Cases Journal BioMed Central

A 65-year-old man had suffered from rectal bleeding during defecation for a few weeks, admitted to our department. Laboratory findings were normal except a slight elevation in the level of alkaline phosphatase. Multiple polypoid lesions were observed in colonoscopic examination. The histological and immunochemical evaluation showed atypical lymphoid cell proliferation and lymphoepithelial lesions on the colonic mucosa, staining with CD20 (CD20 × 100). After the diagnosis had been confirmed as low grade mucosa associated lymphoid tissue lymphoma. Abdominal computed tomography revealed polypoid lesions throughout the colon and multiple milimetrics lymphadenopathies in the mesentery. The patient was treated with a chemotherapy regimen. During the follow-up, colonoscopic examination and blind biopsies were repeated in every 6 months, revealed endoscopically and pathologically normal mucosa each time. The patient is still alive without any recurrence of the disease 36 months after the diagnosis.


Introduction
Since the first successful hand transplantation in Lyon in 1998, 38 hands (18 unilateral and 10 bilateral) and two digits have been transplanted in 30 individuals in different countries [1]. Notwhistanding the value of such experience pioneering composite tissue transplantation and allowing to explore other transplant frontiers such as face and limb transplantation, clinicians continue to debate about the risk acceptability of such procedures [2].
Indeed, the functional and psychologic benefits of hand transplantation may be counterbalanced by the risk of potentially severe systemic complications due to immune suppression. In some instance, the clinicians may face the difficult decision of amputating a successfully transplanted hand in order to interrupt the immunosuppressive treatment in the attempt to solve a life threatening opportunistic infection. One of the most challenging viral infections in transplant recipients is human cytomegalo-virus (HCMV) infection, namely in HCMV-seronegative recipients (R -) of grafts from HCMV-seropositive donors (D + ) [3].
The case of a D + Rhand transplant recipient with recurrent episodes of disseminated HCMV infection associated with impaired HCMV-specific CD4 + and CD8 + T cell response is described.

Case presentation
In October 2002, a 33 year-old HCMV-seronegative caucasic male who had lost his dominant right hand at the wrist level in a car crash 10 years before was submitted to hand transplantation from a HCMV-seropositve donor. Immune suppressive treatment included tacrolimus, mycophenolate mofetil and steroids. Kinetics of HCMV infection was monitored by quantitative determination of antigenemia and DNAemia [4]. Antigenemia consists of quantitative determination of HCMV pp65-positive/2 × 10 5 leukocytes examined in a cytospin preparation [4]. DNAemia consists of quantitation of HCMV genome by PCR in blood and was expressed as HCMV DNA copy number/10 uL whole blood [4].
HCMV antiviral prophylaxis with i.v. ganciclovir (5 mg/ Kg/die) was administered for 30 days starting fom day 2 after transplantation. Afterwards, a preemptive antiviral treatment approach was adopted. In detail, antiviral drug were administered in the absence of overt clinical symptoms when in the presence of active HCMV infection as documented by positive antigenemia values [4].
On day 42, a severe acute rejection episode required antithymocyte globulin administration to be controlled. Soon thereafter, HCMV antigenemia level started increasing and by day 85 reached 1200 pp65-positive/2 × 10 5 leukocytes examined, in the absence of overt HCMV disease. Administration of foscarnet, cidofovir and anti-CMV hyperimmune globulin was successful in controlling HCMV infection. Preemptive treatment with valgancyclovir was then scheduled to initiate at the first confirmed positive antigenemia result in order to prevent HCMV disease. During the following 3 years, antigenemia and DNAemia were found to be repeatedly positive ( Figure  1A), thus prompting administration of several courses of antiviral treatment. The prolonged and repeated episodes of HCMV disseminated infection in the absence of other opportunistic infections suggested a potential selective impairment of HCMV-specific T-cell mediated immune response. The hypothesis was verified by investigating HCMV-specific CD4 + and CD8 + T-cell responses by a newly developed assay [5]. In detail, after in vitro differentiation from peripheral blood mononuclear cells (PBMC) separated by Lymphoprep™ (Axis-Shield PoC AS, Oslo, Norway) density gradient centrifugation, immature den-dritic cells (DC) were infected for 24 h with an endotheliotropic strain of HCMV. As a control, DC were mockinfected with the clarified medium of uninfected endothelial cell cultures. HCMV-infected or mock-infected DC were cocultured overnight with thawed autologous PBMC or the non-adhering fraction of PBMC in the presence of brefeldin A (Sigma, St. Louis, MO). Then, PBMC were tested for intracellular interferon-γ(IFNγ) production by a cytokine flow cytometry assay. The frequency of CD4 + and CD8 bright T cells producing IFNγ in response to HCMV stimuli were calculated by subtracting the value of the sample incubated with mock-infected culture medium or control antigen (consistently < 0.05%) from the test value. Absolute CD3 + CD4 + and CD3 + CD8 + T cell counts were determined on heparin-treated peripheral blood samples by a direct immunofluorescent flow cytometry method (Epics-XL, Beckman Coulter Inc.). To determine the total number of HCMV-specific CD4 + and CD8 + T cells, the percentages of HCMV-specific T cells positive for IFNγ were multiplied by the relevant absolute CD4 + and CD8 + T cell count. A cut-off value of 0.4 IFNγ-positive CD4 + and CD8 + T cells/μl indicated presence of HCMVspecific CD4 + and CD8 + T-cell immunity [6].
Due to unavailability of other D + Rcomposite tissue recipients as controls, results of T-cell immunity were compared with those of some D + Rsolid organ transplant recipients (SOTR) with primary HCMV infection. Immune suppressive regimen included cyclosporine-A, mycophenolate mofetil and steroids in 1 patient, tacrolimus, mycophenolate mofetil and steroids in 2 other patients, and cyclosporina-A, azathioprine, and steroids in the remaining 2 patients.
The hand transplanted patient showed absolute CD4 + T cell counts consistently < 100 for over 3 years after transplantation. HCMV-specific CD8 + T cell response was below the cut-off value till 21 months after transplantation, and HCMV-specific CD4 + T cell response was impaired till 35 months after transplantation ( Figure 1B). Coincidental with the lack of HCMV-specific CD4 + and CD8 + T cell response was the consistent positivity of viral markers in blood prompting antiviral treatment, while recovery of HCMV-specific CD4 + and CD8 + T-cell reponses was associated with spontaneous resolution of viral infection ( Figure 1A and 1B). To date, antigenemia and DNAemia remain negative in the absence of antiviral treatment. As shown in Figure 1C, control D + R -SOTR patients developing an effective T-cell response showed HCMV-specific CD8 + and CD4 + T-cell levels above the cutoff value by month 3 and 4 after transplantation, respectively. In parallel with the progressive increase of the HCMV-specific T-cell response, the progressive decrease of peak HCMV DNA levels in blood was observed. As a consequence, the number of HCMV infection episodes D + Rserostatus represents a major risk factor for severe HCMV infections in SOTR [3]. In addition, only D + Rpatients have repeatedly been reported to be at risk for development of drug-resistant HCMV infections among SOTR [4,7]. However, in the great majority of D + R -SOTR, HCMV primary infection can be successfully controlled by antiviral treatment. In addition, immunosuppressive regimens do not avoid in the majority of SOTR development of HCMV-specific T-cell response of the same amplitude as in immunocompetent individuals [8]. On the other hand, a few reports associated the emergence of drugresistant HCMV strains in SOTR following prolonged antiviral drug administration with the sustained impairment of HCMV-specific T-cell response [4,9,10]. Thus, the lack of HCMV-specific immunity in individual SOTR appears to play a major role in the pathogenesis of severe HCMV infections in transplanted patients.
Hand transplantation is not a life-saving procedure and it could be argued that it should be avoided in seronegative recipients since development of HCMV-specific immune response might be impaired in individual patients undergoing primary HCMV infection. In these cases, the antiviral treatment alone would be expected to be unable to control the repeated episodes of active HCMV infection with the potential risk of major complications such as development of overt disease or emergence of drug-resistant HCMV strains. For 3 years our patient was lacking HCMV-specific T cell immunity. This delay in mounting an efficient anti-HCMV cellular response is likely to be the major responsible for the recurrent episodes of disseminated HCMV infection. However, major complications were avoided in our patient by adoption of a tight virologic monitoring and repeated preemptive treatment courses. As a conclusion, a careful monitoring of HCMVspecific T-cell mediated immune response should parallel monitoring of HCMV load also in composite tissue transplant recipient.