12] Polypropylene Solvent Evaporation The size of microparticles was almost independent of the amount of the drug loading, but strongly depends on the amount of polymer.The drug was partly dissolved and partly in the amorphous form distributed throughout the system.Benzoic Acid, sodium Benzoate, Diltiazem [14] Porous Ceramics-N-light N 3 , Starlight SLK 1000 and Carbolite 16/20

Vacuum Loading
The drug loading was infl uenced by the solution concentration and by the porosity and bulk density of ceramic.In vitro dissolution testing of the loaded porous microparticles showed an initial burst release of each drug followed by sustained releases.The release was infl uenced by the surface.
water soluble drugs etc.Therefore, in the years to come, there is going to be continued interest in the porous carriers to have better materials for drug delivery systems.
Sodium Ampicillin [50] Hydroxyapatite (HA) and Glass reinforced hydroxyapatite (GR-HA) -The release kinetics studies in which where a large amount of sodium ampicillin was released, followed by slower release rate and then a fi nal stage where the release amount approaches zero have indicated that the possibility of using these materials as possible carriers for drug delivery.

Simple Mixing
The gelatine coated PLGA microspheres has higher interaction with fi bronectin compared with the other gelatine surface modifi ed PLGA microspheres.Dexamethasone was released slowly from gelatine surface modifi ed PLGA microspheres.
Phytonadione (VK1) [26] Silica Gel Solvent Evaporation and Vacuum loading The VK1 release from the modifi ed silica gels was initially rapid, slowed markedly after 1 h, and continued for more than 24 h.The amount of VK1 released from the modifi ed surface silica gels by C7, C18 or F3 increased with increasing density of the surface modifi cation group.The mean drug release moment (MDT) decreased with an increase in surface-modifi ed group density.
Vasopressin [52] Accurel® (polypropylene) -The present module is a novelty among the known membrane controlled drug delivery reservoir systems, since it has been loaded by an unsaturated drug solution and because the encapsulating membrane is not the sole release rate controlling factor.
Potassium Chloride [18] Porous Ethyl Cellulose Solvent Evaporation Release of water soluble drug from polymeric system of known void fraction may be explained by the considering a dissolution-controlled regime.

Fig. 1 :
Fig. 1: Magnifi ed physical picture of a porous solid particle Physical picture of porous solid showing A-surface roughness, Bink bottle (blind pores), C-closed pores, D-transport pores (pores through the solid) and E-cylindrical blind

Fig. 2 :
Fig. 2: Diagrammatic representation of the drug loading in porous adsorbent under high pressure [1] Drug loading under high pressure: (i) adsorbent (air is adsorbed in the adsorbent); (ii) drug gets loaded into the inner core of the adsorbent under high pressure; (iii) the drug loaded adsorbent.