Bootstrap internal validation yielded a mean optimism-corrected AUC of 0.861 (95% CI: 0.812-0.903), indicating robust model performance with minimal overfitting.<h4>Conclusion</h4>Clinical variables alongside altered levels of Hcrt, NfH, and IL-1β serve as independent predictors of post-stroke sleep disorders. The gene discussed is HCRT; the disease is Stroke.