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The discovery of a key prenyltransferase gene assisted by a chromosome-level Epimedium pubescens genome.

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Affiliations

  • 1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicines, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
      Authors
    • Shen G 1
    • Luo Y 1, 3
    • Yao Y 1
    • Zhang Y 1
    • Wang Y 1
    • Xu C 1
    • Liu X 1
    • Zhang C 1
    • Ding G 1
    • Guo B 1
    (10 authors)
  • 2. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
      Authors
    • Meng G 2
    • Zhang H 2
    (2 authors)
  • 3. Institute of Animal Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China.
      Authors
    • Luo Y 1, 3
    • Pang Y 3
    (2 authors)

ORCIDs linked to this article

Frontiers in Plant Science, 14 Nov 2022, 13:1034943
https://doi.org/10.3389/fpls.2022.1034943 PMID: 36452098 PMCID: PMC9702526

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Abstract 

Epimedium pubescens is a species of the family Berberidaceae in the basal eudicot lineage, and a main plant source for the traditional Chinese medicine "Herba Epimedii". The current study achieved a chromosome-level genome assembly of E. pubescens with the genome size of 3.34 Gb, and the genome guided discovery of a key prenyltransferase (PT) in E. pubescens. Our comparative genomic analyses confirmed the absence of Whole Genome Triplication (WGT-γ) event shared in core eudicots and further revealed the occurrence of an ancient Whole Genome Duplication (WGD) event approximately between 66 and 81 Million Years Ago (MYA). In addition, whole genome search approach was successfully applied to identify 19 potential flavonoid PT genes and an important flavonoid PT (EpPT8) was proven to be an enzyme for the biosynthesis of medicinal compounds, icaritin and its derivatives in E. pubescens. Therefore, our results not only provide a good reference genome to conduct further molecular biological studies in Epimedium genus, but also give important clues for synthetic biology and industrial production of related prenylated flavonoids in future.

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    Funding 

    Funders who supported this work.

    Chinese Academy of Medical Sciences (1)

    • Grant ID: 2021-I2M-1-031 , 2017-I2M-3-013

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    Chongqing Science and Technology Commission (1)

    • Grant ID: cstc2018jcyjAX0316, cc-cstc-CA-19-2, cstc2019jcyj-msxmx0464, cstc2019jxjl-jbky10007, cstc2020jxjl10004

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    National Natural Science Foundation of China (1)

    • Grant ID: 31570306, U20A2004, 81473302

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