The mRNA levels of prognostic genes were examined in MCF-10A mammary epithelial cells and breast cancer cell lines.<h4>Results</h4>We developed and validated a novel seven-gene, metastasis-associated prognostic signature (<i>IGJ</i>, <i>CXCL14</i>, <i>PTGER3</i>, <i>RTN1</i>, <i>EGOT</i>, <i>TLR10</i>, <i>PANX2</i>) for breast cancer. The gene discussed is PTGER3; the disease is breast cancer.