Importantly, constitutive activation of Akt partially rescued ATN-induced cytotoxicity (~78% recovery), confirming the involvement of YAP-associated EGFR/Akt signaling.<h4>Conclusion</h4>ATN exerts potent anti-tumor effects against CRPC cells by activating the Hippo pathway, promoting YAP degradation, and disrupting the YAP-associated EGFR signaling network. The gene discussed is EGFR; the disease is neoplasm.