Key findings were validated through <i>in vitro</i> experiments using cervical cancer cell lines, macrophage polarization assays, metabolic measurements, ELISA, immunofluorescence, and CD8<sup>+</sup> T cell functional analyses.<h4>Results</h4>Single-cell analysis revealed profound differences in cellular composition and immune states between HPV-negative and HPV-positive adenocarcinomas. The gene discussed is CD8A; the disease is cervical cancer.