<h4>Introduction</h4>Whether cholinergic activity in the septohippocampal network affects cognitive dysfunction via hippocampal cholinergic neurostimulating peptide (HCNP) in Alzheimer's pathogenesis remains unclear.<h4>Methods</h4>An Alzheimer's pathogenesis by mutation in amyloid-beta precursor protein gene (App<sup>NL-G-F</sup>) knock-in (KI) and HCNP precursor protein conditional knockout (HCNP-pp cKO) mouse model was generated, exhibiting both cholinergic dysfunction and amyloid pathogenesis. The gene discussed is APP; the disease is amyloidosis.