This study investigated whether Gardenoside preserves BBB integrity after ischemic stroke via AMPK-dependent regulation of the tight junction protein ZO-1.<h4>Methods</h4>Male and female C57BL/6 mice (8-10 weeks) underwent transient middle cerebral artery occlusion (MCAO; 60 min ischemia, 24 h reperfusion). The gene discussed is TJP1; the disease is ischemic stroke.