In HEK293T cells, Rikkunshito inhibited TRPV1, slightly enhanced TRPV4, and robustly activated TRPA1 currents.<h4>Conclusion</h4>Rikkunshito exerts protective effects in IBS by coordinately regulating colonic pacemaker activity via ghrelin receptor-dependent Ca<sup>2+</sup> signaling, modulating neuroendocrine balance, improving epithelial barrier function, and differentially regulating TRP channel activity. The gene discussed is GHSR; the disease is irritable bowel syndrome.