The resulting iPSC lines underwent comprehensive characterization, including alkaline phosphatase (AP) staining, immunofluorescence (IF) for pluripotency markers (e.g., OCT-4, SOX-2, and TRA-1-60), quantitative reverse transcription real-time PCR (RT-qPCR) for the expression of pluripotency genes, G-band karyotyping, and genetic analysis for thalassemia. This evidence concerns the gene POU5F1 and thalassemia.