In vivo experiments confirmed differential protein abundance of PADI4, POLB, and IFI27 in CLP-induced ALI lungs, supporting their potential as biomarkers.<h4>Conclusion</h4>Integrating bulk and single-cell transcriptomes delineated PCD-associated molecular features in sepsis-induced ALI and identified an externally validated 8-gene classifier signature. Here, POLB is linked to Sepsis.