A cytogenetic study of couples with recurrent spontaneous abortions and infertile patients with recurrent IVF / ICSI failure

It is demonstrated by the high frequency of abnormalities found in sample fetal or embryonic tissue.[1] When a woman has had two or more miscarriages, she will be under the care of gynecologist to seek professional help in the hope that a cause and care will be found. [2] In the other way, the development of IVF and ICSI and related techniques has increased the possibility of obtaining babies from infertile patients. Today recurrent implantation failure is the major reason for women completing several IVF/ICSI attempts without having achieved a child, and is probably also the explanation for many cases of unexplained infertility. Most causes of recurrent miscarriage are still poorly elucidated, but from a theoretical point of view recurrent implantation failure and recurrent miscarriage are suggested to have partly overlapping causes.[3] It is well known that lower implantation rate and higher spontaneous abortions rate are closely related with the chromosomal abnormalities of both parents. Even in some patients, unexplained multiple IVF/ICSI failure has been frequently reported.[4] An association between human infertility and chromosomal abnormalities has been known for long time;[5] thus, it would not be unusual to Þ nd chromosomal abnormalities in couples attending an infertility clinic.


Introduction
Reproduction in human being is genetically risky process and terribly incompetent.The most common outcome of conception is embryonic or fetal death.Near one-third of conceptions do not result in the delivery of a baby.Miscarriages are clinically detectable pregnancies that fail to progress.They are common and often remain unexplained, although it has shown that a major cause of this demise is attributed to chromosomal abnormalities.result, karyotyping of White Blood Cells of every person attending the infertility clinic would be necessary to identify those with genetic defects.The aim of this study was to examine two groups of couples from infertile marriage, in an attempt to identify any clinical abnormality that could be of predictive value for chromosomal aberrations.

Materials and Methods
The patient population consisted of 221 Iranian individuals (102 men and 119 women) who attended the infertility clinic with a history of repeated spontaneous abortion and IVF/ICSI failure.We divided our samples in two groups: Þ rst group consisted of couples with recurrent spontaneous abortion (79 individuals) and the second group was those who had at least three IVF or ICSI failures (142 individuals).On epidemiological evidence, the deÞ nition of recurrent miscarriage should be three or more consecutive pregnancy losses.Women meeting the deÞ nition can be subdivided into primary and secondary groups, respectively, consisting of those who have lost all previous pregnancies and those who have had one successful pregnancy followed by consecutive losses. [7]n the present study, the patients had a history of three or more abortions and did not have any children.Their managements were started with clinical examination by gynecologist and urologist and then by a genetic counselor.The anatomical problems were ruled out by gynecologist and urologists.In all women antibodies against toxoplasmosis, rubella and cytomegalovirus (CMV) were analyzed by enzyme-linked immunosorbent assay (ELISA) Trinitin 99% kits.The blood samples of both male and female partners were subjected to a white cell chromosome analysis.After genetic counseling, family pedigree was drawn by genetic counselor.Karyotyping was conducted by analysis of G-banded chromosomes using 5 mL heparinized peripheral blood sample.
Metaphase spreads were made from phytohemaglutinin stimulated peripheral lymphocytes using standard cytogenetic techniques.Cultures were harvested and Karyotyping was performed on G-bands produced with trypsin and Giemsa (GTG)-banded chromosome preparations.The chromosomal status was analyzed using CytoVision Ultra ver.4.0 from Applied Imaging (New Castle, UK).At least 25 metaphases were analyzed for each patient.If there was any sign of mosaicism, 50 metaphases from two independent cell cultures were analyzed.All chromosomal abnormalities were reported in accordance with the current international standard nomenclature. [8]The χ 2 -test and one way analysis of variance (ANOVA) were used for statistical evaluation by SPSS software (version 11.5).The level of P < 0.05 was considered as signiÞ cance.

Results
The median age of male partners was 35.36 ± 0.85 and the median age of female partners was 31.0 ± 1.1.
There did not appear to be an age-related distribution of gonosomal aberrations.The results obtained are summarized in Tables 1-3 and shown in Figures 1 and 2.
Abnormal karyotypes were found in 21 (9.50%) of the 221 subjects studied.We were not aware of any data available on the rate of anomalies in an equivalent fertile population.Of these 21 subjects, 4 (19.04%)exhibited sex chromosomal abnormalities.The remaining 17  1 and 2].
Inversion of chromosome 9, normally not considered as a pathological chromosome alteration, was also included in Table 1 for the sake of completeness.The frequency of aberrations was 5.88% in the male partners.
Numerical or structural abnormalities were documented in 3.61% of the female partners.There was signiÞ cant difference for the rate of aberrations between male and female partners (P < 0.05) [Figure 2].
The results also were analyzed according to the group of patients and are shown in Table 3 and Figures 1 and   2. The rate of anomalies was higher in both male and female with recurrent abortions history than those who had IVF/ICSI failure (P < 0.05).Statistical analysis of data showed a statistically signiÞ cant difference for the rate of abnormalities between the two groups under investigation.

Discussion
1] The genetic factors represent more than 50% of early gestation spontaneous abortion and associated with fetal chromosomal abnormalities. [12]The genetic etiology  for multiple spontaneous pregnancy loss includes an unbalanced chromosome rearrangement, which may be the result of one parent being a carrier for a balanced chromosome rearrangement. [13]In 4-8% of couples with recurrent pregnancy loss, at least one of the partners has chromosomal abnormality that probably contains balance chromosomal abnormalities. [2]The results of the present study showed 13.92% chromosomal abnormalities in these individuals [Figure 1].The prevalence of chromosomal aberrations among couples with repeated spontaneous abortions varied in different studies, from none [14] to as high as 21.4%. [15]In the present study, incidence of chromosomal abnormalities among studied couples with repeated abortions was 13.92% which is higher than chromosomal aberrations prevalence among couples with repeated abortions found by Pescia et al. (5%), [16] Palanduz et al. (6.5%), [17] Al Hussain et al. (7.7%), [18] and Mohammed (7.8%), [19] but close to that reported by Butler and Hamill (17.8%). [20]On the other hand, the types of abnormalities can play an important role in the effect of aberration.Fryns and Van Buggenhout reported that of the chromosome abnormalities observed in couples with repeated abortions, two-thirds were balanced autosomal translocations. [21]The rate of these abnormalities in this research was less than their report and was 14.28% of all the aberrations.X-chromosome mosaicism, which was seen in our karyotypes, is usually associated with abnormal development and reproductive performance, including recurrent spontaneous abortion. [22]We demonstrated X-chromosome mosaicism in two females (1.68%) out of 119 women experiencing recurrent abortion and IVF/ICSI failure.However, the reproductive performance of X-chromosome mosaicism is highly variable and difÞ cult to deÞ ne. [23]The incidence of karyotype abnormalities among infertile men has been reported to range between 2.2% and 19.6% [24][25][26] and numerical or structural abnormalities have been documented in near 10% of the female partners [27] similar to our observations shown in Figure 2.Moreover, other studies showed that structural chromosomal abnormality is the most common chromosomal abnormality in couples with recurrent abortions especially couples undergoes ART. [28,29]In vitro fertilization plus preimplantation genetic diagnosis (PGD) is an important step in the management of these couples. [10]One in 500 people carries a balanced translocation.When one member of a couple carries a balanced chromosome translocation, the risk of having a miscarriage is approximately doubled.In 3-5% of couples with recurrent miscarriage, one partner has a balanced translocation. [2]According to some researches, early on, genetically normal and abnormal embryos have similar appearance.On the basis of morphology alone, the genetic integrity cannot be determined; therefore, transferring of embryo in IVF and ICSI is some how risky and nonselective, so the risk of transferring the abnormal embryo is still persist.It can be resulted in IVF or ICSI fail. [30,31]The commonly reported human inversion was inversion (9) (p12;q13).The role of pericentric inversion of chromosome 9 in infertility and pregnancy losses is still very controversial. [32]Although inversion (9) has been associated with repeated spontaneous abortions in several families, studies on unselected series of couples have usually failed to demonstrate a relationship between inversion (9) and repeated abortions. [33,34]This study has shown that the incidence and distribution of total chromosomal abnormalities among our patients are comparable to that reported worldwide.In the future to complete this study, cytogenetic analysis of the abort uses should be done, which help the family in other pregnancies.In other research has indeed documented that both syndromes can be caused by the same embryonic chromosomal abnormalities and the same maternal endocrine, thrombophilic, and immunological disturbances.Consequently, many treatments attempting to normalize these abnormalities have been tested or are currently used in women with both recurrent implantation failure and recurrent miscarriage.However, no treatment for the two syndromes is at the moment sufÞ ciently documented to justify its routine use. [3]We concluded from all the previous results that cytogenetic studies should be performed to all couples with two or more

Figure 1 :Figure 2 :
Figure 1: The abnormality rate in different study groups spontaneous abortions and also in patients with recurrent IVF/ICSI failure.In a case of detected chromosomal aberration; the patient should be counseled individually according to the type of anomaly.This study should help physicians working in the region to realize the contribution of chromosomal abnormalities to cases of repeated fetal loss.It should also help in setting priorities of cytogenetic screening in individual cases.With subsequent possibility of the performance of PGD using the routine in vitro fertilization, biopsy of the embryos allows the selected transfer of chromosomally balanced embryos.The translocation PGD has been applied successfully in several centers and should be now considered as a realistic treatment option for translocation carrier.