<b>Conclusions</b>: This study expands Pakistan's non-syndromic RP mutational spectrum by identifying novel variants in <i>RP1, PDE6B</i>, and <i>PRCD,</i> alongside recurrent <i>CERKL</i> and <i>RHO</i> mutations of the local population. This evidence concerns the gene PDE6B and retinitis pigmentosa 1.