CHRNA3 and colitis: Pharmacological inhibition of CHRNA3+ MINs via pERK blockade or chemogenetic silencing alleviated mechanical hypersensitivity of BL25 and attenuated the analgesic effect of EA on both visceral pain and spinal sensitization.<h4>Conclusion</h4>Our findings reveal that CHRNA3+ silent nociceptors dynamically prime and reshape the sensory interface of the colitis-induced sensitized acupoint BL25, subsequently facilitating both pathological hypersensitivity and therapeutic responsiveness by heightening mechano-responsiveness of the acupoint.