Three-dimensional spatial colocalization of α5-GABAAR and gephyrin was reconstructed in IMARIS software.<h4>Results</h4>By integrating trace fear conditioning with molecular profiling, we identified 2 days postexposure (Sev2d) as the critical phase for sevoflurane-induced memory impairment and 6 days postexposure (Sev6d) as the recovery phase. This evidence concerns the gene GPHN and memory impairment.