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Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles.

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Affiliations

  • 1. Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Shanghai Cooperative Innovation Center for Modern Seed Industry, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
      Authors
    • Wu Y 1
    • Wang S 1
    • Wang P 1
    • Nie W 1
    • Chen G 1
    (5 authors)
  • 2. Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Shanghai Cooperative Innovation Center for Modern Seed Industry, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; Department of Environmental Sciences, COMSATS University Islamabad, Vehari-Campus, Vehari 61100, Pakistan.
      Authors
    • Ahmad I 2
    (1 author)
  • 3. Department of Biology, University of Crete, 714 09 Heraklion, Crete, Greece.
      Authors
    • Sarris PF 3
    (1 author)
  • 4. Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Shanghai Cooperative Innovation Center for Modern Seed Industry, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; Shanghai Jiao Tong University Chongqing Research Institute, Shanghai, China.
      Authors
    • Zhu B 4
    (1 author)

ORCIDs linked to this article

Plant Communications, 12 Jan 2024, 5(4):100817
https://doi.org/10.1016/j.xplc.2024.100817 PMID: 38217288 PMCID: PMC11009154

This article is in the Europe PMC Open access subset. Refer to the copyright information in the article for licensing details.
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This article has been corrected. See Plant Commun. 2024 Jul 8;5(7):101004. doi: 10.1016/j.xplc.2024.101004.

Abstract 

Noncoding small RNAs (sRNAs) packaged in bacterial outer membrane vesicles (OMVs) function as novel mediators of interspecies communication. While the role of bacterial sRNAs in enhancing virulence is well established, the role of sRNAs in the interaction between OMVs from phytopathogenic bacteria and their host plants remains unclear. In this study, we employ RNA sequencing to characterize differentially packaged sRNAs in OMVs of the phytopathogen Xanthomonas oryzae pv. oryzicola (Xoc). Our candidate sRNA (Xosr001) was abundant in OMVs and involved in the regulation of OsJMT1 to impair host stomatal immunity. Xoc loads Xosr001 into OMVs, which are specifically ttransferred into the mechanical tissues of rice leaves. Xosr001 suppresses OsJMT1 transcript accumulation in vivo, leading to a reduction in MeJA accumulation in rice leaves. Furthermore, the application of synthesized Xosr001 sRNA to the leaves of OsJMT1-HA-OE transgenic line results in the suppression of OsJMT1 expression by Xosr001. Notably, the OsJMT1-HA-OE transgenic line exhibited attenuated stomatal immunity and disease susceptibility upon infection with ΔXosr001 compared to Xoc. These results suggest that Xosr001 packaged in Xoc OMVs functions to suppress stomatal immunity in rice.

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    National Natural Science Foundation of China

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