BIRC5 function was evaluated through overexpression and siRNA-mediated knockdown in retinoblastoma cell lines (Y79, WERI-Rb1), with assessment of proliferation, migration, invasion, and cell cycle-related proteins.<h4>Results</h4>Clustering identified 10 retinal cell types, including cone precursor-like, cone-like, and two MKI67<sup>+</sup> proliferative RB subpopulations enrichment of G2/M-phase genes and chromosomal instability pathways. The gene discussed is MKI67; the disease is retinoblastoma.