CD34 and essential thrombocythemia: Functional assays including shRNA knockdown, CCK-8, apoptosis detection, and Western blot were conducted in primary CD34+ cell-derived megakaryocytes from ET patients.<h4>Results</h4>Eleven key DEGs were identified, and the logistic regression model achieved area under the curve of 0.846 and 0.863 for the training and test sets, respectively.