This study thus investigated how NTN1 regulates S-AKI.<h4>Methods</h4>HK-2 cells were exposed to lipopolysaccharides (LPS), where NTN1 expression was manipulated to study the effects of NTN1 on apoptosis, inflammatory factors (tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6), and reactive oxygen species (ROS) levels. The gene discussed is TNF; the disease is acute kidney injury.