Ultimately, qRT-PCR results revealed that NCAPH was in line with the bioinformatics results.<h4>Conclusion</h4>This study discovered causal effect of obesity on NAFLD and identified two shared biomarkers (NCAPH and IRS2) in two diseases, providing new insights for further explore the pathogenesis of both diseases. Here, IRS2 is linked to obesity due to melanocortin 4 receptor deficiency.