We screened for established pathogenic variants with variant allele fraction (VAF) < 8% in dominant CHI (ABCC8, GCK, GLUD1, HK1) or dominant NDM (ABCC8, KCNJ11, INS) genes in targeted next-generation sequencing (tNGS) data using Mutect2.<h4>Findings</h4>This called 40 variants across the four genes in 39 individuals with CHI. Here, GCK is linked to congenital isolated hyperinsulinism.