Using <sup>NF-κB</sup>Luc-reporter mice, we longitudinally monitored NF-κB activation by using in vivo bioluminescence imaging in the tumor microenvironment (TME) and bone marrow (BM) of mice bearing OVA-expressing MC38 adenocarcinoma (responder) or B16 melanoma (non-responder). Here, NFKB1 is linked to melanoma.