In vivo validation employed a ligature-induced periodontitis rat model with TRP irrigation treatment, evaluated through micro-CT, histological staining (H&E and TRAP), and molecular analyses.<h4>Results</h4>Network pharmacology identified 129 potential targets of TRP for treating periodontitis, with PI3K/AKT emerging as a key signalling pathway. This evidence concerns the gene AKT1 and periodontitis.