However, the relationships of 25-hydroxyvitamin D [25(OH)D], CD4<sup>+</sup>/CD8<sup>+</sup> ratio, and prognostic nutritional index (PNI) with anti-dsDNA antibody status and titer stratification remain incompletely understood.<h4>Methods</h4>A total of 126 patients with SLE were enrolled and classified into an anti-dsDNA-negative group and an anti-dsDNA-positive group. The gene discussed is CD4; the disease is systemic lupus erythematosus.