Hepatoprotective Activity of Ficus carica Leaf Extract on Rifampicin-Induced Hepatic Damage in Rats

The petroleum ether (60-80°) extract of the above crude drug was prepared by Soxhlet extraction. The extract was tested for hepatoprotecive activity in rats. Four groups (1-IV) of six rats each, of either sex weighing between 150-200 g, were selected7. Group-I was normal control, fed with vehicle for 60 days. The animals in groups II and IV were orally administered with rifampicin 50 mg/kg for 60 days8,9. Animals in group III were administered orally the extract of Ficus carica leaf (200 mg/100 g) for 10 days. To animals in the group-IV petroleum ether extract of Ficus carica was given for after 61 days for 10 days. On the 71st day, pentobarbitione sleeping time was determined after which animals were weighed and sacrified. The liver was examined morphologically, dissected out, weighed and stored in 10% formalin. Blood was collected by cardiac puncture, serum separated Free Radic Res Commun 1989;7:121-8. Okhawa H, Ohishi N, Yagi K. Assay of lipid peroxide in animal tissue 12. by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8. Kinter M. Free radicals: A practical approach. In: Punchard, NA, Kelly 13. GJ, editors. Oxford: Oxford University Press; 1996. p. 136. Accepted 12 June 2008 Revised 14 November 2007 Received 4 November 2006 Indian J. Pharm. Sci., 2008, 70 (3): 362-364

Liver diseases remain one of the serious health problems.In the absence of reliable liver protective drugs in allopathic medical practices.Herbs play important role in the management of various liver disorders 1 .However, in ayurveda many indigenous plants have been used as hepatoprotective agents.A number of reviews are published stating the importance of plant drugs in the diseases of liver.Indigenous plant Ficus carica was selected for investigating hepatoprotective activity 2,3 .

Ficus carica Linn. (Moraceae), commonly known as
Anjir.The plant is considered to be a native of carica in Asia Minor and is grown in nearly all tropical and sub-tropical countries.In India its commercial production is limited to a few centers near Pune 4,5 .During casual conversation with tribal people of Maharashtra, it was found that they chewed the leaves of Ficus carica to treat Jaundice.However, no scientiÞ c work has been carried out on the leaves of Ficus carica to prove the hepatoprotective activity.Liver is a versatile organ in the body concerned with regulation of internal chemical environment.Therefore, damage to the liver inß icted by hepatotoxic agents is of grave consequence.There is an ever increasing need for an agent which could protect liver from damage, especially of one which facilitates regeneration by proliferation of parenchymal cells after damage arrest growth of Þ brous tissue.As such liver is highly affected primarily by toxic agents such as ccl 4 , paracetamol, D-galactosamine, alcohol, rifampicin and thioacetamide through different mechanisms.
In the present study, rifampicin is used as a hepatotoxic agent.Rifampicin is a major drug used for treatment of tuberculosis, but its chronic use is known to cause hepatotoxicity.The mechanism of hepatotoxicity induced by rifampicin is that it competes with bilirubin for transport across the liver cell and conjugated or unconjugated hyperbilirubinaemia can often occur in chronic hepatitis induced by rifampicin.
It was observed from the morphological examination of liver that treatment with rifampicin resulted in liver enlargement, which turned pale brown in colour.The group treated with petroleum ether extract of Ficus carica had livers which were similar to that found in the normal rats and were healthy in appearance.
Pentobarbitione sleeping time prolonged in rifampicin treated group.In spite of a large biological variation in sleep time, it was observed that the sleep time was reduced in the treated group.In the group of animals administered with petroleum ether extract the sleeping time was decreased as compared to rifampicin treated group and almost restored back to the initial sleeping time.For the study of liver weight and biochemical parameters, all the values obtained were subjected to statistical analysis.A signiÞ cant reduction in liver weight was observed in group treated with petroleum ether extract of Ficus carica.
The result of the biochemical tests revealed the elevation of serum enzyme level in rifampicin treated group compared to control group, indicating that rifampicin induced liver damage.A significant reduction was observed in SGPT, SGOT levels in the group treated with extract of Ficus carica.
The histological study showed recovery of the damaged liver cells in the drug treated group.The ruptured cells of intoxicated liver were reformed.The cytoarchitecure was restored to the same as normal liver.The protective effect appears to be maximum with extract of Ficus carica.Hence it is concluded that the petroleum ether extract of Ficus carica deÞ nitely possess liver protective activity.However, the data obtained in the present study appears to support traditional use of the medicinal plant in the treatment of liver diseases.
Rabeprazole and itopride hydrochloride 1 are available in combined capsule dosage form for the management of gastro esophageal reß ux disease (GERD).Literature survey reveals that various analytical methods [2][3][4][5][6][7][8][9] have been reported for rabeprazole and itopride hydrochloride in single dosage forms.The present paper aims to report a simple HPTLC method for estimation of rabeprazole and itopride hydrochloride in their combined dosage form.

TABLE 1 : EFFECT OF PETROLEUM ETHER EXTRACT OF FICUS CARICA LEAF ON RIFAMPICIN-INDUCED HEPATOTOXICITY Groups Pentobarbitone Liver weight Serum bilirubin SGPT SGOT (units/l) sleeping time (g) (mg%) (units/l) (min)
out and used for estimation of SGOT, SGPT by the Reitman and Frankel method, bilirubin by the Malloy and Evelyn method 10 .The sleeping time was assessed by injecting pentobarbitione sodium (30 mg/kg, ip) in water for injection 11 .The sleeping time, weight of liver and biochemical parameters are all presented in Table1.All animal experimental protocols have been approved by the Animal Ethics Committee (CPCSEA Reg.No. is 40/1999/CPCSEA.
All values are mean±SEM, n=6, P values: P<0.05, when compared to group-II* vs group-IV** and group-II* vs group-I #