This study aimed to explore the characteristics of gut microbiota alterations during DKD progression and evaluate whether CD4<sup>+</sup> T cell count showed a statistically significant mediating association in this process.<h4>Methods</h4>A total of 157 patients with DKD were classified into early (n=34), middle (n=91) and late (n=32) stage groups according to clinical indicators. The gene discussed is CD4; the disease is diabetic kidney disease.