ATP-binding cassette superfamily B1 (<i>ABCB1</i>) not only has a potential influence on the resistance to antiepileptic drugs but also plays a possible role in the occurrence of epilepsy.<h4>Purpose</h4>To evaluate the association of <i>ABCB1</i> polymorphisms, c.1236C>T (rs1128503), c.2677G>T (rs2032582), and c.3435C>T (rs1045642), with epilepsy susceptibility in a Jordanian cohort.<h4>Subjects and methods</h4>Eighty-six cases of patients with epilepsy were analyzed using polymerase chain reaction (PCR) for <i>ABCB1</i> c.1236C>T, c.2677G>T, and c.3435C>T gene variants. The gene discussed is ABCB1; the disease is epilepsy.