Additionally, spatial trajectory analysis indicated shared and distinct gene expression patterns during differentiation toward peritumoral and intratumoral immune regions, revealing a complex landscape of NPC invasion.<h4>Conclusion</h4>Our findings suggest that glycerophospholipid metabolism may play an important role in NPC progression and highlight the potential of AGPAT3, DGAT2, SLC44A1, AGPAT5, and LPGAT1 as diagnostic and prognostic markers. Here, DGAT2 is linked to nasopharyngeal carcinoma.