Inflammatory markers in blood and adipose tissue were quantified, gut microbiota composition was analyzed via fecal metagenomics, and atherosclerosis was evaluated using Oil Red O, Masson's trichrome, and hematoxylin and eosin (HE) staining methods.<h4>Results</h4>The HFD activated γδ T cells and increased pro-inflammatory cytokines in <i>ApoE</i> <sup>-/-</sup> mice. This evidence concerns the gene APOE and atherosclerosis.