<h4>Background</h4>This study aimed to investigate the effects of γδ T cell inhibition under a high-fat diet (HFD) on metabolic function, immune inflammation, gut microbiota, and atherosclerosis (AS) progression in <i>ApoE</i> <sup>-/-</sup> mice.<h4>Methods</h4><i>ApoE</i> <sup>-/-</sup> mice were assigned to three groups: a control group (normal diet), a model group (HFD), and an intervention group (HFD + γδ T cell receptor (TCR) monoclonal antibody). This evidence concerns the gene APOE and atherosclerosis.