CAFs have been shown to promote protumoral effects in CC cell lines; however, the effects of E2-stimulated CAFs remain to be explored.<h4>Objectives</h4>This study aimed to evaluate whether E2-stimulated CAFs modify the behavior of SiHa and HeLa tumor cells, including their metabolism, ROS production, migration, apoptosis, and gene expression.<h4>Methods</h4>Characterization and estrogen receptor expression in primary CAF cultures were evaluated by immunofluorescence. Here, ESR1 is linked to neoplasm.