<h4>Background</h4>This study investigated whether peripheral blood expression of linear ubiquitin chain assembly complex (LUBAC) and OTU deubiquitinase with linear linkage specificity (OTULIN) is associated with stroke severity and functional outcome in acute ischemic stroke (AIS) patients.<h4>Methods</h4>Immunofluorescence for LUBAC and OTULIN was performed in cortical autopsy specimens from two AIS cases. The gene discussed is OTULIN; the disease is stroke disorder.