Functional assays, including wound healing, transwell migration, and invasion assays, were conducted following siRNA-mediated knockdown of selected hub genes in Huh7 hepatoma cells.<h4>Results</h4>We identified five key xenobiotic metabolism-related hub genes: AKR1C3, CYB5A, ADH1C, MAOA, and ALDH2. Here, AKR1C3 is linked to hepatocellular carcinoma.