An osteosarcoma xenograft model was established; tumor expression of ACSL4, HO‐1, and Ki67 was examined by IHC, and serum ferritin levels were measured by ELISA.<h4>Results</h4>Bioinformatics analysis of the GEO dataset (GSE42352) revealed a negative correlation between Wnt target gene expression and ferroptosis‐related gene signatures, suggesting that ferroptosis may be involved. The gene discussed is HMOX1; the disease is osteosarcoma.