The study is aimed at determining the molecular spectrum of hemoglobinopathies and contributing to the development of a comprehensive Hb mutation map in Tunisia.<h4>Results</h4>Twenty-one <i>β</i>-thalassemia mutations and 16 rare Hb variants were reported, affecting the HBA, HBD, and HBB genes. Here, GSTM1 is linked to hemoglobinopathy.