For stratifying low-positive from high-positive anti-dsDNA titers, the combined assessment showed better discriminatory performance than PNI and C3.<h4>Conclusions</h4>Combined assessment of 25(OH)D, the CD4<sup>+</sup>/CD8<sup>+</sup> ratio, and PNI may provide complementary value for anti-dsDNA-based stratification in SLE. This evidence concerns the gene CD4 and systemic lupus erythematosus.