Synaptosomes from neuropathologically confirmed MND motor cortex and hSOD1<sup>G93A</sup> mouse cortex showed significantly greater depolarisation-evoked Ca<sup>2+</sup> entry than their respective controls, suggesting that increased presynaptic Ca<sup>2+</sup> influx is shared across our human MND cohort and the hSOD1<sup>G93A</sup> mouse model. Here, SOD1 is linked to mild neurocognitive disorder.