On day 30, no significant intergroup differences were found for PerioW or immunohistochemical parameters; however, PtRt continued to show the highest PerioW and OPG values and the lowest RANKL/OPG ratio.<h4>Conclusions</h4>Radiotherapy superimposed on periodontitis enhanced early pro-resorptive signaling and delayed structural normalization of periodontal tissues. The gene discussed is TNFSF11; the disease is periodontitis.