Key findings were validated through <i>in vitro</i> experiments using cervical cancer cell lines, macrophage polarization assays, metabolic measurements, ELISA, immunofluorescence, and CD8<sup>+</sup> T cell functional analyses.<h4>Results</h4>Single-cell analysis revealed profound differences in cellular composition and immune states between HPV-negative and HPV-positive adenocarcinomas. This evidence concerns the gene CD8A and adenocarcinoma.