THBS2 and endometriosis: qPCR and Western blot analyses confirmed significant upregulation of COL6A3, BGN, LAMA4, and THBS2 at both transcriptional and protein levels in ectopic endometrium, further supporting their role in disease pathogenesis.<h4>Conclusion</h4>COL6A3, BGN, LAMA4, and THBS2 represent promising candidate tissue diagnostic markers for endometriosis, linked to extracellular matrix and immune microenvironment alterations, providing novel insights for future research and clinical translation.