Our objective was to elucidate the correlation between computational predictions and functional outcomes.<h4>Methods</h4>A CRISPR-Cas9 knock-in approach was used to introduce and generate specific point mutations in Exons 21, 22, and 23 of <i>MSH3</i> in the wild-type (WT) <i>MSH3</i> CRC cell line SW620, which was confirmed with Sanger sequencing. Here, MSH3 is linked to colorectal carcinoma.