YBX1 and IGF2BP1 also physically interacted, co-occupied EPHB4 transcripts, and reciprocally enhanced m5C- and m6A-associated EPHB4 RNA enrichment.<h4>Conclusion</h4>These findings reveal a cooperative epitranscriptomic mechanism in which NSUN2/YBX1-mediated m<sup>5</sup>C signaling and IGF2BP1-associated m<sup>6</sup>A recognition converge on EPHB4 mRNA to promote gastric cancer lymphatic metastasis, highlighting the NSUN2-YBX1-IGF2BP1-EPHB4 axis as a potential therapeutic target. This evidence concerns the gene IGF2BP1 and gastric cancer.