PLK1 and neoplasm: While nitidine chloride (NC) exhibits anti-tumor effects, its molecular targets in CRC are still largely unknown.<h4>Methods</h4>To delineate the role of Polo-like kinase 1 (PLK1) in CRC, we combined RNA sequencing with comprehensive multi-omics integration across 3,513 specimens (2,256 tumor and 1,257 non-tumor).