This study aims to explore the dynamic changes in serum and follicular fluid (FF) Kisspeptin levels during COH in PCOS patients and analyze their correlation with COH outcomes and pregnancy outcomes, in order to evaluate the potential value of Kisspeptin as a predictive biomarker.<h4>Methods</h4>This prospective cohort study included 100 patients undergoing IVF-ET treatment (50 in the PCOS group and 50 in the control group). This evidence concerns the gene KISS1 and polycystic ovary syndrome.