Furthermore, molecular docking suggested favorable binding affinities between the key components/metabolites (e.g., Quercetin, 12,13-DHOME, PGE1) and core targets within these pathways (including PIK3CA, NOS3, PPARG, TNF, and RELA).<h4>Conclusion</h4>DSG demonstrated significant cardioprotective effects in vasospastic CHD rats, which were associated with coordinated gut microbiota remodeling, lipid-metabolic reprogramming, and modulation of inflammation-related pathways. The gene discussed is PIK3CA; the disease is coronary artery disorder.